Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering

Sung Eun Kim; Young Pil Yun; Kyu Sik Shim; Kyeongsoon Park; Sung Wook Choi; Dong Hyup Shin; Dong Hun Suh

doi:10.1016/j.colsurfb.2015.05.003

Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering

Sung Eun Kim, Young Pil Yun, Kyu Sik Shim, Kyeongsoon Park, Sung Wook Choi, Dong Hyup Shin, Dong Hun Suh

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

The purpose of this study was to fabricate BMP-2-immobilized porous poly(lactide-co-glycolide) (PLGA) microspheres (PMS) modified with heparin for bone regeneration. A fluidic device was used to fabricate PMS and the fabricated PMS was modified with heparin-dopamine (Hep-DOPA). Bone morphogenic protein-2 (BMP-2) was immobilized on the heparinized PMS (Hep-PMS) via electrostatic interactions. Both PMS and modified PMS were characterized using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). MG-63 cell activity on PMS and modified PMS were assessed via alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Immobilized Hep-DOPA and BMP-2 on PMS were demonstrated by XPS analysis. BMP-2-immobilized Hep-PMS provided significantly higher ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression compared to PMS alone. These results suggest that BMP-2-immobilized Hep-PMS effectively improves MG-63 cell activity. In conclusion, BMP-2-immobilized Hep-PMS can be used to effectively regenerate bone defects.

Original language	English
Pages (from-to)	453-460
Number of pages	8
Journal	Colloids and Surfaces B: Biointerfaces
Volume	134
DOIs	https://doi.org/10.1016/j.colsurfb.2015.05.003
Publication status	Published - 2015 Oct 1

Keywords

Bone morphogenic protein-2 (BMP-2)
Bone tissue engineering
Fluidic device
Heparin
Porous microspheres

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/j.colsurfb.2015.05.003

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title = "Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering",

abstract = "The purpose of this study was to fabricate BMP-2-immobilized porous poly(lactide-co-glycolide) (PLGA) microspheres (PMS) modified with heparin for bone regeneration. A fluidic device was used to fabricate PMS and the fabricated PMS was modified with heparin-dopamine (Hep-DOPA). Bone morphogenic protein-2 (BMP-2) was immobilized on the heparinized PMS (Hep-PMS) via electrostatic interactions. Both PMS and modified PMS were characterized using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). MG-63 cell activity on PMS and modified PMS were assessed via alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Immobilized Hep-DOPA and BMP-2 on PMS were demonstrated by XPS analysis. BMP-2-immobilized Hep-PMS provided significantly higher ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression compared to PMS alone. These results suggest that BMP-2-immobilized Hep-PMS effectively improves MG-63 cell activity. In conclusion, BMP-2-immobilized Hep-PMS can be used to effectively regenerate bone defects.",

keywords = "Bone morphogenic protein-2 (BMP-2), Bone tissue engineering, Fluidic device, Heparin, Porous microspheres",

author = "Kim, {Sung Eun} and Yun, {Young Pil} and Shim, {Kyu Sik} and Kyeongsoon Park and Choi, {Sung Wook} and Shin, {Dong Hyup} and Suh, {Dong Hun}",

note = "Funding Information: This study was supported by a Korea University Grant K1300081 . Publisher Copyright: {\textcopyright} 2015 Elsevier B.V.",

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AU - Kim, Sung Eun

AU - Yun, Young Pil

AU - Shim, Kyu Sik

AU - Park, Kyeongsoon

AU - Choi, Sung Wook

AU - Shin, Dong Hyup

AU - Suh, Dong Hun

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N2 - The purpose of this study was to fabricate BMP-2-immobilized porous poly(lactide-co-glycolide) (PLGA) microspheres (PMS) modified with heparin for bone regeneration. A fluidic device was used to fabricate PMS and the fabricated PMS was modified with heparin-dopamine (Hep-DOPA). Bone morphogenic protein-2 (BMP-2) was immobilized on the heparinized PMS (Hep-PMS) via electrostatic interactions. Both PMS and modified PMS were characterized using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). MG-63 cell activity on PMS and modified PMS were assessed via alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Immobilized Hep-DOPA and BMP-2 on PMS were demonstrated by XPS analysis. BMP-2-immobilized Hep-PMS provided significantly higher ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression compared to PMS alone. These results suggest that BMP-2-immobilized Hep-PMS effectively improves MG-63 cell activity. In conclusion, BMP-2-immobilized Hep-PMS can be used to effectively regenerate bone defects.

AB - The purpose of this study was to fabricate BMP-2-immobilized porous poly(lactide-co-glycolide) (PLGA) microspheres (PMS) modified with heparin for bone regeneration. A fluidic device was used to fabricate PMS and the fabricated PMS was modified with heparin-dopamine (Hep-DOPA). Bone morphogenic protein-2 (BMP-2) was immobilized on the heparinized PMS (Hep-PMS) via electrostatic interactions. Both PMS and modified PMS were characterized using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). MG-63 cell activity on PMS and modified PMS were assessed via alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Immobilized Hep-DOPA and BMP-2 on PMS were demonstrated by XPS analysis. BMP-2-immobilized Hep-PMS provided significantly higher ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression compared to PMS alone. These results suggest that BMP-2-immobilized Hep-PMS effectively improves MG-63 cell activity. In conclusion, BMP-2-immobilized Hep-PMS can be used to effectively regenerate bone defects.

KW - Bone morphogenic protein-2 (BMP-2)

KW - Bone tissue engineering

KW - Fluidic device

KW - Heparin

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Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering

Abstract

Keywords

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