TY - JOUR
T1 - Fetal hematopoietic stem cells express MFG-E8 during mouse embryogenesis
AU - Lee, Jaehun
AU - Choi, Byung Il
AU - Park, Seo Young
AU - An, Su Yeon
AU - Han, Jiyou
AU - Kim, Jong Hoon
N1 - Funding Information:
This work was supported by The Ministry of Science, ICT & Future Planning (MSIP) of the National Research Foundation of Korea (No. 2012M3A9B4028636 and No. 2012M3A9C7050139) and by Korea University Grant.
Publisher Copyright:
© 2015 KSBMB. All rights reserved.
PY - 2015/7
Y1 - 2015/7
N2 - The milk fat globule-EGF-factor 8 protein (MFG-E8) has been identified in various tissues, where it has an important role in intercellular interactions, cellular migration, and neovascularization. Previous studies showed that MFG-E8 is expressed in different cell types under normal and pathophysiological conditions, but its expression in hematopoietic stem cells (HSCs) during hematopoiesis has not been reported. In the present study, we investigated MFG-E8 expression in multiple hematopoietic tissues at different stages of mouse embryogenesis. Using immunohistochemistry, we showed that MFG-E8 was specifically expressed in CD34+ HSCs at all hematopoietic sites, including the yolk sac, aorta-gonad-mesonephros region, placenta and fetal liver, during embryogenesis. Fluorescence-activated cell sorting and polymerase chain reaction analyses demonstrated that CD34+ cells, purified from the fetal liver, expressed additional HSC markers, c-Kit and Sca-1, and that these CD34+ cells, but not CD34− cells, highly expressed MFG-E8. We also found that MFG-E8 was not expressed in HSCs in adult mouse bone marrow, and that its expression was confined to F4/80+ macrophages. Together, this study demonstrates, for the first time, that MFG-8 is expressed in fetal HSC populations, and that MFG-E8 may have a role in embryonic hematopoiesis.
AB - The milk fat globule-EGF-factor 8 protein (MFG-E8) has been identified in various tissues, where it has an important role in intercellular interactions, cellular migration, and neovascularization. Previous studies showed that MFG-E8 is expressed in different cell types under normal and pathophysiological conditions, but its expression in hematopoietic stem cells (HSCs) during hematopoiesis has not been reported. In the present study, we investigated MFG-E8 expression in multiple hematopoietic tissues at different stages of mouse embryogenesis. Using immunohistochemistry, we showed that MFG-E8 was specifically expressed in CD34+ HSCs at all hematopoietic sites, including the yolk sac, aorta-gonad-mesonephros region, placenta and fetal liver, during embryogenesis. Fluorescence-activated cell sorting and polymerase chain reaction analyses demonstrated that CD34+ cells, purified from the fetal liver, expressed additional HSC markers, c-Kit and Sca-1, and that these CD34+ cells, but not CD34− cells, highly expressed MFG-E8. We also found that MFG-E8 was not expressed in HSCs in adult mouse bone marrow, and that its expression was confined to F4/80+ macrophages. Together, this study demonstrates, for the first time, that MFG-8 is expressed in fetal HSC populations, and that MFG-E8 may have a role in embryonic hematopoiesis.
UR - http://www.scopus.com/inward/record.url?scp=85018543299&partnerID=8YFLogxK
U2 - 10.1038/EMM.2015.42
DO - 10.1038/EMM.2015.42
M3 - Article
C2 - 26206421
AN - SCOPUS:85018543299
SN - 1226-3613
VL - 47
JO - Experimental and Molecular Medicine
JF - Experimental and Molecular Medicine
IS - 7
M1 - e174
ER -