Fabrication and characterization of silicon-based photodiode for detection of luminol chemiluminescence in a biosensor

Hyung Il Kim; Chul Goo Kang; Moon Sik Kang; Jung Ho Park; Nam Ki Min

Fabrication and characterization of silicon-based photodiode for detection of luminol chemiluminescence in a biosensor

Hyung Il Kim, Chul Goo Kang, Moon Sik Kang, Jung Ho Park, Nam Ki Min

Research output: Contribution to journal › Conference article › peer-review

Abstract

In this paper, a silicon-based p⁺-n junction photodiode is fabricated by semiconductor processing as a detector for luminol chemiluminescence (CL) that is used in present the biosensor. The optimum conditions of the CL reaction are determined by horse-radish peroxidase (HRP) concentration, the reagent mixture ratio of luminol with HRP and hydrogen peroxide (H₂O₂) in the reactor well made of polydimethylsiloxane (PDMS). The output current of the photodiode is measured for CL by H₂O₂ concentration under 2 mM luminol and 25 units HRP. As the H₂O₂ concentration increases, the photodiode output current peak values increase. The photocurrent is linearly related to the H₂O₂ concentration in the range 0.01 ∼ 0.5 mM, and then the sensitivity of the photodiode is 23.429 pA/mM.mm².

Original language	English
Pages (from-to)	S336-S339
Journal	Journal of the Korean Physical Society
Volume	42
Issue number	SPEC.
Publication status	Published - 2003 Feb
Event	Proceedings of The 11th Seoul International Symposium on the Physics of Semiconductors and Apllications - 2002 - Cheju Island, Korea, Republic of Duration: 2002 Aug 20 → 2002 Aug 23

Keywords

Biosensor
Chemiluminescence
Luminol
Photodiode

ASJC Scopus subject areas

General Physics and Astronomy

Cite this

@article{b23cfa36c4ce454b9f9ebcba95717d92,

title = "Fabrication and characterization of silicon-based photodiode for detection of luminol chemiluminescence in a biosensor",

abstract = "In this paper, a silicon-based p+-n junction photodiode is fabricated by semiconductor processing as a detector for luminol chemiluminescence (CL) that is used in present the biosensor. The optimum conditions of the CL reaction are determined by horse-radish peroxidase (HRP) concentration, the reagent mixture ratio of luminol with HRP and hydrogen peroxide (H2O2) in the reactor well made of polydimethylsiloxane (PDMS). The output current of the photodiode is measured for CL by H2O2 concentration under 2 mM luminol and 25 units HRP. As the H2O2 concentration increases, the photodiode output current peak values increase. The photocurrent is linearly related to the H2O2 concentration in the range 0.01 ∼ 0.5 mM, and then the sensitivity of the photodiode is 23.429 pA/mM.mm2.",

keywords = "Biosensor, Chemiluminescence, Luminol, Photodiode",

author = "Kim, {Hyung Il} and Kang, {Chul Goo} and Kang, {Moon Sik} and Park, {Jung Ho} and Min, {Nam Ki}",

year = "2003",

month = feb,

language = "English",

volume = "42",

pages = "S336--S339",

journal = "Journal of the Korean Physical Society",

issn = "0374-4884",

publisher = "Korean Physical Society",

number = "SPEC.",

note = "Proceedings of The 11th Seoul International Symposium on the Physics of Semiconductors and Apllications - 2002 ; Conference date: 20-08-2002 Through 23-08-2002",

}

TY - JOUR

T1 - Fabrication and characterization of silicon-based photodiode for detection of luminol chemiluminescence in a biosensor

AU - Kim, Hyung Il

AU - Kang, Chul Goo

AU - Kang, Moon Sik

AU - Park, Jung Ho

AU - Min, Nam Ki

PY - 2003/2

Y1 - 2003/2

N2 - In this paper, a silicon-based p+-n junction photodiode is fabricated by semiconductor processing as a detector for luminol chemiluminescence (CL) that is used in present the biosensor. The optimum conditions of the CL reaction are determined by horse-radish peroxidase (HRP) concentration, the reagent mixture ratio of luminol with HRP and hydrogen peroxide (H2O2) in the reactor well made of polydimethylsiloxane (PDMS). The output current of the photodiode is measured for CL by H2O2 concentration under 2 mM luminol and 25 units HRP. As the H2O2 concentration increases, the photodiode output current peak values increase. The photocurrent is linearly related to the H2O2 concentration in the range 0.01 ∼ 0.5 mM, and then the sensitivity of the photodiode is 23.429 pA/mM.mm2.

AB - In this paper, a silicon-based p+-n junction photodiode is fabricated by semiconductor processing as a detector for luminol chemiluminescence (CL) that is used in present the biosensor. The optimum conditions of the CL reaction are determined by horse-radish peroxidase (HRP) concentration, the reagent mixture ratio of luminol with HRP and hydrogen peroxide (H2O2) in the reactor well made of polydimethylsiloxane (PDMS). The output current of the photodiode is measured for CL by H2O2 concentration under 2 mM luminol and 25 units HRP. As the H2O2 concentration increases, the photodiode output current peak values increase. The photocurrent is linearly related to the H2O2 concentration in the range 0.01 ∼ 0.5 mM, and then the sensitivity of the photodiode is 23.429 pA/mM.mm2.

KW - Biosensor

KW - Chemiluminescence

KW - Luminol

KW - Photodiode

UR - http://www.scopus.com/inward/record.url?scp=0037307161&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037307161&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:0037307161

SN - 0374-4884

VL - 42

SP - S336-S339

JO - Journal of the Korean Physical Society

JF - Journal of the Korean Physical Society

IS - SPEC.

T2 - Proceedings of The 11th Seoul International Symposium on the Physics of Semiconductors and Apllications - 2002

Y2 - 20 August 2002 through 23 August 2002

ER -

Fabrication and characterization of silicon-based photodiode for detection of luminol chemiluminescence in a biosensor

Abstract

Keywords

ASJC Scopus subject areas

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