We examined ischemia-related changes of calbindin D-28k (CB) immunoreactivity in L 7 of the spinal ventral horn after transient spinal cord ischemia in rabbits. In the sham-operated group, CB immunoreactivity was not present in the spinal ventral horn, but CB immunoreactivity was detectable in the dorsal horn. CB immunoreactivity was detectable in the ventral horn at 30 min after ischemia: the CB immunoreactivity was found in glial cells identified as astrocytes. At 1 h after ischemia, CB immunoreactivity was highest and present at a few somata located in the lamina VII as well as many glial cells. CB immunoreactivity was lower in the lamina VII at 3 h after ischemia compared to 1 h post-ischemic group. By 2 days after ischemia, CB immunoreactivity was decreased in this region. In addition, the result of Western blot result showed the pattern of CB expression similar to that of immunohistochemistry. In conclusion, the ischemia-related changes of CB immunoreactivity in neurons and glial cells in the ischemic spinal ventral horn in rabbits may be related to modulation of intracellular calcium following transient ischemia.
Bibliographical noteFunding Information:
The authors would like to thank Mr. Suek Han, Seung Jin Jeon and Seung Uk Lee for their technical help during this study. This work was supported by the Research Grant from Hallym University, Korea.
- Calbindin D-28k
- Calcium-binding proteins
- Spinal cord ischemia
ASJC Scopus subject areas
- General Neuroscience