Expression and changes of calbindin D-28k immunoreactivity in the ventral horn after transient spinal cord ischemia in rabbits

Jae Chul Lee; In Koo Hwang; Jun Hwi Cho; Seung Myung Moon; Tae Cheon Kang; Won Ki Kim; Moo Ho Won

doi:10.1016/j.neulet.2004.07.082

Expression and changes of calbindin D-28k immunoreactivity in the ventral horn after transient spinal cord ischemia in rabbits

Jae Chul Lee, In Koo Hwang, Jun Hwi Cho, Seung Myung Moon, Tae Cheon Kang, Won Ki Kim, Moo Ho Won

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

We examined ischemia-related changes of calbindin D-28k (CB) immunoreactivity in L ₇ of the spinal ventral horn after transient spinal cord ischemia in rabbits. In the sham-operated group, CB immunoreactivity was not present in the spinal ventral horn, but CB immunoreactivity was detectable in the dorsal horn. CB immunoreactivity was detectable in the ventral horn at 30 min after ischemia: the CB immunoreactivity was found in glial cells identified as astrocytes. At 1 h after ischemia, CB immunoreactivity was highest and present at a few somata located in the lamina VII as well as many glial cells. CB immunoreactivity was lower in the lamina VII at 3 h after ischemia compared to 1 h post-ischemic group. By 2 days after ischemia, CB immunoreactivity was decreased in this region. In addition, the result of Western blot result showed the pattern of CB expression similar to that of immunohistochemistry. In conclusion, the ischemia-related changes of CB immunoreactivity in neurons and glial cells in the ischemic spinal ventral horn in rabbits may be related to modulation of intracellular calcium following transient ischemia.

Original language	English
Pages (from-to)	145-149
Number of pages	5
Journal	Neuroscience Letters
Volume	369
Issue number	2
DOIs	https://doi.org/10.1016/j.neulet.2004.07.082
Publication status	Published - 2004 Oct 14
Externally published	Yes

Bibliographical note

Funding Information:
The authors would like to thank Mr. Suek Han, Seung Jin Jeon and Seung Uk Lee for their technical help during this study. This work was supported by the Research Grant from Hallym University, Korea.

Keywords

Astrocytes
Calbindin D-28k
Calcium-binding proteins
Excitotoxicity
Interneurons
Rabbit
Spinal cord ischemia

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neulet.2004.07.082

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title = "Expression and changes of calbindin D-28k immunoreactivity in the ventral horn after transient spinal cord ischemia in rabbits",

abstract = "We examined ischemia-related changes of calbindin D-28k (CB) immunoreactivity in L 7 of the spinal ventral horn after transient spinal cord ischemia in rabbits. In the sham-operated group, CB immunoreactivity was not present in the spinal ventral horn, but CB immunoreactivity was detectable in the dorsal horn. CB immunoreactivity was detectable in the ventral horn at 30 min after ischemia: the CB immunoreactivity was found in glial cells identified as astrocytes. At 1 h after ischemia, CB immunoreactivity was highest and present at a few somata located in the lamina VII as well as many glial cells. CB immunoreactivity was lower in the lamina VII at 3 h after ischemia compared to 1 h post-ischemic group. By 2 days after ischemia, CB immunoreactivity was decreased in this region. In addition, the result of Western blot result showed the pattern of CB expression similar to that of immunohistochemistry. In conclusion, the ischemia-related changes of CB immunoreactivity in neurons and glial cells in the ischemic spinal ventral horn in rabbits may be related to modulation of intracellular calcium following transient ischemia.",

keywords = "Astrocytes, Calbindin D-28k, Calcium-binding proteins, Excitotoxicity, Interneurons, Rabbit, Spinal cord ischemia",

author = "Lee, {Jae Chul} and Hwang, {In Koo} and Cho, {Jun Hwi} and Moon, {Seung Myung} and Kang, {Tae Cheon} and Kim, {Won Ki} and Won, {Moo Ho}",

note = "Funding Information: The authors would like to thank Mr. Suek Han, Seung Jin Jeon and Seung Uk Lee for their technical help during this study. This work was supported by the Research Grant from Hallym University, Korea.",

year = "2004",

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doi = "10.1016/j.neulet.2004.07.082",

language = "English",

volume = "369",

pages = "145--149",

journal = "Neuroscience Letters",

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T1 - Expression and changes of calbindin D-28k immunoreactivity in the ventral horn after transient spinal cord ischemia in rabbits

AU - Lee, Jae Chul

AU - Hwang, In Koo

AU - Cho, Jun Hwi

AU - Moon, Seung Myung

AU - Kang, Tae Cheon

AU - Kim, Won Ki

AU - Won, Moo Ho

N1 - Funding Information: The authors would like to thank Mr. Suek Han, Seung Jin Jeon and Seung Uk Lee for their technical help during this study. This work was supported by the Research Grant from Hallym University, Korea.

PY - 2004/10/14

Y1 - 2004/10/14

N2 - We examined ischemia-related changes of calbindin D-28k (CB) immunoreactivity in L 7 of the spinal ventral horn after transient spinal cord ischemia in rabbits. In the sham-operated group, CB immunoreactivity was not present in the spinal ventral horn, but CB immunoreactivity was detectable in the dorsal horn. CB immunoreactivity was detectable in the ventral horn at 30 min after ischemia: the CB immunoreactivity was found in glial cells identified as astrocytes. At 1 h after ischemia, CB immunoreactivity was highest and present at a few somata located in the lamina VII as well as many glial cells. CB immunoreactivity was lower in the lamina VII at 3 h after ischemia compared to 1 h post-ischemic group. By 2 days after ischemia, CB immunoreactivity was decreased in this region. In addition, the result of Western blot result showed the pattern of CB expression similar to that of immunohistochemistry. In conclusion, the ischemia-related changes of CB immunoreactivity in neurons and glial cells in the ischemic spinal ventral horn in rabbits may be related to modulation of intracellular calcium following transient ischemia.

AB - We examined ischemia-related changes of calbindin D-28k (CB) immunoreactivity in L 7 of the spinal ventral horn after transient spinal cord ischemia in rabbits. In the sham-operated group, CB immunoreactivity was not present in the spinal ventral horn, but CB immunoreactivity was detectable in the dorsal horn. CB immunoreactivity was detectable in the ventral horn at 30 min after ischemia: the CB immunoreactivity was found in glial cells identified as astrocytes. At 1 h after ischemia, CB immunoreactivity was highest and present at a few somata located in the lamina VII as well as many glial cells. CB immunoreactivity was lower in the lamina VII at 3 h after ischemia compared to 1 h post-ischemic group. By 2 days after ischemia, CB immunoreactivity was decreased in this region. In addition, the result of Western blot result showed the pattern of CB expression similar to that of immunohistochemistry. In conclusion, the ischemia-related changes of CB immunoreactivity in neurons and glial cells in the ischemic spinal ventral horn in rabbits may be related to modulation of intracellular calcium following transient ischemia.

KW - Astrocytes

KW - Calbindin D-28k

KW - Calcium-binding proteins

KW - Excitotoxicity

KW - Interneurons

KW - Rabbit

KW - Spinal cord ischemia

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Expression and changes of calbindin D-28k immunoreactivity in the ventral horn after transient spinal cord ischemia in rabbits

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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