Fed-batch culture of astaxanthin-rich Haematococcus pluvialis by exponential nutrient feeding and stepwise light supplementation

Chang Duk Kang, Se Jong Han, Seung Phill Choi, Sang Jun Sim

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46 Citations (Scopus)


A fed-batch culture process followed by subsequent photoautotrophic induction was established for the high density culture of astaxanthin-rich Haematococcus pluvialis using a CO2-fed flat type photobioreactor under unsynchronized illumination. Fed-batch culture was performed with an exponential feeding strategy of the growth-limiting nutrients, nitrate and phosphate, concurrently with the stepwise supplementation of light depending on the cell concentration. During the growth phase, a biomass of 1.47 g/L was obtained at a biomass productivity of 0.33 g/L/day. Photoautotrophic induction of the well-grown vegetative cells was performed consecutively by increasing the light intensity to 400 μmol photon/m2/s, while keeping the other conditions in the CO2-fed flat type photobioreactor fixed, yielding an astaxanthin production of 190 mg/L at an astaxanthin productivity of 14 mg/L/day. The proposed sequential photoautotrophic process has high potential as simple and productive process for the production of valuable Haematococcus astaxanthin.

Original languageEnglish
Pages (from-to)133-139
Number of pages7
JournalBioprocess and Biosystems Engineering
Issue number1
Publication statusPublished - 2010
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgment This research was supported by a grant (DG2-201) from Carbon Dioxide Reduction & Sequestration Research Center, one of the Twenty-first Century Frontier Programs funded by the Ministry of Science and Technology of Korean government.

Copyright 2010 Elsevier B.V., All rights reserved.


  • Astaxanthin
  • Exponential feeding
  • Fed-batch culture
  • Haematococcus pluvialis
  • Photoautotrophic induction

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering


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