Abstract
F9 embryonic carcinoma (EC) cells undergo extra-embryonic endodermal (ExE) differentiation in response to retinoic acid (RA) treatment, which induces the expression of two isoforms (p96 and p67) of the adaptor protein, Disabled-2 (Dab2). In the current study, constitutive and ectopic expression of the p96 isoform induced ExE differentiation in F9 EC cells in the absence of RA treatment via the activation of GATA-4 by p96. During the RA-induced differentiation process, Dab2 expression is induced by the GATA factors in a coherent feed-forward loop; on the other hand, we showed that p96 regulates GATA-4 in a positive feed-back manner in this study. Our results indicate that p96 Dab2 plays a key role in the ExE differentiation process.
Original language | English |
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Pages (from-to) | 591-598 |
Number of pages | 8 |
Journal | Biochemical and biophysical research communications |
Volume | 421 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2012 May 11 |
Keywords
- Disabled-2 (Dab2)
- Extra-embryonic endoderm (ExE)
- Feed-back loop
- GATA-4
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology