Abstract
In order to obtain high productivity of clavulanic acid, a newly-introduced carrier, polyurethane pellet (PUP) Z97-020 was used for the immobilization process. In a stirred-tank bioreactor, batch cultivation by Streptomyces clavuligerus KK immobilized on PUP Z97-020 gave about 3100 mg of clavulanic acid per litre, representing an increase of 200% in productivity compared with that by fed-batch cultivation of free cells (1500 mg/l). However, the clavulanic acid produced rapidly decomposed due to the pH change during batch cultivation. Fed-batch cultivation by immobilized S. clavuligerus KK gave an excellent level of clavulanic acid up to 3250 mg/l, a productivity increase of 220% compared with that by fed-batch cultivation of free cells. These results suggest that immobilization with PUP Z97-020 is a more effective process for the production of clavulanic acid and that the maintenance of pH by fed-batch cultivation with glycerol as a limiting substrate prevents the clavulanic acid from decomposing during the fermentation.
| Original language | English |
|---|---|
| Pages (from-to) | 869-872 |
| Number of pages | 4 |
| Journal | World Journal of Microbiology and Biotechnology |
| Volume | 17 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2001 |
Bibliographical note
Funding Information:This study was supported by research grants from the Korea Science and Engineering Foundation (KOSEF) through the Applied Rheology Center (ARC), an official KOSEF-created engineering research center (ERC) at Korea University, Seoul, Korea.
Keywords
- Clavulanic acid
- Fed-batch cultivation
- Immobilization
- Streptomyces clavuligerus KK
ASJC Scopus subject areas
- Biotechnology
- Physiology
- Applied Microbiology and Biotechnology