Abstract
A fed-batch culture process followed by subsequent photoautotrophic induction was established for the high density culture of astaxanthin-rich Haematococcus pluvialis using a CO2-fed flat type photobioreactor under unsynchronized illumination. Fed-batch culture was performed with an exponential feeding strategy of the growth-limiting nutrients, nitrate and phosphate, concurrently with the stepwise supplementation of light depending on the cell concentration. During the growth phase, a biomass of 1.47 g/L was obtained at a biomass productivity of 0.33 g/L/day. Photoautotrophic induction of the well-grown vegetative cells was performed consecutively by increasing the light intensity to 400 μmol photon/m2/s, while keeping the other conditions in the CO2-fed flat type photobioreactor fixed, yielding an astaxanthin production of 190 mg/L at an astaxanthin productivity of 14 mg/L/day. The proposed sequential photoautotrophic process has high potential as simple and productive process for the production of valuable Haematococcus astaxanthin.
Original language | English |
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Pages (from-to) | 133-139 |
Number of pages | 7 |
Journal | Bioprocess and Biosystems Engineering |
Volume | 33 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2010 |
Externally published | Yes |
Keywords
- Astaxanthin
- Exponential feeding
- Fed-batch culture
- Haematococcus pluvialis
- Photoautotrophic induction
ASJC Scopus subject areas
- Biotechnology
- Bioengineering