A fed-batch culture process followed by subsequent photoautotrophic induction was established for the high density culture of astaxanthin-rich Haematococcus pluvialis using a CO2-fed flat type photobioreactor under unsynchronized illumination. Fed-batch culture was performed with an exponential feeding strategy of the growth-limiting nutrients, nitrate and phosphate, concurrently with the stepwise supplementation of light depending on the cell concentration. During the growth phase, a biomass of 1.47 g/L was obtained at a biomass productivity of 0.33 g/L/day. Photoautotrophic induction of the well-grown vegetative cells was performed consecutively by increasing the light intensity to 400 μmol photon/m2/s, while keeping the other conditions in the CO2-fed flat type photobioreactor fixed, yielding an astaxanthin production of 190 mg/L at an astaxanthin productivity of 14 mg/L/day. The proposed sequential photoautotrophic process has high potential as simple and productive process for the production of valuable Haematococcus astaxanthin.
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Acknowledgment This research was supported by a grant (DG2-201) from Carbon Dioxide Reduction & Sequestration Research Center, one of the Twenty-first Century Frontier Programs funded by the Ministry of Science and Technology of Korean government.
Copyright 2010 Elsevier B.V., All rights reserved.
- Exponential feeding
- Fed-batch culture
- Haematococcus pluvialis
- Photoautotrophic induction
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