Femto-molar detection of cancer marker-protein based on immuno-nanoplasmonics at single-nanoparticle scale

Yoochan Hong; Eugene Lee; Minhee Ku; Jin Suck Suh; Dae Sung Yoon; Jaemoon Yang

doi:10.1088/0957-4484/27/18/185103

Femto-molar detection of cancer marker-protein based on immuno-nanoplasmonics at single-nanoparticle scale

Yoochan Hong, Eugene Lee, Minhee Ku, Jin Suck Suh, Dae Sung Yoon, Jaemoon Yang

School of Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We describe an in vitro biomarker sensor based on immuno-silver nanomarbles (iSNMs) and the nanoscattering spectrum imaging analysis system using localized surface plasmon resonance (LSPR). In particular, highly monodisperse SNMs with large figures of merit are prepared, and the sensing substrates are also fabricated using the nanoparticle adsorption method. The high sensitivity of the LSPR sensor based on an SNM is confirmed using various solvents that have different refractive indexes. For the sensitive and specific detection of epithelial cell adhesion molecules (EpCAMs) expressed on cancer cells, the surface of the SNM is conjugated with an anti-EpCAM aptamer, and molecular sensing for the EpCAM expression level is carried out using whole cell lysates from various cancer cell lines. Collectively, we have developed a biomarker-detectable LSPR sensor based on iSNMs, which allows for the sensitive and effective detection of EpCAMs at both the single-cell and femto-molar level.

Original language	English
Article number	185103
Journal	Nanotechnology
Volume	27
Issue number	18
DOIs	https://doi.org/10.1088/0957-4484/27/18/185103
Publication status	Published - 2016 Mar 24

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2012R1A11006248 and 2014R1A1A2059806).

Publisher Copyright:
© 2016 IOP Publishing Ltd.

Keywords

aptamer
cancer
localized surface plasmon resonance
silver nanomarble
single nanoparticle spectroscopy

ASJC Scopus subject areas

Bioengineering
Chemistry(all)
Materials Science(all)
Mechanics of Materials
Mechanical Engineering
Electrical and Electronic Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1088/0957-4484/27/18/185103

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title = "Femto-molar detection of cancer marker-protein based on immuno-nanoplasmonics at single-nanoparticle scale",

abstract = "We describe an in vitro biomarker sensor based on immuno-silver nanomarbles (iSNMs) and the nanoscattering spectrum imaging analysis system using localized surface plasmon resonance (LSPR). In particular, highly monodisperse SNMs with large figures of merit are prepared, and the sensing substrates are also fabricated using the nanoparticle adsorption method. The high sensitivity of the LSPR sensor based on an SNM is confirmed using various solvents that have different refractive indexes. For the sensitive and specific detection of epithelial cell adhesion molecules (EpCAMs) expressed on cancer cells, the surface of the SNM is conjugated with an anti-EpCAM aptamer, and molecular sensing for the EpCAM expression level is carried out using whole cell lysates from various cancer cell lines. Collectively, we have developed a biomarker-detectable LSPR sensor based on iSNMs, which allows for the sensitive and effective detection of EpCAMs at both the single-cell and femto-molar level.",

keywords = "aptamer, cancer, localized surface plasmon resonance, silver nanomarble, single nanoparticle spectroscopy",

author = "Yoochan Hong and Eugene Lee and Minhee Ku and Suh, {Jin Suck} and Yoon, {Dae Sung} and Jaemoon Yang",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2012R1A11006248 and 2014R1A1A2059806). Publisher Copyright: {\textcopyright} 2016 IOP Publishing Ltd.",

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doi = "10.1088/0957-4484/27/18/185103",

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AU - Hong, Yoochan

AU - Lee, Eugene

AU - Ku, Minhee

AU - Suh, Jin Suck

AU - Yoon, Dae Sung

AU - Yang, Jaemoon

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2012R1A11006248 and 2014R1A1A2059806). Publisher Copyright: © 2016 IOP Publishing Ltd.

PY - 2016/3/24

Y1 - 2016/3/24

N2 - We describe an in vitro biomarker sensor based on immuno-silver nanomarbles (iSNMs) and the nanoscattering spectrum imaging analysis system using localized surface plasmon resonance (LSPR). In particular, highly monodisperse SNMs with large figures of merit are prepared, and the sensing substrates are also fabricated using the nanoparticle adsorption method. The high sensitivity of the LSPR sensor based on an SNM is confirmed using various solvents that have different refractive indexes. For the sensitive and specific detection of epithelial cell adhesion molecules (EpCAMs) expressed on cancer cells, the surface of the SNM is conjugated with an anti-EpCAM aptamer, and molecular sensing for the EpCAM expression level is carried out using whole cell lysates from various cancer cell lines. Collectively, we have developed a biomarker-detectable LSPR sensor based on iSNMs, which allows for the sensitive and effective detection of EpCAMs at both the single-cell and femto-molar level.

AB - We describe an in vitro biomarker sensor based on immuno-silver nanomarbles (iSNMs) and the nanoscattering spectrum imaging analysis system using localized surface plasmon resonance (LSPR). In particular, highly monodisperse SNMs with large figures of merit are prepared, and the sensing substrates are also fabricated using the nanoparticle adsorption method. The high sensitivity of the LSPR sensor based on an SNM is confirmed using various solvents that have different refractive indexes. For the sensitive and specific detection of epithelial cell adhesion molecules (EpCAMs) expressed on cancer cells, the surface of the SNM is conjugated with an anti-EpCAM aptamer, and molecular sensing for the EpCAM expression level is carried out using whole cell lysates from various cancer cell lines. Collectively, we have developed a biomarker-detectable LSPR sensor based on iSNMs, which allows for the sensitive and effective detection of EpCAMs at both the single-cell and femto-molar level.

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KW - cancer

KW - localized surface plasmon resonance

KW - silver nanomarble

KW - single nanoparticle spectroscopy

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ER -

Femto-molar detection of cancer marker-protein based on immuno-nanoplasmonics at single-nanoparticle scale

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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