Expansion of the prime editing modality with Cas9 from Francisella novicida

Yeounsun Oh; Wi jae Lee; Junho K. Hur; Woo Jeung Song; Youngjeon Lee; Hanseop Kim; Lee Wha Gwon; Young Hyun Kim; Young Ho Park; Chan Hyoung Kim; Kyung Seob Lim; Bong Seok Song; Jae Won Huh; Sun Uk Kim; Bong Hyun Jun; Cheulhee Jung; Seung Hwan Lee

doi:10.1186/s13059-022-02644-8

Expansion of the prime editing modality with Cas9 from Francisella novicida

Yeounsun Oh
, Wi jae Lee
, Junho K. Hur
, Woo Jeung Song
, Youngjeon Lee
, Hanseop Kim
, Lee Wha Gwon
, Young Hyun Kim
, Young Ho Park
, Chan Hyoung Kim
, Kyung Seob Lim
, Bong Seok Song
, Jae Won Huh
, Sun Uk Kim
, Bong Hyun Jun
, Cheulhee Jung^*
, Seung Hwan Lee^*

^*Corresponding author for this work

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase after nick formation by CRISPR nickase. In this study, we develop a technology that can be used to insert or replace external bases in the target DNA sequence by linking reverse transcriptase to the Francisella novicida Cas9, which is a CRISPR-Cas9 ortholog. Using FnCas9(H969A) nickase, the targeting limitation of existing Streptococcus pyogenes Cas9 nickase [SpCas9(H840A)]-based prime editing is dramatically extended, and accurate prime editing is induced specifically for the target genes in human cell lines.

Original language	English
Article number	92
Journal	Genome Biology
Volume	23
Issue number	1
DOIs	https://doi.org/10.1186/s13059-022-02644-8
Publication status	Published - 2022 Dec

Bibliographical note

Publisher Copyright:
© 2022, The Author(s).

Keywords

CRISPR-Cas9
Francisella novicida
Ortholog
Prime editing
Target expansion

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics
Cell Biology

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10.1186/s13059-022-02644-8

Cite this

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title = "Expansion of the prime editing modality with Cas9 from Francisella novicida",

abstract = "Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase after nick formation by CRISPR nickase. In this study, we develop a technology that can be used to insert or replace external bases in the target DNA sequence by linking reverse transcriptase to the Francisella novicida Cas9, which is a CRISPR-Cas9 ortholog. Using FnCas9(H969A) nickase, the targeting limitation of existing Streptococcus pyogenes Cas9 nickase [SpCas9(H840A)]-based prime editing is dramatically extended, and accurate prime editing is induced specifically for the target genes in human cell lines.",

keywords = "CRISPR-Cas9, Francisella novicida, Ortholog, Prime editing, Target expansion",

author = "Yeounsun Oh and Lee, \{Wi jae\} and Hur, \{Junho K.\} and Song, \{Woo Jeung\} and Youngjeon Lee and Hanseop Kim and Gwon, \{Lee Wha\} and Kim, \{Young Hyun\} and Park, \{Young Ho\} and Kim, \{Chan Hyoung\} and Lim, \{Kyung Seob\} and Song, \{Bong Seok\} and Huh, \{Jae Won\} and Kim, \{Sun Uk\} and Jun, \{Bong Hyun\} and Cheulhee Jung and Lee, \{Seung Hwan\}",

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year = "2022",

month = dec,

doi = "10.1186/s13059-022-02644-8",

language = "English",

volume = "23",

journal = "Genome Biology",

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T1 - Expansion of the prime editing modality with Cas9 from Francisella novicida

AU - Oh, Yeounsun

AU - Lee, Wi jae

AU - Hur, Junho K.

AU - Song, Woo Jeung

AU - Lee, Youngjeon

AU - Kim, Hanseop

AU - Gwon, Lee Wha

AU - Kim, Young Hyun

AU - Park, Young Ho

AU - Kim, Chan Hyoung

AU - Lim, Kyung Seob

AU - Song, Bong Seok

AU - Huh, Jae Won

AU - Kim, Sun Uk

AU - Jun, Bong Hyun

AU - Jung, Cheulhee

AU - Lee, Seung Hwan

PY - 2022/12

Y1 - 2022/12

N2 - Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase after nick formation by CRISPR nickase. In this study, we develop a technology that can be used to insert or replace external bases in the target DNA sequence by linking reverse transcriptase to the Francisella novicida Cas9, which is a CRISPR-Cas9 ortholog. Using FnCas9(H969A) nickase, the targeting limitation of existing Streptococcus pyogenes Cas9 nickase [SpCas9(H840A)]-based prime editing is dramatically extended, and accurate prime editing is induced specifically for the target genes in human cell lines.

AB - Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase after nick formation by CRISPR nickase. In this study, we develop a technology that can be used to insert or replace external bases in the target DNA sequence by linking reverse transcriptase to the Francisella novicida Cas9, which is a CRISPR-Cas9 ortholog. Using FnCas9(H969A) nickase, the targeting limitation of existing Streptococcus pyogenes Cas9 nickase [SpCas9(H840A)]-based prime editing is dramatically extended, and accurate prime editing is induced specifically for the target genes in human cell lines.

KW - CRISPR-Cas9

KW - Francisella novicida

KW - Ortholog

KW - Prime editing

KW - Target expansion

UR - https://www.scopus.com/pages/publications/85128054199

U2 - 10.1186/s13059-022-02644-8

DO - 10.1186/s13059-022-02644-8

M3 - Article

C2 - 35410288

AN - SCOPUS:85128054199

SN - 1474-7596

VL - 23

JO - Genome Biology

JF - Genome Biology

IS - 1

M1 - 92

ER -

Expansion of the prime editing modality with Cas9 from Francisella novicida

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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