Feed-back regulation of disabled-2 (Dab2) p96 isoform for GATA-4 during differentiation of F9 cells

Jung Ah Kim; Seong Ho Bae; Young Joon Choi; Kyung Hyun Kim; Sung Soo Park

doi:10.1016/j.bbrc.2012.04.051

Feed-back regulation of disabled-2 (Dab2) p96 isoform for GATA-4 during differentiation of F9 cells

Jung Ah Kim, Seong Ho Bae, Young Joon Choi, Kyung Hyun Kim, Sung Soo Park

* Honorary professors

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

F9 embryonic carcinoma (EC) cells undergo extra-embryonic endodermal (ExE) differentiation in response to retinoic acid (RA) treatment, which induces the expression of two isoforms (p96 and p67) of the adaptor protein, Disabled-2 (Dab2). In the current study, constitutive and ectopic expression of the p96 isoform induced ExE differentiation in F9 EC cells in the absence of RA treatment via the activation of GATA-4 by p96. During the RA-induced differentiation process, Dab2 expression is induced by the GATA factors in a coherent feed-forward loop; on the other hand, we showed that p96 regulates GATA-4 in a positive feed-back manner in this study. Our results indicate that p96 Dab2 plays a key role in the ExE differentiation process.

Original language	English
Pages (from-to)	591-598
Number of pages	8
Journal	Biochemical and biophysical research communications
Volume	421
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2012.04.051
Publication status	Published - 2012 May 11

Keywords

Disabled-2 (Dab2)
Extra-embryonic endoderm (ExE)
Feed-back loop
GATA-4

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2012.04.051

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@article{9ef00f254954450689df2db574b6ffd5,

title = "Feed-back regulation of disabled-2 (Dab2) p96 isoform for GATA-4 during differentiation of F9 cells",

abstract = "F9 embryonic carcinoma (EC) cells undergo extra-embryonic endodermal (ExE) differentiation in response to retinoic acid (RA) treatment, which induces the expression of two isoforms (p96 and p67) of the adaptor protein, Disabled-2 (Dab2). In the current study, constitutive and ectopic expression of the p96 isoform induced ExE differentiation in F9 EC cells in the absence of RA treatment via the activation of GATA-4 by p96. During the RA-induced differentiation process, Dab2 expression is induced by the GATA factors in a coherent feed-forward loop; on the other hand, we showed that p96 regulates GATA-4 in a positive feed-back manner in this study. Our results indicate that p96 Dab2 plays a key role in the ExE differentiation process.",

keywords = "Disabled-2 (Dab2), Extra-embryonic endoderm (ExE), Feed-back loop, GATA-4",

author = "Kim, {Jung Ah} and Bae, {Seong Ho} and Choi, {Young Joon} and Kim, {Kyung Hyun} and Park, {Sung Soo}",

note = "Funding Information: This study was mostly aided by Grant No. ( R01-2006-000-11256-0 ) from the Basic Research Program of the Korea Science & Engineering Foundation (KOSEF) and was supported in part by professor research fund (2011) provided from Korea University. ",

year = "2012",

month = may,

day = "11",

doi = "10.1016/j.bbrc.2012.04.051",

language = "English",

volume = "421",

pages = "591--598",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Feed-back regulation of disabled-2 (Dab2) p96 isoform for GATA-4 during differentiation of F9 cells

AU - Kim, Jung Ah

AU - Bae, Seong Ho

AU - Choi, Young Joon

AU - Kim, Kyung Hyun

AU - Park, Sung Soo

N1 - Funding Information: This study was mostly aided by Grant No. ( R01-2006-000-11256-0 ) from the Basic Research Program of the Korea Science & Engineering Foundation (KOSEF) and was supported in part by professor research fund (2011) provided from Korea University.

PY - 2012/5/11

Y1 - 2012/5/11

N2 - F9 embryonic carcinoma (EC) cells undergo extra-embryonic endodermal (ExE) differentiation in response to retinoic acid (RA) treatment, which induces the expression of two isoforms (p96 and p67) of the adaptor protein, Disabled-2 (Dab2). In the current study, constitutive and ectopic expression of the p96 isoform induced ExE differentiation in F9 EC cells in the absence of RA treatment via the activation of GATA-4 by p96. During the RA-induced differentiation process, Dab2 expression is induced by the GATA factors in a coherent feed-forward loop; on the other hand, we showed that p96 regulates GATA-4 in a positive feed-back manner in this study. Our results indicate that p96 Dab2 plays a key role in the ExE differentiation process.

AB - F9 embryonic carcinoma (EC) cells undergo extra-embryonic endodermal (ExE) differentiation in response to retinoic acid (RA) treatment, which induces the expression of two isoforms (p96 and p67) of the adaptor protein, Disabled-2 (Dab2). In the current study, constitutive and ectopic expression of the p96 isoform induced ExE differentiation in F9 EC cells in the absence of RA treatment via the activation of GATA-4 by p96. During the RA-induced differentiation process, Dab2 expression is induced by the GATA factors in a coherent feed-forward loop; on the other hand, we showed that p96 regulates GATA-4 in a positive feed-back manner in this study. Our results indicate that p96 Dab2 plays a key role in the ExE differentiation process.

KW - Disabled-2 (Dab2)

KW - Extra-embryonic endoderm (ExE)

KW - Feed-back loop

KW - GATA-4

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SN - 0006-291X

VL - 421

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EP - 598

JO - Biochemical and Biophysical Research Communications

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ER -

Feed-back regulation of disabled-2 (Dab2) p96 isoform for GATA-4 during differentiation of F9 cells

Abstract

Keywords

ASJC Scopus subject areas

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