Expression of human interleukin-2 from native and synthetic genes in E. coli: No correlation between major codon bias and high level expression

Hee Won Lee, Jae Hoon Joo, Seongman Kang, In Sung Song, Jong Bum Kwon, Moon H. Han, Doe Sun Na

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    Expression of human IL-2 from the cDNA and synthetic gene was compared using the E. coli expression system. Expression plasmids containing the cDNA or synthetic gene in which the only difference was the coding sequences were constructed. Expression of IL-2 from the cDNA and synthetic gene were estimated to be as high as 18% and 14%, respectively. No correlation was found between the major codon bias and high level expression of IL-2 in this system.

    Original languageEnglish
    Pages (from-to)653-658
    Number of pages6
    JournalBiotechnology letters
    Volume14
    Issue number8
    DOIs
    Publication statusPublished - 1992 Aug

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

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