Field evaluation of carbon injection method for in-situ biological denitrification in groundwater using geochemical and metataxonomic analyses

This study focuses on the bioremediation of nitrate-contaminated groundwater, which has become a significant environmental problem due to the increasing usage of fertilizers and sewage disposal. The nitrate reduction efficiencies of biological denitrification by injection of carbon source in a pilot-scale treatment system setup were investigated at a groundwater contamination site. The field test was conducted using acetate as a carbon source for 22 days to assess the nitrate reduction efficiencies of in-situ treatment. Geochemical parameters and microbial community analysis using next-generation sequencing were performed before and after carbon source injection. After 12 h of reaction time, nitrate concentration decreased from 31.6 to 4.2 mg-N/L at PC-2, and then remained stable at 3.9 mg-N/L. The nitrate reduction rate when acetate was injected was 29.0 mg-N/L/day. Aquabacterium commune, pseudomonas brassicacearum, dechloromonas denitrificans, and Massilia FAOS were dominant species after acetate injection. Predictive metabolic pathway analysis indicated that nitrate reduction metabolisms during injection of acetate were denitrification and assimilatory nitrate reduction to ammonium. The evaluated hazard quotient of nitrate-contaminated groundwater significantly decreased after acetate injection (non-carcinogenic risk decreased from 1.176 to 0.134 for children). This research could provide fundamental information for decision-makers in nitrate-contaminated groundwater quality protection and management.