Ex situ product recovery and strain engineering of Clostridium acetobutylicum for enhanced production of butanol

Sang Hyun Lee, Moon Ho Eom, Sooah Kim, Min A. Kwon, Jin Dal Rae Choi, Jungyeon Kim, Yong An Shin, Kyoung Heon Kim

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


To reduce butanol toxicity, adsorptive fermentation has been extensively studied. In this study, SP850 was systematically selected as an optimal adsorbent showing high butanol selectivity. In the flask culture involving in situ butanol recovery fermentation using Clostridium acetobutylicum ATCC 824 and the adsorbent, production of acetone, butanol, and ethanol (ABE) increased by 46.8%. An acid crash, however, occurred in the ex situ recovery fermentation using SP850. This problem was solved by homologous expression of the adhE1 gene in the ATCC 824 strain. During the ex situ recovery fermentation by the adhE-overexpressing strain, 34.2 g/L of ABE was produced from 132.8 g/L of glucose. In the 146-h continuous mode of ex situ fermentation, the strain produced 205.6 g/L of ABE (146.9 g/L of butanol) from 605.3 g/L of glucose. Our results indicate that proper selection of an adsorbent and strain engineering for the ex situ recovery fermentation is effective at enhancing butanol fermentation.

Original languageEnglish
Pages (from-to)1683-1691
Number of pages9
JournalProcess Biochemistry
Issue number11
Publication statusPublished - 2015 Nov

Bibliographical note

Funding Information:
This work was supported by the Center for Organic Waste-to-Energy Business under the Environmental Technology Development Program funded by the Ministry of Environment (2013001580001). KHK acknowledges the facility support at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety.

Publisher Copyright:
© 2015 Elsevier Ltd.

Copyright 2015 Elsevier B.V., All rights reserved.


  • Adsorbent
  • Butanol
  • Clostridium acetobutylicum
  • Ex situ butanol recovery

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology


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