Expression of human interleukin-2 from native and synthetic genes in E. coli: No correlation between major codon bias and high level expression

Hee Won Lee; Jae Hoon Joo; Seongman Kang; In Sung Song; Jong Bum Kwon; Moon H. Han; Doe Sun Na

doi:10.1007/BF01021637

Expression of human interleukin-2 from native and synthetic genes in E. coli: No correlation between major codon bias and high level expression

Hee Won Lee, Jae Hoon Joo, Seongman Kang, In Sung Song, Jong Bum Kwon, Moon H. Han, Doe Sun Na

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Expression of human IL-2 from the cDNA and synthetic gene was compared using the E. coli expression system. Expression plasmids containing the cDNA or synthetic gene in which the only difference was the coding sequences were constructed. Expression of IL-2 from the cDNA and synthetic gene were estimated to be as high as 18% and 14%, respectively. No correlation was found between the major codon bias and high level expression of IL-2 in this system.

Original language	English
Pages (from-to)	653-658
Number of pages	6
Journal	Biotechnology letters
Volume	14
Issue number	8
DOIs	https://doi.org/10.1007/BF01021637
Publication status	Published - 1992 Aug

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/BF01021637

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title = "Expression of human interleukin-2 from native and synthetic genes in E. coli: No correlation between major codon bias and high level expression",

abstract = "Expression of human IL-2 from the cDNA and synthetic gene was compared using the E. coli expression system. Expression plasmids containing the cDNA or synthetic gene in which the only difference was the coding sequences were constructed. Expression of IL-2 from the cDNA and synthetic gene were estimated to be as high as 18% and 14%, respectively. No correlation was found between the major codon bias and high level expression of IL-2 in this system.",

author = "Lee, {Hee Won} and Joo, {Jae Hoon} and Seongman Kang and Song, {In Sung} and Kwon, {Jong Bum} and Han, {Moon H.} and Na, {Doe Sun}",

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journal = "Biotechnology letters",

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T1 - Expression of human interleukin-2 from native and synthetic genes in E. coli

T2 - No correlation between major codon bias and high level expression

AU - Lee, Hee Won

AU - Joo, Jae Hoon

AU - Kang, Seongman

AU - Song, In Sung

AU - Kwon, Jong Bum

AU - Han, Moon H.

AU - Na, Doe Sun

PY - 1992/8

Y1 - 1992/8

N2 - Expression of human IL-2 from the cDNA and synthetic gene was compared using the E. coli expression system. Expression plasmids containing the cDNA or synthetic gene in which the only difference was the coding sequences were constructed. Expression of IL-2 from the cDNA and synthetic gene were estimated to be as high as 18% and 14%, respectively. No correlation was found between the major codon bias and high level expression of IL-2 in this system.

AB - Expression of human IL-2 from the cDNA and synthetic gene was compared using the E. coli expression system. Expression plasmids containing the cDNA or synthetic gene in which the only difference was the coding sequences were constructed. Expression of IL-2 from the cDNA and synthetic gene were estimated to be as high as 18% and 14%, respectively. No correlation was found between the major codon bias and high level expression of IL-2 in this system.

UR - http://www.scopus.com/inward/record.url?scp=0026646014&partnerID=8YFLogxK

U2 - 10.1007/BF01021637

DO - 10.1007/BF01021637

M3 - Article

AN - SCOPUS:0026646014

SN - 0141-5492

VL - 14

SP - 653

EP - 658

JO - Biotechnology letters

JF - Biotechnology letters

IS - 8

ER -

Expression of human interleukin-2 from native and synthetic genes in E. coli: No correlation between major codon bias and high level expression

Abstract

ASJC Scopus subject areas

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