Exon junction complex enhances translation of spliced mRNAs at multiple steps

Hyung Chul Lee; Junho Choe; Sung Gil Chi; Yoon Ki Kim

doi:10.1016/j.bbrc.2009.04.123

Exon junction complex enhances translation of spliced mRNAs at multiple steps

Hyung Chul Lee, Junho Choe, Sung Gil Chi, Yoon Ki Kim

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Translation of spliced mRNAs is enhanced by exon junction complex (EJC), which is deposited on mRNAs as a result of splicing. Although this phenomenon itself is well known, the underlying molecular mechanism remains poorly understood. Here we show, using siRNAs against Y14 and eIF4AIII and spliced or intronless constructs that contain different types of internal ribosome entry sites (IRESes), that Y14 and eIF4AIII increase translation of spliced mRNAs before and after formation of the 80S ribosome complex, respectively. These results suggest that EJC modulates translation of spliced mRNA at multiple steps.

Original language	English
Pages (from-to)	334-340
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	384
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2009.04.123
Publication status	Published - 2009 Jul 3

Bibliographical note

Funding Information:
We thank L.E. Maquat, N. Sonenberg, R. Reed, G. Dreyfuss, and P. Sarnow for antibodies and plasmids. This work was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A062356), the Korea Science and Engineering Foundation (KOSEF) Grant funded by the Korea government (MEST) (No. 2009-0078061), and the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2008-314-C00247).

Keywords

Exon junction complex
Internal ribosome entry site
Nonsense-mediated mRNA decay
Splicing
Y14
eIF4AIII

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2009.04.123

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abstract = "Translation of spliced mRNAs is enhanced by exon junction complex (EJC), which is deposited on mRNAs as a result of splicing. Although this phenomenon itself is well known, the underlying molecular mechanism remains poorly understood. Here we show, using siRNAs against Y14 and eIF4AIII and spliced or intronless constructs that contain different types of internal ribosome entry sites (IRESes), that Y14 and eIF4AIII increase translation of spliced mRNAs before and after formation of the 80S ribosome complex, respectively. These results suggest that EJC modulates translation of spliced mRNA at multiple steps.",

keywords = "Exon junction complex, Internal ribosome entry site, Nonsense-mediated mRNA decay, Splicing, Y14, eIF4AIII",

author = "Lee, {Hyung Chul} and Junho Choe and Chi, {Sung Gil} and Kim, {Yoon Ki}",

note = "Funding Information: We thank L.E. Maquat, N. Sonenberg, R. Reed, G. Dreyfuss, and P. Sarnow for antibodies and plasmids. This work was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A062356), the Korea Science and Engineering Foundation (KOSEF) Grant funded by the Korea government (MEST) (No. 2009-0078061), and the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2008-314-C00247). ",

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AU - Lee, Hyung Chul

AU - Choe, Junho

AU - Chi, Sung Gil

AU - Kim, Yoon Ki

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AB - Translation of spliced mRNAs is enhanced by exon junction complex (EJC), which is deposited on mRNAs as a result of splicing. Although this phenomenon itself is well known, the underlying molecular mechanism remains poorly understood. Here we show, using siRNAs against Y14 and eIF4AIII and spliced or intronless constructs that contain different types of internal ribosome entry sites (IRESes), that Y14 and eIF4AIII increase translation of spliced mRNAs before and after formation of the 80S ribosome complex, respectively. These results suggest that EJC modulates translation of spliced mRNA at multiple steps.

KW - Exon junction complex

KW - Internal ribosome entry site

KW - Nonsense-mediated mRNA decay

KW - Splicing

KW - Y14

KW - eIF4AIII

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Exon junction complex enhances translation of spliced mRNAs at multiple steps

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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