Ex situ product recovery and strain engineering of Clostridium acetobutylicum for enhanced production of butanol

Sang Hyun Lee; Moon Ho Eom; Sooah Kim; Min A. Kwon; Jin Dal Rae Choi; Jungyeon Kim; Yong An Shin; Kyoung Heon Kim

doi:10.1016/j.procbio.2015.08.010

Ex situ product recovery and strain engineering of Clostridium acetobutylicum for enhanced production of butanol

Sang Hyun Lee, Moon Ho Eom, Sooah Kim, Min A. Kwon, Jin Dal Rae Choi, Jungyeon Kim, Yong An Shin, Kyoung Heon Kim

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

To reduce butanol toxicity, adsorptive fermentation has been extensively studied. In this study, SP850 was systematically selected as an optimal adsorbent showing high butanol selectivity. In the flask culture involving in situ butanol recovery fermentation using Clostridium acetobutylicum ATCC 824 and the adsorbent, production of acetone, butanol, and ethanol (ABE) increased by 46.8%. An acid crash, however, occurred in the ex situ recovery fermentation using SP850. This problem was solved by homologous expression of the adhE1 gene in the ATCC 824 strain. During the ex situ recovery fermentation by the adhE-overexpressing strain, 34.2 g/L of ABE was produced from 132.8 g/L of glucose. In the 146-h continuous mode of ex situ fermentation, the strain produced 205.6 g/L of ABE (146.9 g/L of butanol) from 605.3 g/L of glucose. Our results indicate that proper selection of an adsorbent and strain engineering for the ex situ recovery fermentation is effective at enhancing butanol fermentation.

Original language	English
Pages (from-to)	1683-1691
Number of pages	9
Journal	Process Biochemistry
Volume	50
Issue number	11
DOIs	https://doi.org/10.1016/j.procbio.2015.08.010
Publication status	Published - 2015 Nov

Bibliographical note

Funding Information:
This work was supported by the Center for Organic Waste-to-Energy Business under the Environmental Technology Development Program funded by the Ministry of Environment (2013001580001). KHK acknowledges the facility support at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety.

Publisher Copyright:
© 2015 Elsevier Ltd.

Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.

Keywords

Adsorbent
Butanol
Clostridium acetobutylicum
Ex situ butanol recovery

ASJC Scopus subject areas

Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.procbio.2015.08.010

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title = "Ex situ product recovery and strain engineering of Clostridium acetobutylicum for enhanced production of butanol",

abstract = "To reduce butanol toxicity, adsorptive fermentation has been extensively studied. In this study, SP850 was systematically selected as an optimal adsorbent showing high butanol selectivity. In the flask culture involving in situ butanol recovery fermentation using Clostridium acetobutylicum ATCC 824 and the adsorbent, production of acetone, butanol, and ethanol (ABE) increased by 46.8%. An acid crash, however, occurred in the ex situ recovery fermentation using SP850. This problem was solved by homologous expression of the adhE1 gene in the ATCC 824 strain. During the ex situ recovery fermentation by the adhE-overexpressing strain, 34.2 g/L of ABE was produced from 132.8 g/L of glucose. In the 146-h continuous mode of ex situ fermentation, the strain produced 205.6 g/L of ABE (146.9 g/L of butanol) from 605.3 g/L of glucose. Our results indicate that proper selection of an adsorbent and strain engineering for the ex situ recovery fermentation is effective at enhancing butanol fermentation.",

keywords = "Adsorbent, Butanol, Clostridium acetobutylicum, Ex situ butanol recovery",

author = "Lee, {Sang Hyun} and Eom, {Moon Ho} and Sooah Kim and Kwon, {Min A.} and Choi, {Jin Dal Rae} and Jungyeon Kim and Shin, {Yong An} and Kim, {Kyoung Heon}",

note = "Funding Information: This work was supported by the Center for Organic Waste-to-Energy Business under the Environmental Technology Development Program funded by the Ministry of Environment (2013001580001). KHK acknowledges the facility support at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety. Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.",

year = "2015",

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doi = "10.1016/j.procbio.2015.08.010",

language = "English",

volume = "50",

pages = "1683--1691",

journal = "Process Biochemistry",

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AU - Lee, Sang Hyun

AU - Eom, Moon Ho

AU - Kim, Sooah

AU - Kwon, Min A.

AU - Choi, Jin Dal Rae

AU - Kim, Jungyeon

AU - Shin, Yong An

AU - Kim, Kyoung Heon

N1 - Funding Information: This work was supported by the Center for Organic Waste-to-Energy Business under the Environmental Technology Development Program funded by the Ministry of Environment (2013001580001). KHK acknowledges the facility support at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety. Publisher Copyright: © 2015 Elsevier Ltd. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.

PY - 2015/11

Y1 - 2015/11

N2 - To reduce butanol toxicity, adsorptive fermentation has been extensively studied. In this study, SP850 was systematically selected as an optimal adsorbent showing high butanol selectivity. In the flask culture involving in situ butanol recovery fermentation using Clostridium acetobutylicum ATCC 824 and the adsorbent, production of acetone, butanol, and ethanol (ABE) increased by 46.8%. An acid crash, however, occurred in the ex situ recovery fermentation using SP850. This problem was solved by homologous expression of the adhE1 gene in the ATCC 824 strain. During the ex situ recovery fermentation by the adhE-overexpressing strain, 34.2 g/L of ABE was produced from 132.8 g/L of glucose. In the 146-h continuous mode of ex situ fermentation, the strain produced 205.6 g/L of ABE (146.9 g/L of butanol) from 605.3 g/L of glucose. Our results indicate that proper selection of an adsorbent and strain engineering for the ex situ recovery fermentation is effective at enhancing butanol fermentation.

AB - To reduce butanol toxicity, adsorptive fermentation has been extensively studied. In this study, SP850 was systematically selected as an optimal adsorbent showing high butanol selectivity. In the flask culture involving in situ butanol recovery fermentation using Clostridium acetobutylicum ATCC 824 and the adsorbent, production of acetone, butanol, and ethanol (ABE) increased by 46.8%. An acid crash, however, occurred in the ex situ recovery fermentation using SP850. This problem was solved by homologous expression of the adhE1 gene in the ATCC 824 strain. During the ex situ recovery fermentation by the adhE-overexpressing strain, 34.2 g/L of ABE was produced from 132.8 g/L of glucose. In the 146-h continuous mode of ex situ fermentation, the strain produced 205.6 g/L of ABE (146.9 g/L of butanol) from 605.3 g/L of glucose. Our results indicate that proper selection of an adsorbent and strain engineering for the ex situ recovery fermentation is effective at enhancing butanol fermentation.

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KW - Ex situ butanol recovery

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Ex situ product recovery and strain engineering of Clostridium acetobutylicum for enhanced production of butanol

Abstract

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Keywords

ASJC Scopus subject areas

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