Feed-back regulation of disabled-2 (Dab2) p96 isoform for GATA-4 during differentiation of F9 cells

Jung Ah Kim, Seong Ho Bae, Young Joon Choi, Kyung Hyun Kim, Sung Soo Park

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    F9 embryonic carcinoma (EC) cells undergo extra-embryonic endodermal (ExE) differentiation in response to retinoic acid (RA) treatment, which induces the expression of two isoforms (p96 and p67) of the adaptor protein, Disabled-2 (Dab2). In the current study, constitutive and ectopic expression of the p96 isoform induced ExE differentiation in F9 EC cells in the absence of RA treatment via the activation of GATA-4 by p96. During the RA-induced differentiation process, Dab2 expression is induced by the GATA factors in a coherent feed-forward loop; on the other hand, we showed that p96 regulates GATA-4 in a positive feed-back manner in this study. Our results indicate that p96 Dab2 plays a key role in the ExE differentiation process.

    Original languageEnglish
    Pages (from-to)591-598
    Number of pages8
    JournalBiochemical and biophysical research communications
    Volume421
    Issue number3
    DOIs
    Publication statusPublished - 2012 May 11

    Bibliographical note

    Funding Information:
    This study was mostly aided by Grant No. ( R01-2006-000-11256-0 ) from the Basic Research Program of the Korea Science & Engineering Foundation (KOSEF) and was supported in part by professor research fund (2011) provided from Korea University.

    Keywords

    • Disabled-2 (Dab2)
    • Extra-embryonic endoderm (ExE)
    • Feed-back loop
    • GATA-4

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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