Extreme furfural tolerance of a soil bacterium Enterobacter cloacae GGT036

Sun Young Choi, Gyeongtaek Gong, Hong Sil Park, Youngsoon Um, Sang Jun Sim, Han Min Woo

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12 Citations (Scopus)


Detoxification process of cellular inhibitors including furfural is essential for production of bio-based chemicals from lignocellulosic biomass. Here we isolated an extreme furfural-tolerant bacterium Enterobacter cloacae GGT036 from soil sample collected in Mt. Gwanak, Republic of Korea. Among isolated bacteria, only E. cloacae GGT036 showed cell growth with 35mM furfural under aerobic culture. Compared to the maximal half inhibitory concentration (IC50) of well-known industrial strains Escherichia coli (24.9mM furfural) and Corynebacterium glutamicum (10mM furfural) based on the cell density, IC50 of E. cloacae GGT036 (47.7mM) was significantly higher after 24h, compared to E. coli and C. glutamicum. Since bacterial cell growth was exponentially inhibited depending on linearly increased furfural concentrations in the medium, we concluded that E. cloacae GGT036 is an extreme furfural-tolerant bacterium. Recently, the complete genome sequence of E. cloacae GGT036 was announced and this could provide an insight for engineering of E. cloacae GGT036 itself or other industrially relevant bacteria.

Original languageEnglish
Pages (from-to)11-13
Number of pages3
JournalJournal of Biotechnology
Publication statusPublished - 2015 Jan 1

Bibliographical note

Funding Information:
This work was supported by Creative Allied Program (CAP) of the Korea Research Council of Fundamental Science and Technology (KRCF)/ Korea Institute of Science and Technology (KIST) (Project No. 2E24832) and Korea CCS R&D Center (KCRC) (no. 2014M1A8A1049277 ) and the National Research Foundation of Kore a grant-funded by the Korean Government (Ministry of Science, ICT & Future Planning) (2014, University-Institute Cooperation program).

Publisher Copyright:
© 2014 Elsevier B.V.


  • Detoxification
  • Enterobacter cloacae
  • Furfural tolerance
  • IC

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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