Expression of Major Histocompatibility Complex during Neuronal Differentiation of Somatic Cell Nuclear Transfer-Human Embryonic Stem Cells

Jin Saem Lee, Jeoung Eun Lee, Shin Hye Yu, Taehoon Chun, Mi Yoon Chang, Dong Ryul Lee, Chang Hwan Park

Research output: Contribution to journalArticlepeer-review

Abstract

Human pluripotent stem cells (hPSCs) such as human embryonic stem cells (hESCs), induced pluripotent stem cells, and somatic cell nuclear transfer (SCNT)-hESCs can permanently self-renew while maintaining their capacity to differentiate into any type of somatic cells, thereby serving as an important cell source for cell therapy. However, there are persistent challenges in the application of hPSCs in clinical trials, where one of the most significant is graft rejection by the patient immune system in response to human leukocyte antigen (HLA) mismatch when transplants are obtained from an allogeneic (non-self) cell source. Homozygous SCNT-hESCs (homo-SCNT-hESCs) were used to simplify the clinical appli cation and to reduce HLA mismatch. Here, we present a xeno-free protocol that confirms the efficient generation of neural precursor cells in hPSCs and also the differentiation of dopaminergic neurons. Additionally, there was no differ ence when comparing the HLA expression patterns of hESC, homo-SCNT-hESCs and hetero-SCNT-hESCs. We pro pose that there are no differences in the differentiation capacity and HLA expression among hPSCs that can be cultured in vitro. Thus, it is expected that homo-SCNT-hESCs will possess a wider range of applications when transplanted with neural precursor cells in the context of clinical trials.

Original languageEnglish
Pages (from-to)59-69
Number of pages11
JournalInternational Journal of Stem Cells
Volume17
Issue number1
DOIs
Publication statusPublished - 2024

Bibliographical note

Publisher Copyright:
© 2024 by the Korean Society for Stem Cell Research

Keywords

  • Dopaminergic differentiation
  • HLA expression
  • Human SCNT ESC
  • Neuronal differentiation

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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