Extreme furfural tolerance of a soil bacterium Enterobacter cloacae GGT036

Sun Young Choi; Gyeongtaek Gong; Hong Sil Park; Youngsoon Um; Sang Jun Sim; Han Min Woo

doi:10.1016/j.jbiotec.2014.11.001

Extreme furfural tolerance of a soil bacterium Enterobacter cloacae GGT036

Sun Young Choi, Gyeongtaek Gong, Hong Sil Park, Youngsoon Um, Sang Jun Sim, Han Min Woo

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Detoxification process of cellular inhibitors including furfural is essential for production of bio-based chemicals from lignocellulosic biomass. Here we isolated an extreme furfural-tolerant bacterium Enterobacter cloacae GGT036 from soil sample collected in Mt. Gwanak, Republic of Korea. Among isolated bacteria, only E. cloacae GGT036 showed cell growth with 35mM furfural under aerobic culture. Compared to the maximal half inhibitory concentration (IC₅₀) of well-known industrial strains Escherichia coli (24.9mM furfural) and Corynebacterium glutamicum (10mM furfural) based on the cell density, IC₅₀ of E. cloacae GGT036 (47.7mM) was significantly higher after 24h, compared to E. coli and C. glutamicum. Since bacterial cell growth was exponentially inhibited depending on linearly increased furfural concentrations in the medium, we concluded that E. cloacae GGT036 is an extreme furfural-tolerant bacterium. Recently, the complete genome sequence of E. cloacae GGT036 was announced and this could provide an insight for engineering of E. cloacae GGT036 itself or other industrially relevant bacteria.

Original language	English
Pages (from-to)	11-13
Number of pages	3
Journal	Journal of Biotechnology
Volume	193
DOIs	https://doi.org/10.1016/j.jbiotec.2014.11.001
Publication status	Published - 2015 Jan 1

Keywords

Detoxification
Enterobacter cloacae
Furfural tolerance
IC

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.jbiotec.2014.11.001

Cite this

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title = "Extreme furfural tolerance of a soil bacterium Enterobacter cloacae GGT036",

abstract = "Detoxification process of cellular inhibitors including furfural is essential for production of bio-based chemicals from lignocellulosic biomass. Here we isolated an extreme furfural-tolerant bacterium Enterobacter cloacae GGT036 from soil sample collected in Mt. Gwanak, Republic of Korea. Among isolated bacteria, only E. cloacae GGT036 showed cell growth with 35mM furfural under aerobic culture. Compared to the maximal half inhibitory concentration (IC50) of well-known industrial strains Escherichia coli (24.9mM furfural) and Corynebacterium glutamicum (10mM furfural) based on the cell density, IC50 of E. cloacae GGT036 (47.7mM) was significantly higher after 24h, compared to E. coli and C. glutamicum. Since bacterial cell growth was exponentially inhibited depending on linearly increased furfural concentrations in the medium, we concluded that E. cloacae GGT036 is an extreme furfural-tolerant bacterium. Recently, the complete genome sequence of E. cloacae GGT036 was announced and this could provide an insight for engineering of E. cloacae GGT036 itself or other industrially relevant bacteria.",

keywords = "Detoxification, Enterobacter cloacae, Furfural tolerance, IC",

author = "Choi, {Sun Young} and Gyeongtaek Gong and Park, {Hong Sil} and Youngsoon Um and Sim, {Sang Jun} and Woo, {Han Min}",

note = "Funding Information: This work was supported by Creative Allied Program (CAP) of the Korea Research Council of Fundamental Science and Technology (KRCF)/ Korea Institute of Science and Technology (KIST) (Project No. 2E24832) and Korea CCS R&D Center (KCRC) (no. 2014M1A8A1049277 ) and the National Research Foundation of Kore a grant-funded by the Korean Government (Ministry of Science, ICT & Future Planning) (2014, University-Institute Cooperation program). Publisher Copyright: {\textcopyright} 2014 Elsevier B.V.",

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doi = "10.1016/j.jbiotec.2014.11.001",

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AU - Choi, Sun Young

AU - Gong, Gyeongtaek

AU - Park, Hong Sil

AU - Um, Youngsoon

AU - Sim, Sang Jun

AU - Woo, Han Min

N1 - Funding Information: This work was supported by Creative Allied Program (CAP) of the Korea Research Council of Fundamental Science and Technology (KRCF)/ Korea Institute of Science and Technology (KIST) (Project No. 2E24832) and Korea CCS R&D Center (KCRC) (no. 2014M1A8A1049277 ) and the National Research Foundation of Kore a grant-funded by the Korean Government (Ministry of Science, ICT & Future Planning) (2014, University-Institute Cooperation program). Publisher Copyright: © 2014 Elsevier B.V.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Detoxification process of cellular inhibitors including furfural is essential for production of bio-based chemicals from lignocellulosic biomass. Here we isolated an extreme furfural-tolerant bacterium Enterobacter cloacae GGT036 from soil sample collected in Mt. Gwanak, Republic of Korea. Among isolated bacteria, only E. cloacae GGT036 showed cell growth with 35mM furfural under aerobic culture. Compared to the maximal half inhibitory concentration (IC50) of well-known industrial strains Escherichia coli (24.9mM furfural) and Corynebacterium glutamicum (10mM furfural) based on the cell density, IC50 of E. cloacae GGT036 (47.7mM) was significantly higher after 24h, compared to E. coli and C. glutamicum. Since bacterial cell growth was exponentially inhibited depending on linearly increased furfural concentrations in the medium, we concluded that E. cloacae GGT036 is an extreme furfural-tolerant bacterium. Recently, the complete genome sequence of E. cloacae GGT036 was announced and this could provide an insight for engineering of E. cloacae GGT036 itself or other industrially relevant bacteria.

AB - Detoxification process of cellular inhibitors including furfural is essential for production of bio-based chemicals from lignocellulosic biomass. Here we isolated an extreme furfural-tolerant bacterium Enterobacter cloacae GGT036 from soil sample collected in Mt. Gwanak, Republic of Korea. Among isolated bacteria, only E. cloacae GGT036 showed cell growth with 35mM furfural under aerobic culture. Compared to the maximal half inhibitory concentration (IC50) of well-known industrial strains Escherichia coli (24.9mM furfural) and Corynebacterium glutamicum (10mM furfural) based on the cell density, IC50 of E. cloacae GGT036 (47.7mM) was significantly higher after 24h, compared to E. coli and C. glutamicum. Since bacterial cell growth was exponentially inhibited depending on linearly increased furfural concentrations in the medium, we concluded that E. cloacae GGT036 is an extreme furfural-tolerant bacterium. Recently, the complete genome sequence of E. cloacae GGT036 was announced and this could provide an insight for engineering of E. cloacae GGT036 itself or other industrially relevant bacteria.

KW - Detoxification

KW - Enterobacter cloacae

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JF - Journal of Biotechnology

ER -

Extreme furfural tolerance of a soil bacterium Enterobacter cloacae GGT036

Abstract

Keywords

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