Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells

Yong Kwan Noh, Avelino Dos Santos Da Costa, Yong Seek Park, Ping Du, Ik Hwan Kim, Kwideok Park

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)


Scaffold plays a critical role in stem cell differentiation and tissue regeneration. Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1) were fabricated in this study. The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%. They were also favorable with cell attachment and growth. After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold. When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1). The current work demonstrates that our BC/Col composites may be promising as a bone tissue-engineered scaffold.

Original languageEnglish
Pages (from-to)210-218
Number of pages9
JournalCarbohydrate Polymers
Publication statusPublished - 2019 Sept 1

Bibliographical note

Funding Information:
This work was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) , funded by the Ministry of Health and Welfare ( HI17C1234 ), Republic of Korea.

Publisher Copyright:
© 2019 Elsevier Ltd


  • Bacterial cellulose (BC)
  • Bone regeneration
  • Collagen
  • Osteogenesis
  • Umbilical cord blood derived mesenchymal stem cells (UCB-MSCs)

ASJC Scopus subject areas

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry


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