TY - JOUR
T1 - Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells
AU - Noh, Yong Kwan
AU - Dos Santos Da Costa, Avelino
AU - Park, Yong Seek
AU - Du, Ping
AU - Kim, Ik Hwan
AU - Park, Kwideok
N1 - Funding Information:
This work was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) , funded by the Ministry of Health and Welfare ( HI17C1234 ), Republic of Korea.
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Scaffold plays a critical role in stem cell differentiation and tissue regeneration. Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1) were fabricated in this study. The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%. They were also favorable with cell attachment and growth. After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold. When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1). The current work demonstrates that our BC/Col composites may be promising as a bone tissue-engineered scaffold.
AB - Scaffold plays a critical role in stem cell differentiation and tissue regeneration. Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1) were fabricated in this study. The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%. They were also favorable with cell attachment and growth. After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold. When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1). The current work demonstrates that our BC/Col composites may be promising as a bone tissue-engineered scaffold.
KW - Bacterial cellulose (BC)
KW - Bone regeneration
KW - Collagen
KW - Osteogenesis
KW - Umbilical cord blood derived mesenchymal stem cells (UCB-MSCs)
UR - http://www.scopus.com/inward/record.url?scp=85065639152&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2019.05.039
DO - 10.1016/j.carbpol.2019.05.039
M3 - Article
C2 - 31151519
AN - SCOPUS:85065639152
SN - 0144-8617
VL - 219
SP - 210
EP - 218
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -