Expression profiling of Streptomyces peucetius metabolic genes using DNA microarray analysis

Joon Ryeol Yang; Eunjung Song; Byung Gee Kim; Eung Soo Kim; Jae Kyung Sohng; Min Kyu Oh

doi:10.1007/s12257-008-0114-4

Expression profiling of Streptomyces peucetius metabolic genes using DNA microarray analysis

Joon Ryeol Yang, Eunjung Song, Byung Gee Kim, Eung Soo Kim, Jae Kyung Sohng, Min Kyu Oh

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Doxorubicin (DXR) and daunorubicin (DNR) are anthracycline antibiotics produced by Streptomyces peucetius and widely used as cancer chemotherapeutic agents. To improve their productivity, regulation of DXR/DNR synthesis genes as well as central metabolic pathway genes must be understood more clearly. So far, studies have focused on DXR/DNR gene regulation. To investigate the correlation between the central metabolic pathway genes and DXR/DNR productivity, we selected 265 genes involved in glycolysis, fermentation, the citric acid cycle, butanoate metabolism, etc., and searched for their sequences in the S. peucetius genome by comparing gene sequences to those of Streptomyces coelicolor. The homologous genes were amplified by PCR and arrayed on glass microarray slides. Gene expression was monitored under two different growth media conditions, R2YE and NDYE. Genes involved in the production of malonyl-CoA and propionyl-CoA, the main precursors for doxorubicin synthesis, were mainly upregulated in NDYE media. Genes related to acetyl-CoA and the urea cycle were also upregulated. These changes in gene expression were confirmed by real-time RT-PCR.

Original language	English
Pages (from-to)	738-744
Number of pages	7
Journal	Biotechnology and Bioprocess Engineering
Volume	13
Issue number	6
DOIs	https://doi.org/10.1007/s12257-008-0114-4
Publication status	Published - 2008

Bibliographical note

Funding Information:
Kim at the Korea University medical school for substantial help in DNA microarray preparation. This work was supported by the Korea Research Foundation Grant funded by the Korean Government (KRF-2006-005-J03603). This work was also partially supported by the Korean Systems Biology Program from the Ministry of Education, Science and Technology through the Korea Science and Engineering Foundation (M10309020000-03B5002-00000, to E.-S. Kim).

Keywords

DNA microarray
Doxorubicin
Metabolic gene
Streptomyces peucetius

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Biomedical Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s12257-008-0114-4

Cite this

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title = "Expression profiling of Streptomyces peucetius metabolic genes using DNA microarray analysis",

abstract = "Doxorubicin (DXR) and daunorubicin (DNR) are anthracycline antibiotics produced by Streptomyces peucetius and widely used as cancer chemotherapeutic agents. To improve their productivity, regulation of DXR/DNR synthesis genes as well as central metabolic pathway genes must be understood more clearly. So far, studies have focused on DXR/DNR gene regulation. To investigate the correlation between the central metabolic pathway genes and DXR/DNR productivity, we selected 265 genes involved in glycolysis, fermentation, the citric acid cycle, butanoate metabolism, etc., and searched for their sequences in the S. peucetius genome by comparing gene sequences to those of Streptomyces coelicolor. The homologous genes were amplified by PCR and arrayed on glass microarray slides. Gene expression was monitored under two different growth media conditions, R2YE and NDYE. Genes involved in the production of malonyl-CoA and propionyl-CoA, the main precursors for doxorubicin synthesis, were mainly upregulated in NDYE media. Genes related to acetyl-CoA and the urea cycle were also upregulated. These changes in gene expression were confirmed by real-time RT-PCR.",

keywords = "DNA microarray, Doxorubicin, Metabolic gene, Streptomyces peucetius",

author = "Yang, {Joon Ryeol} and Eunjung Song and Kim, {Byung Gee} and Kim, {Eung Soo} and Sohng, {Jae Kyung} and Oh, {Min Kyu}",

note = "Funding Information: Kim at the Korea University medical school for substantial help in DNA microarray preparation. This work was supported by the Korea Research Foundation Grant funded by the Korean Government (KRF-2006-005-J03603). This work was also partially supported by the Korean Systems Biology Program from the Ministry of Education, Science and Technology through the Korea Science and Engineering Foundation (M10309020000-03B5002-00000, to E.-S. Kim).",

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doi = "10.1007/s12257-008-0114-4",

language = "English",

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AU - Yang, Joon Ryeol

AU - Song, Eunjung

AU - Kim, Byung Gee

AU - Kim, Eung Soo

AU - Sohng, Jae Kyung

AU - Oh, Min Kyu

N1 - Funding Information: Kim at the Korea University medical school for substantial help in DNA microarray preparation. This work was supported by the Korea Research Foundation Grant funded by the Korean Government (KRF-2006-005-J03603). This work was also partially supported by the Korean Systems Biology Program from the Ministry of Education, Science and Technology through the Korea Science and Engineering Foundation (M10309020000-03B5002-00000, to E.-S. Kim).

PY - 2008

Y1 - 2008

N2 - Doxorubicin (DXR) and daunorubicin (DNR) are anthracycline antibiotics produced by Streptomyces peucetius and widely used as cancer chemotherapeutic agents. To improve their productivity, regulation of DXR/DNR synthesis genes as well as central metabolic pathway genes must be understood more clearly. So far, studies have focused on DXR/DNR gene regulation. To investigate the correlation between the central metabolic pathway genes and DXR/DNR productivity, we selected 265 genes involved in glycolysis, fermentation, the citric acid cycle, butanoate metabolism, etc., and searched for their sequences in the S. peucetius genome by comparing gene sequences to those of Streptomyces coelicolor. The homologous genes were amplified by PCR and arrayed on glass microarray slides. Gene expression was monitored under two different growth media conditions, R2YE and NDYE. Genes involved in the production of malonyl-CoA and propionyl-CoA, the main precursors for doxorubicin synthesis, were mainly upregulated in NDYE media. Genes related to acetyl-CoA and the urea cycle were also upregulated. These changes in gene expression were confirmed by real-time RT-PCR.

AB - Doxorubicin (DXR) and daunorubicin (DNR) are anthracycline antibiotics produced by Streptomyces peucetius and widely used as cancer chemotherapeutic agents. To improve their productivity, regulation of DXR/DNR synthesis genes as well as central metabolic pathway genes must be understood more clearly. So far, studies have focused on DXR/DNR gene regulation. To investigate the correlation between the central metabolic pathway genes and DXR/DNR productivity, we selected 265 genes involved in glycolysis, fermentation, the citric acid cycle, butanoate metabolism, etc., and searched for their sequences in the S. peucetius genome by comparing gene sequences to those of Streptomyces coelicolor. The homologous genes were amplified by PCR and arrayed on glass microarray slides. Gene expression was monitored under two different growth media conditions, R2YE and NDYE. Genes involved in the production of malonyl-CoA and propionyl-CoA, the main precursors for doxorubicin synthesis, were mainly upregulated in NDYE media. Genes related to acetyl-CoA and the urea cycle were also upregulated. These changes in gene expression were confirmed by real-time RT-PCR.

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Expression profiling of Streptomyces peucetius metabolic genes using DNA microarray analysis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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