Evolutionary engineering of Saccharomyces cerevisiae for efficient conversion of red algal biosugars to bioethanol

Hye Jin Lee; Soo Jung Kim; Jeong Jun Yoon; Kyoung Heon Kim; Jin Ho Seo; Yong Cheol Park

doi:10.1016/j.biortech.2015.03.057

Evolutionary engineering of Saccharomyces cerevisiae for efficient conversion of red algal biosugars to bioethanol

Hye Jin Lee, Soo Jung Kim, Jeong Jun Yoon, Kyoung Heon Kim, Jin Ho Seo, Yong Cheol Park

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The aim of this work was to apply the evolutionary engineering to construct a mutant Saccharomyces cerevisiae HJ7-14 resistant on 2-deoxy-d-glucose and with an enhanced ability of bioethanol production from galactose, a mono-sugar in red algae. In batch and repeated-batch fermentations, HJ7-14 metabolized 5-fold more galactose and produced ethanol 2.1-fold faster than the parental D452-2 strain. Transcriptional analysis of genes involved in the galactose metabolism revealed that moderate relief from the glucose-mediated repression of the transcription of the GAL genes might enable HJ7-14 to metabolize galactose rapidly. HJ7-14 produced 7.4 g/L ethanol from hydrolysates of the red alga Gelidium amansii within 12 h, which was 1.5-times faster than that observed with D452-2. We demonstrate conclusively that evolutionary engineering is a promising tool to manipulate the complex galactose metabolism in S. cerevisiae to produce bioethanol from red alga.

Original language	English
Pages (from-to)	445-451
Number of pages	7
Journal	Bioresource technology
Volume	191
DOIs	https://doi.org/10.1016/j.biortech.2015.03.057
Publication status	Published - 2015 Jun 8

Keywords

Bioethanol
Evolutionary engineering
Galactose
Red algae
Saccharomyces cerevisiae

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2015.03.057

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title = "Evolutionary engineering of Saccharomyces cerevisiae for efficient conversion of red algal biosugars to bioethanol",

abstract = "The aim of this work was to apply the evolutionary engineering to construct a mutant Saccharomyces cerevisiae HJ7-14 resistant on 2-deoxy-d-glucose and with an enhanced ability of bioethanol production from galactose, a mono-sugar in red algae. In batch and repeated-batch fermentations, HJ7-14 metabolized 5-fold more galactose and produced ethanol 2.1-fold faster than the parental D452-2 strain. Transcriptional analysis of genes involved in the galactose metabolism revealed that moderate relief from the glucose-mediated repression of the transcription of the GAL genes might enable HJ7-14 to metabolize galactose rapidly. HJ7-14 produced 7.4 g/L ethanol from hydrolysates of the red alga Gelidium amansii within 12 h, which was 1.5-times faster than that observed with D452-2. We demonstrate conclusively that evolutionary engineering is a promising tool to manipulate the complex galactose metabolism in S. cerevisiae to produce bioethanol from red alga.",

keywords = "Bioethanol, Evolutionary engineering, Galactose, Red algae, Saccharomyces cerevisiae",

author = "Lee, {Hye Jin} and Kim, {Soo Jung} and Yoon, {Jeong Jun} and Kim, {Kyoung Heon} and Seo, {Jin Ho} and Park, {Yong Cheol}",

note = "Funding Information: This work was financially supported by The Advanced Biomass R&D Center (ABC) of Korea Grant ( 2011-0031359 ) and also by the National Research Foundation of Korea ( NRF ) Grant ( 2013R1A2A2A01004806 and 2013M1A2A2072603 ) funded by the Korean Ministry of Science, ICT and Future Planning . Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd All rights reserved.",

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doi = "10.1016/j.biortech.2015.03.057",

language = "English",

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AU - Lee, Hye Jin

AU - Kim, Soo Jung

AU - Yoon, Jeong Jun

AU - Kim, Kyoung Heon

AU - Seo, Jin Ho

AU - Park, Yong Cheol

N1 - Funding Information: This work was financially supported by The Advanced Biomass R&D Center (ABC) of Korea Grant ( 2011-0031359 ) and also by the National Research Foundation of Korea ( NRF ) Grant ( 2013R1A2A2A01004806 and 2013M1A2A2072603 ) funded by the Korean Ministry of Science, ICT and Future Planning . Publisher Copyright: © 2015 Elsevier Ltd All rights reserved.

PY - 2015/6/8

Y1 - 2015/6/8

N2 - The aim of this work was to apply the evolutionary engineering to construct a mutant Saccharomyces cerevisiae HJ7-14 resistant on 2-deoxy-d-glucose and with an enhanced ability of bioethanol production from galactose, a mono-sugar in red algae. In batch and repeated-batch fermentations, HJ7-14 metabolized 5-fold more galactose and produced ethanol 2.1-fold faster than the parental D452-2 strain. Transcriptional analysis of genes involved in the galactose metabolism revealed that moderate relief from the glucose-mediated repression of the transcription of the GAL genes might enable HJ7-14 to metabolize galactose rapidly. HJ7-14 produced 7.4 g/L ethanol from hydrolysates of the red alga Gelidium amansii within 12 h, which was 1.5-times faster than that observed with D452-2. We demonstrate conclusively that evolutionary engineering is a promising tool to manipulate the complex galactose metabolism in S. cerevisiae to produce bioethanol from red alga.

AB - The aim of this work was to apply the evolutionary engineering to construct a mutant Saccharomyces cerevisiae HJ7-14 resistant on 2-deoxy-d-glucose and with an enhanced ability of bioethanol production from galactose, a mono-sugar in red algae. In batch and repeated-batch fermentations, HJ7-14 metabolized 5-fold more galactose and produced ethanol 2.1-fold faster than the parental D452-2 strain. Transcriptional analysis of genes involved in the galactose metabolism revealed that moderate relief from the glucose-mediated repression of the transcription of the GAL genes might enable HJ7-14 to metabolize galactose rapidly. HJ7-14 produced 7.4 g/L ethanol from hydrolysates of the red alga Gelidium amansii within 12 h, which was 1.5-times faster than that observed with D452-2. We demonstrate conclusively that evolutionary engineering is a promising tool to manipulate the complex galactose metabolism in S. cerevisiae to produce bioethanol from red alga.

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KW - Evolutionary engineering

KW - Galactose

KW - Red algae

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Evolutionary engineering of Saccharomyces cerevisiae for efficient conversion of red algal biosugars to bioethanol

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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