Fabrication and testing of a PDMS multi-stacked hand-operated LOC for use in portable immunosensing systems

Sin Wook Park; Jun Hwang Lee; Hyun C. Yoon; Byung Woo Kim; Sang Jun Sim; Heeyeop Chae; Sang Sik Yang

doi:10.1007/s10544-008-9200-9

Fabrication and testing of a PDMS multi-stacked hand-operated LOC for use in portable immunosensing systems

Sin Wook Park, Jun Hwang Lee, Hyun C. Yoon, Byung Woo Kim, Sang Jun Sim, Heeyeop Chae, Sang Sik Yang

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

This paper presents the development of a reliable multi-liquid lab-on-a-chip (LOC), with a hand-operated mechanism, for the application in portable immunosensing systems. To control the transport of multiple liquids without any external equipment, we utilize capillary attraction force for filling and surface tension for stopping liquid flow. As a driving force, hydraulic pressure caused by the elastic deformation of a liquid reservoir transfers liquid stopped at passive valves. The proposed LOC successfully demonstrates a reliable sequential liquid transfer within the reaction channel. To highlight its feasibility as a portable diagnostic system, we performed the electrochemical immunoassay measuring antibody concentrations within the fabricated LOC. As a test biorecognition reaction, the anti-dinitrophenyl (DNP) antibody with an enzymatic catalysis was selected as the target analyte. The amplified signals obtained from this experiment indicated a high selectivity of the immunosensing LOC.

Original language	English
Pages (from-to)	859-868
Number of pages	10
Journal	Biomedical Microdevices
Volume	10
Issue number	6
DOIs	https://doi.org/10.1007/s10544-008-9200-9
Publication status	Published - 2008
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgements This work was supported by a grant (No. RTI04-03-05) from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy (MOCIE).

Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.

Keywords

Hand-operating
Immunosensing
Lab-on-a-chip
Microfluidics
Surface tension

ASJC Scopus subject areas

Biomedical Engineering
Molecular Biology

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10.1007/s10544-008-9200-9

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title = "Fabrication and testing of a PDMS multi-stacked hand-operated LOC for use in portable immunosensing systems",

abstract = "This paper presents the development of a reliable multi-liquid lab-on-a-chip (LOC), with a hand-operated mechanism, for the application in portable immunosensing systems. To control the transport of multiple liquids without any external equipment, we utilize capillary attraction force for filling and surface tension for stopping liquid flow. As a driving force, hydraulic pressure caused by the elastic deformation of a liquid reservoir transfers liquid stopped at passive valves. The proposed LOC successfully demonstrates a reliable sequential liquid transfer within the reaction channel. To highlight its feasibility as a portable diagnostic system, we performed the electrochemical immunoassay measuring antibody concentrations within the fabricated LOC. As a test biorecognition reaction, the anti-dinitrophenyl (DNP) antibody with an enzymatic catalysis was selected as the target analyte. The amplified signals obtained from this experiment indicated a high selectivity of the immunosensing LOC.",

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author = "Park, {Sin Wook} and Lee, {Jun Hwang} and Yoon, {Hyun C.} and Kim, {Byung Woo} and Sim, {Sang Jun} and Heeyeop Chae and Yang, {Sang Sik}",

note = "Funding Information: Acknowledgements This work was supported by a grant (No. RTI04-03-05) from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy (MOCIE). Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

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doi = "10.1007/s10544-008-9200-9",

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AU - Park, Sin Wook

AU - Lee, Jun Hwang

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AU - Kim, Byung Woo

AU - Sim, Sang Jun

AU - Chae, Heeyeop

AU - Yang, Sang Sik

N1 - Funding Information: Acknowledgements This work was supported by a grant (No. RTI04-03-05) from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy (MOCIE). Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2008

Y1 - 2008

N2 - This paper presents the development of a reliable multi-liquid lab-on-a-chip (LOC), with a hand-operated mechanism, for the application in portable immunosensing systems. To control the transport of multiple liquids without any external equipment, we utilize capillary attraction force for filling and surface tension for stopping liquid flow. As a driving force, hydraulic pressure caused by the elastic deformation of a liquid reservoir transfers liquid stopped at passive valves. The proposed LOC successfully demonstrates a reliable sequential liquid transfer within the reaction channel. To highlight its feasibility as a portable diagnostic system, we performed the electrochemical immunoassay measuring antibody concentrations within the fabricated LOC. As a test biorecognition reaction, the anti-dinitrophenyl (DNP) antibody with an enzymatic catalysis was selected as the target analyte. The amplified signals obtained from this experiment indicated a high selectivity of the immunosensing LOC.

AB - This paper presents the development of a reliable multi-liquid lab-on-a-chip (LOC), with a hand-operated mechanism, for the application in portable immunosensing systems. To control the transport of multiple liquids without any external equipment, we utilize capillary attraction force for filling and surface tension for stopping liquid flow. As a driving force, hydraulic pressure caused by the elastic deformation of a liquid reservoir transfers liquid stopped at passive valves. The proposed LOC successfully demonstrates a reliable sequential liquid transfer within the reaction channel. To highlight its feasibility as a portable diagnostic system, we performed the electrochemical immunoassay measuring antibody concentrations within the fabricated LOC. As a test biorecognition reaction, the anti-dinitrophenyl (DNP) antibody with an enzymatic catalysis was selected as the target analyte. The amplified signals obtained from this experiment indicated a high selectivity of the immunosensing LOC.

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KW - Microfluidics

KW - Surface tension

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Fabrication and testing of a PDMS multi-stacked hand-operated LOC for use in portable immunosensing systems

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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