Failure of biohydrogen production by low levels of substrate and lactic acid accumulation

Jeong Hoon Park; Sang Hoon Lee; Hyun Jun Ju; Sang Hyoun Kim; Jeong Jun Yoon; Hee Deung Park

doi:10.1016/j.renene.2015.09.016

Failure of biohydrogen production by low levels of substrate and lactic acid accumulation

Jeong Hoon Park, Sang Hoon Lee, Hyun Jun Ju, Sang Hyoun Kim, Jeong Jun Yoon, Hee Deung Park

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

To identify causes affecting the stability of biohydrogen production from the microbial community perspective, a fermentor was operated using galactose as a substrate for 30 days. The fermentor demonstrated that hydrogen production yield gradually increased with concomitant decrease of galactose concentration until day 27. Meanwhile, hydrogen producing clostridia gradually increased their proportion to 75% of bacteria analyzed until day 11, and then gradually decreased to 23% with increases in other groups of bacteria such as Bacilli. After day 27, hydrogen production abruptly failed without deterioration of galactose utilization. At that time, lactic acid accumulated to 920 mg COD/L and Ruminococcaceae increased its proportion to 30%. In conclusion, low substrate levels, beginning on day 11, provided a selective disadvantage to the hydrogen producing clostridia and proliferated other groups of bacteria, which resulted in accumulation of lactic acid and, ultimately, failure of hydrogen production.

Original language	English
Pages (from-to)	889-894
Number of pages	6
Journal	Renewable Energy
Volume	86
DOIs	https://doi.org/10.1016/j.renene.2015.09.016
Publication status	Published - 2016 Feb 1

Bibliographical note

Funding Information:
We would like to thank Samantha Reuter for proofreading this manuscript and the anonymous reviewers for their careful comments on the manuscript. This research was funded by the Korea Ministry of Environment (Global Top Project: GT-13-B-02-001-1 ).

Publisher Copyright:
© 2015 Elsevier Ltd.

Keywords

Biohydrogen
Clostridium
Galactose
Lactic acid
Substrate competition

ASJC Scopus subject areas

Renewable Energy, Sustainability and the Environment

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.renene.2015.09.016

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title = "Failure of biohydrogen production by low levels of substrate and lactic acid accumulation",

abstract = "To identify causes affecting the stability of biohydrogen production from the microbial community perspective, a fermentor was operated using galactose as a substrate for 30 days. The fermentor demonstrated that hydrogen production yield gradually increased with concomitant decrease of galactose concentration until day 27. Meanwhile, hydrogen producing clostridia gradually increased their proportion to 75% of bacteria analyzed until day 11, and then gradually decreased to 23% with increases in other groups of bacteria such as Bacilli. After day 27, hydrogen production abruptly failed without deterioration of galactose utilization. At that time, lactic acid accumulated to 920 mg COD/L and Ruminococcaceae increased its proportion to 30%. In conclusion, low substrate levels, beginning on day 11, provided a selective disadvantage to the hydrogen producing clostridia and proliferated other groups of bacteria, which resulted in accumulation of lactic acid and, ultimately, failure of hydrogen production.",

keywords = "Biohydrogen, Clostridium, Galactose, Lactic acid, Substrate competition",

author = "Park, {Jeong Hoon} and Lee, {Sang Hoon} and Ju, {Hyun Jun} and Kim, {Sang Hyoun} and Yoon, {Jeong Jun} and Park, {Hee Deung}",

note = "Funding Information: We would like to thank Samantha Reuter for proofreading this manuscript and the anonymous reviewers for their careful comments on the manuscript. This research was funded by the Korea Ministry of Environment (Global Top Project: GT-13-B-02-001-1 ). Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd.",

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doi = "10.1016/j.renene.2015.09.016",

language = "English",

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AU - Park, Jeong Hoon

AU - Lee, Sang Hoon

AU - Ju, Hyun Jun

AU - Kim, Sang Hyoun

AU - Yoon, Jeong Jun

AU - Park, Hee Deung

N1 - Funding Information: We would like to thank Samantha Reuter for proofreading this manuscript and the anonymous reviewers for their careful comments on the manuscript. This research was funded by the Korea Ministry of Environment (Global Top Project: GT-13-B-02-001-1 ). Publisher Copyright: © 2015 Elsevier Ltd.

PY - 2016/2/1

Y1 - 2016/2/1

N2 - To identify causes affecting the stability of biohydrogen production from the microbial community perspective, a fermentor was operated using galactose as a substrate for 30 days. The fermentor demonstrated that hydrogen production yield gradually increased with concomitant decrease of galactose concentration until day 27. Meanwhile, hydrogen producing clostridia gradually increased their proportion to 75% of bacteria analyzed until day 11, and then gradually decreased to 23% with increases in other groups of bacteria such as Bacilli. After day 27, hydrogen production abruptly failed without deterioration of galactose utilization. At that time, lactic acid accumulated to 920 mg COD/L and Ruminococcaceae increased its proportion to 30%. In conclusion, low substrate levels, beginning on day 11, provided a selective disadvantage to the hydrogen producing clostridia and proliferated other groups of bacteria, which resulted in accumulation of lactic acid and, ultimately, failure of hydrogen production.

AB - To identify causes affecting the stability of biohydrogen production from the microbial community perspective, a fermentor was operated using galactose as a substrate for 30 days. The fermentor demonstrated that hydrogen production yield gradually increased with concomitant decrease of galactose concentration until day 27. Meanwhile, hydrogen producing clostridia gradually increased their proportion to 75% of bacteria analyzed until day 11, and then gradually decreased to 23% with increases in other groups of bacteria such as Bacilli. After day 27, hydrogen production abruptly failed without deterioration of galactose utilization. At that time, lactic acid accumulated to 920 mg COD/L and Ruminococcaceae increased its proportion to 30%. In conclusion, low substrate levels, beginning on day 11, provided a selective disadvantage to the hydrogen producing clostridia and proliferated other groups of bacteria, which resulted in accumulation of lactic acid and, ultimately, failure of hydrogen production.

KW - Biohydrogen

KW - Clostridium

KW - Galactose

KW - Lactic acid

KW - Substrate competition

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DO - 10.1016/j.renene.2015.09.016

M3 - Article

AN - SCOPUS:84941985614

SN - 0960-1481

VL - 86

SP - 889

EP - 894

JO - Renewable Energy

JF - Renewable Energy

ER -

Failure of biohydrogen production by low levels of substrate and lactic acid accumulation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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