TY - JOUR
T1 - Exposure to aflatoxin B1 attenuates cell viability and induces endoplasmic reticulum-mediated cell death in a bovine mammary epithelial cell line (MAC-T)
AU - Park, Wonhyoung
AU - Park, Min Young
AU - Song, Gwonhwa
AU - Lim, Whasun
N1 - Funding Information:
This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI17C0929 ) and the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science and ICT(MIST) , Republic of Korea (grant number. 2018R1C1B6009048 ).
Funding Information:
This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI17C0929) and the National Research Foundation of Korea(NRF) grant funded by the Ministry of Science and ICT(MIST), Republic of Korea (grant number. 2018R1C1B6009048).
PY - 2019/12
Y1 - 2019/12
N2 - Mammary gland epithelial cells play a key role in milk production in dairy cattle, which are prone to feed contamination from microorganisms, especially pathogenic fungi and their mycotoxin products. When mycotoxins enter the body, they cause a reduction in feed intake and milk production, leading to the retardation of growth and productivity in dairy cattle. Among them, aflatoxin B1 is a ubiquitous food contaminant frequently found in stored crops, which are used as animal feed, and causes many adverse health effects in domestic animals. Unfortunately, there is a lack of knowledge on the mechanisms underlying the detrimental effects of aflatoxin B1. Therefore, in this study, we found that aflatoxin B1 reduced cellular proliferation, induced apoptosis by DNA fragmentation, and disrupted intracellular homeostasis, including mitochondrial membrane potential and calcium concentration, in an immortalized bovine mammary epithelial cell line (MAC-T). Additionally, we identified aflatoxin B1-mediated cell signaling pathways associated with cell survival and the endoplasmic reticulum stress response. These results revealed that exposure to aflatoxin B1 attenuates cell viability and induces endoplasmic reticulum-mediated cell death in MAC-T cells.
AB - Mammary gland epithelial cells play a key role in milk production in dairy cattle, which are prone to feed contamination from microorganisms, especially pathogenic fungi and their mycotoxin products. When mycotoxins enter the body, they cause a reduction in feed intake and milk production, leading to the retardation of growth and productivity in dairy cattle. Among them, aflatoxin B1 is a ubiquitous food contaminant frequently found in stored crops, which are used as animal feed, and causes many adverse health effects in domestic animals. Unfortunately, there is a lack of knowledge on the mechanisms underlying the detrimental effects of aflatoxin B1. Therefore, in this study, we found that aflatoxin B1 reduced cellular proliferation, induced apoptosis by DNA fragmentation, and disrupted intracellular homeostasis, including mitochondrial membrane potential and calcium concentration, in an immortalized bovine mammary epithelial cell line (MAC-T). Additionally, we identified aflatoxin B1-mediated cell signaling pathways associated with cell survival and the endoplasmic reticulum stress response. These results revealed that exposure to aflatoxin B1 attenuates cell viability and induces endoplasmic reticulum-mediated cell death in MAC-T cells.
KW - Aflatoxin B1
KW - Cell death
KW - Endoplasmic reticulum stress
KW - Mammary gland cell
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U2 - 10.1016/j.tiv.2019.104591
DO - 10.1016/j.tiv.2019.104591
M3 - Article
C2 - 31279908
AN - SCOPUS:85068512244
SN - 0887-2333
VL - 61
JO - Toxicology in Vitro
JF - Toxicology in Vitro
M1 - 104591
ER -