Exposure to cigarette smoke via respiratory system may induce abnormal alterations of reproductive organs in female diabetic rats

Soo Min Kim; Kyung A. Hwang; Ryeo Eun Go; Jae Hyuck Sung; Dal Woong Choi; Kyung Chul Choi

doi:10.1002/tox.22652

Exposure to cigarette smoke via respiratory system may induce abnormal alterations of reproductive organs in female diabetic rats

Soo Min Kim, Kyung A. Hwang, Ryeo Eun Go, Jae Hyuck Sung, Dal Woong Choi, Kyung Chul Choi

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Cigarette smoke (CS) has harmful effects on human fertility, reproduction, and development as well as on patients suffering from metabolic diseases such as diabetes than on healthy individuals. This study was conducted to investigate the relationship between CS exposure and histological alterations of reproductive organs in female diabetic rats. We evaluated the histology of uteruses and ovaries obtained from female rats exposed to smoke from standard cigarettes for 4 weeks (28 hours a week). After CS exposure, tissue slides were made from uterine and ovarian samples and examined after hematoxylin and eosin staining. Immunohistochemistry was used for detection of matrix metallopeptidase 9 (MMP9), C-X-C chemokine receptor type 4 (CXCR4), and estrogen receptor (ER)α in the uterus and ovary. MMP9 is an inflammatory biomarker that increases during progression to endometriosis. As a chemokine receptor, CXCR4 is involved in development of the inner wall of the uterus and cell adhesion. In the uterus, the occurrence of MMP9, CXCR4, and ERα and the number of endometrial glands were increased by CS exposure, while in the ovary, occurrence of MMP9, CXCR4, ERα, proliferating cell nuclear antigen and the number of corpus lutea or cyst follicles were increased by CS exposure. Collectively, this study indicates that CS induced abnormal development of the uterus and ovary under induced diabetes, leading to adverse effects on normal function of reproductive organs in female rats. Highlights: Cigarette smoke (CS) exposure adversely affected reproductive organs of diabetic female rats. In the uterus, expression of matrix metallopeptidase 9 (MMP9), C-X-C chemokine receptor type 4 (CXCR4), estrogen receptor (ER)α, and the number of endometrial glands were increased by CS exposure, In the ovary, the expression of MMP9, CXCR4, ERα, and proliferating cell nuclear antigen and the number of corpus lutea or cyst follicles were increased by CS exposure. Exposure to CS via the respiratory system exerted a harmful impact on the uterus and ovary in female rats with diabetes.

Original language	English
Pages (from-to)	13-21
Number of pages	9
Journal	Environmental Toxicology
Volume	34
Issue number	1
DOIs	https://doi.org/10.1002/tox.22652
Publication status	Published - 2019 Jan

Bibliographical note

Funding Information:
information Ministry of Food and Drug Safety, Grant/Award Number: 14182MFDS977; Ministry of Science, ICT and Future Planning, Grant/Award Number: 2017K1A4A3014959This research was supported by a grant (14182MFDS977) from the Ministry of Food and Drug Safety in 2017. In addition, this work was supported by the Global Research and Development Center (GRDC) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2017K1A4A3014959).

Funding Information:
This research was supported by a grant (14182MFDS977) from the Ministry of Food and Drug Safety in 2017. In addition, this work was supported by the Global Research and Development Center (GRDC) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2017K1A4A3014959).

Publisher Copyright:
© 2018 Wiley Periodicals, Inc.

Keywords

cigarette smoke exposure
diabetic rats
ovarian follicle
uterus

ASJC Scopus subject areas

Toxicology
Management, Monitoring, Policy and Law
Health, Toxicology and Mutagenesis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/tox.22652

Cite this

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title = "Exposure to cigarette smoke via respiratory system may induce abnormal alterations of reproductive organs in female diabetic rats",

abstract = "Cigarette smoke (CS) has harmful effects on human fertility, reproduction, and development as well as on patients suffering from metabolic diseases such as diabetes than on healthy individuals. This study was conducted to investigate the relationship between CS exposure and histological alterations of reproductive organs in female diabetic rats. We evaluated the histology of uteruses and ovaries obtained from female rats exposed to smoke from standard cigarettes for 4 weeks (28 hours a week). After CS exposure, tissue slides were made from uterine and ovarian samples and examined after hematoxylin and eosin staining. Immunohistochemistry was used for detection of matrix metallopeptidase 9 (MMP9), C-X-C chemokine receptor type 4 (CXCR4), and estrogen receptor (ER)α in the uterus and ovary. MMP9 is an inflammatory biomarker that increases during progression to endometriosis. As a chemokine receptor, CXCR4 is involved in development of the inner wall of the uterus and cell adhesion. In the uterus, the occurrence of MMP9, CXCR4, and ERα and the number of endometrial glands were increased by CS exposure, while in the ovary, occurrence of MMP9, CXCR4, ERα, proliferating cell nuclear antigen and the number of corpus lutea or cyst follicles were increased by CS exposure. Collectively, this study indicates that CS induced abnormal development of the uterus and ovary under induced diabetes, leading to adverse effects on normal function of reproductive organs in female rats. Highlights: Cigarette smoke (CS) exposure adversely affected reproductive organs of diabetic female rats. In the uterus, expression of matrix metallopeptidase 9 (MMP9), C-X-C chemokine receptor type 4 (CXCR4), estrogen receptor (ER)α, and the number of endometrial glands were increased by CS exposure, In the ovary, the expression of MMP9, CXCR4, ERα, and proliferating cell nuclear antigen and the number of corpus lutea or cyst follicles were increased by CS exposure. Exposure to CS via the respiratory system exerted a harmful impact on the uterus and ovary in female rats with diabetes.",

keywords = "cigarette smoke exposure, diabetic rats, ovarian follicle, uterus",

author = "Kim, {Soo Min} and Hwang, {Kyung A.} and Go, {Ryeo Eun} and Sung, {Jae Hyuck} and Choi, {Dal Woong} and Choi, {Kyung Chul}",

note = "Funding Information: information Ministry of Food and Drug Safety, Grant/Award Number: 14182MFDS977; Ministry of Science, ICT and Future Planning, Grant/Award Number: 2017K1A4A3014959This research was supported by a grant (14182MFDS977) from the Ministry of Food and Drug Safety in 2017. In addition, this work was supported by the Global Research and Development Center (GRDC) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2017K1A4A3014959). Funding Information: This research was supported by a grant (14182MFDS977) from the Ministry of Food and Drug Safety in 2017. In addition, this work was supported by the Global Research and Development Center (GRDC) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2017K1A4A3014959). Publisher Copyright: {\textcopyright} 2018 Wiley Periodicals, Inc.",

year = "2019",

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doi = "10.1002/tox.22652",

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AU - Kim, Soo Min

AU - Hwang, Kyung A.

AU - Go, Ryeo Eun

AU - Sung, Jae Hyuck

AU - Choi, Dal Woong

AU - Choi, Kyung Chul

N1 - Funding Information: information Ministry of Food and Drug Safety, Grant/Award Number: 14182MFDS977; Ministry of Science, ICT and Future Planning, Grant/Award Number: 2017K1A4A3014959This research was supported by a grant (14182MFDS977) from the Ministry of Food and Drug Safety in 2017. In addition, this work was supported by the Global Research and Development Center (GRDC) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2017K1A4A3014959). Funding Information: This research was supported by a grant (14182MFDS977) from the Ministry of Food and Drug Safety in 2017. In addition, this work was supported by the Global Research and Development Center (GRDC) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2017K1A4A3014959). Publisher Copyright: © 2018 Wiley Periodicals, Inc.

PY - 2019/1

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N2 - Cigarette smoke (CS) has harmful effects on human fertility, reproduction, and development as well as on patients suffering from metabolic diseases such as diabetes than on healthy individuals. This study was conducted to investigate the relationship between CS exposure and histological alterations of reproductive organs in female diabetic rats. We evaluated the histology of uteruses and ovaries obtained from female rats exposed to smoke from standard cigarettes for 4 weeks (28 hours a week). After CS exposure, tissue slides were made from uterine and ovarian samples and examined after hematoxylin and eosin staining. Immunohistochemistry was used for detection of matrix metallopeptidase 9 (MMP9), C-X-C chemokine receptor type 4 (CXCR4), and estrogen receptor (ER)α in the uterus and ovary. MMP9 is an inflammatory biomarker that increases during progression to endometriosis. As a chemokine receptor, CXCR4 is involved in development of the inner wall of the uterus and cell adhesion. In the uterus, the occurrence of MMP9, CXCR4, and ERα and the number of endometrial glands were increased by CS exposure, while in the ovary, occurrence of MMP9, CXCR4, ERα, proliferating cell nuclear antigen and the number of corpus lutea or cyst follicles were increased by CS exposure. Collectively, this study indicates that CS induced abnormal development of the uterus and ovary under induced diabetes, leading to adverse effects on normal function of reproductive organs in female rats. Highlights: Cigarette smoke (CS) exposure adversely affected reproductive organs of diabetic female rats. In the uterus, expression of matrix metallopeptidase 9 (MMP9), C-X-C chemokine receptor type 4 (CXCR4), estrogen receptor (ER)α, and the number of endometrial glands were increased by CS exposure, In the ovary, the expression of MMP9, CXCR4, ERα, and proliferating cell nuclear antigen and the number of corpus lutea or cyst follicles were increased by CS exposure. Exposure to CS via the respiratory system exerted a harmful impact on the uterus and ovary in female rats with diabetes.

AB - Cigarette smoke (CS) has harmful effects on human fertility, reproduction, and development as well as on patients suffering from metabolic diseases such as diabetes than on healthy individuals. This study was conducted to investigate the relationship between CS exposure and histological alterations of reproductive organs in female diabetic rats. We evaluated the histology of uteruses and ovaries obtained from female rats exposed to smoke from standard cigarettes for 4 weeks (28 hours a week). After CS exposure, tissue slides were made from uterine and ovarian samples and examined after hematoxylin and eosin staining. Immunohistochemistry was used for detection of matrix metallopeptidase 9 (MMP9), C-X-C chemokine receptor type 4 (CXCR4), and estrogen receptor (ER)α in the uterus and ovary. MMP9 is an inflammatory biomarker that increases during progression to endometriosis. As a chemokine receptor, CXCR4 is involved in development of the inner wall of the uterus and cell adhesion. In the uterus, the occurrence of MMP9, CXCR4, and ERα and the number of endometrial glands were increased by CS exposure, while in the ovary, occurrence of MMP9, CXCR4, ERα, proliferating cell nuclear antigen and the number of corpus lutea or cyst follicles were increased by CS exposure. Collectively, this study indicates that CS induced abnormal development of the uterus and ovary under induced diabetes, leading to adverse effects on normal function of reproductive organs in female rats. Highlights: Cigarette smoke (CS) exposure adversely affected reproductive organs of diabetic female rats. In the uterus, expression of matrix metallopeptidase 9 (MMP9), C-X-C chemokine receptor type 4 (CXCR4), estrogen receptor (ER)α, and the number of endometrial glands were increased by CS exposure, In the ovary, the expression of MMP9, CXCR4, ERα, and proliferating cell nuclear antigen and the number of corpus lutea or cyst follicles were increased by CS exposure. Exposure to CS via the respiratory system exerted a harmful impact on the uterus and ovary in female rats with diabetes.

KW - cigarette smoke exposure

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Exposure to cigarette smoke via respiratory system may induce abnormal alterations of reproductive organs in female diabetic rats

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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