Fabrication of type i collagen microcarrier using a microfluidic 3D T-junction device and its application for the quantitative analysis of cell-ECM interactions

Junghyo Yoon; Jaehoon Kim; Hyo Eun Jeong; Ryo Sudo; Myung Jin Park; Seok Chung

doi:10.1088/1758-5090/8/3/035014

Fabrication of type i collagen microcarrier using a microfluidic 3D T-junction device and its application for the quantitative analysis of cell-ECM interactions

Junghyo Yoon, Jaehoon Kim, Hyo Eun Jeong, Ryo Sudo, Myung Jin Park, Seok Chung

School of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We presented a new quantitative analysis for cell and extracellular matrix (ECM) interactions, using cell-coated ECM hydrogel microbeads (hydrobeads) made of type I collagen. The hydrobeads can carry cells as three-dimensional spheroidal forms with an ECM inside, facilitating a direct interaction between the cells and ECM. The cells on hydrobeads do not have a hypoxic core, which opens the possibility for using as a cell microcarrier for bottom-up tissue reconstitution. This technique can utilize various types of cells, even MDA-MB-231 cells, which have weak cell-cell interactions and do not form spheroids in conventional spheroid culture methods. Morphological indices of the cell-coated hydrobead visually present cell-ECM interactions in a quantitative manner.

Original language	English
Article number	035014
Journal	Biofabrication
Volume	8
Issue number	3
DOIs	https://doi.org/10.1088/1758-5090/8/3/035014
Publication status	Published - 2016 Aug 26

Bibliographical note

Funding Information:
This research was supported by the National R and D Program through the Korea Institute of Radiological and Medical Sciences funded by the Ministry of Science, ICT & Future Planning (No.1711021925) and by National Research Foundation of Korea (2014M3A7B4052193).

Publisher Copyright:
© 2016 IOP Publishing Ltd.

Keywords

collagen
extracellular matrix
hydrobead
remodeling

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Biomaterials
Biomedical Engineering

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10.1088/1758-5090/8/3/035014

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abstract = "We presented a new quantitative analysis for cell and extracellular matrix (ECM) interactions, using cell-coated ECM hydrogel microbeads (hydrobeads) made of type I collagen. The hydrobeads can carry cells as three-dimensional spheroidal forms with an ECM inside, facilitating a direct interaction between the cells and ECM. The cells on hydrobeads do not have a hypoxic core, which opens the possibility for using as a cell microcarrier for bottom-up tissue reconstitution. This technique can utilize various types of cells, even MDA-MB-231 cells, which have weak cell-cell interactions and do not form spheroids in conventional spheroid culture methods. Morphological indices of the cell-coated hydrobead visually present cell-ECM interactions in a quantitative manner.",

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AU - Kim, Jaehoon

AU - Jeong, Hyo Eun

AU - Sudo, Ryo

AU - Park, Myung Jin

AU - Chung, Seok

N1 - Funding Information: This research was supported by the National R and D Program through the Korea Institute of Radiological and Medical Sciences funded by the Ministry of Science, ICT & Future Planning (No.1711021925) and by National Research Foundation of Korea (2014M3A7B4052193). Publisher Copyright: © 2016 IOP Publishing Ltd.

PY - 2016/8/26

Y1 - 2016/8/26

N2 - We presented a new quantitative analysis for cell and extracellular matrix (ECM) interactions, using cell-coated ECM hydrogel microbeads (hydrobeads) made of type I collagen. The hydrobeads can carry cells as three-dimensional spheroidal forms with an ECM inside, facilitating a direct interaction between the cells and ECM. The cells on hydrobeads do not have a hypoxic core, which opens the possibility for using as a cell microcarrier for bottom-up tissue reconstitution. This technique can utilize various types of cells, even MDA-MB-231 cells, which have weak cell-cell interactions and do not form spheroids in conventional spheroid culture methods. Morphological indices of the cell-coated hydrobead visually present cell-ECM interactions in a quantitative manner.

AB - We presented a new quantitative analysis for cell and extracellular matrix (ECM) interactions, using cell-coated ECM hydrogel microbeads (hydrobeads) made of type I collagen. The hydrobeads can carry cells as three-dimensional spheroidal forms with an ECM inside, facilitating a direct interaction between the cells and ECM. The cells on hydrobeads do not have a hypoxic core, which opens the possibility for using as a cell microcarrier for bottom-up tissue reconstitution. This technique can utilize various types of cells, even MDA-MB-231 cells, which have weak cell-cell interactions and do not form spheroids in conventional spheroid culture methods. Morphological indices of the cell-coated hydrobead visually present cell-ECM interactions in a quantitative manner.

KW - collagen

KW - extracellular matrix

KW - hydrobead

KW - remodeling

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Fabrication of type i collagen microcarrier using a microfluidic 3D T-junction device and its application for the quantitative analysis of cell-ECM interactions

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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