Field evaluation of transgenic poplars expressing the constitutively active small G protein for improved biomass traits

Na Young Kim; Hwi Seong Jeon; Myoung Hoon Lee; Ahra Cha; Dong Sook Lee; Hye Jung Lee; Hye Gi Kim; Hyoshin Lee; Young Im Choi; Ohkmae K. Park

doi:10.1016/j.biombioe.2017.12.015

Field evaluation of transgenic poplars expressing the constitutively active small G protein for improved biomass traits

Na Young Kim, Hwi Seong Jeon, Myoung Hoon Lee, Ahra Cha, Dong Sook Lee, Hye Jung Lee, Hye Gi Kim, Hyoshin Lee, Young Im Choi, Ohkmae K. Park

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

An Arabidopsis small G protein, RabG3b, has been previously characterized as a positive regulator of xylem development in Arabidopsis. Transgenic poplars overexpressing a constitutively active form of RabG3b (RabG3bCA) were developed, and their performance as potential biomass crops was evaluated in the field in Suwon, Korea (37°26′N, 126°98′E) from May to October 2016. RabG3bCA transgenic poplars showed increased stem growth and produced 3–4 times greater dry biomass than hybrid control poplars. Fiber length and diameter were also increased by 15–26% and 28–38%, respectively. Transgenic poplars were analyzed for enzymatic digestibility and biofuel production after chemical pretreatments. Glucose and ethanol yields were higher in pretreated transgenic poplars than hybrid control, and the pretreatment effectiveness of sodium hydroxide was better than that of the other chemicals. We propose that RabG3bCA can add value to the development of biomass crops.

Original language	English
Pages (from-to)	16-22
Number of pages	7
Journal	Biomass and Bioenergy
Volume	109
DOIs	https://doi.org/10.1016/j.biombioe.2017.12.015
Publication status	Published - 2018 Feb

Keywords

Arabidopsis
Biofuel
Biomass
RabG3b
Transgenic poplar
Xylem development

ASJC Scopus subject areas

Forestry
Renewable Energy, Sustainability and the Environment
Agronomy and Crop Science
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biombioe.2017.12.015

Cite this

@article{64c25341dc10474ba686fc88a6a334d2,

title = "Field evaluation of transgenic poplars expressing the constitutively active small G protein for improved biomass traits",

abstract = "An Arabidopsis small G protein, RabG3b, has been previously characterized as a positive regulator of xylem development in Arabidopsis. Transgenic poplars overexpressing a constitutively active form of RabG3b (RabG3bCA) were developed, and their performance as potential biomass crops was evaluated in the field in Suwon, Korea (37°26′N, 126°98′E) from May to October 2016. RabG3bCA transgenic poplars showed increased stem growth and produced 3–4 times greater dry biomass than hybrid control poplars. Fiber length and diameter were also increased by 15–26% and 28–38%, respectively. Transgenic poplars were analyzed for enzymatic digestibility and biofuel production after chemical pretreatments. Glucose and ethanol yields were higher in pretreated transgenic poplars than hybrid control, and the pretreatment effectiveness of sodium hydroxide was better than that of the other chemicals. We propose that RabG3bCA can add value to the development of biomass crops.",

keywords = "Arabidopsis, Biofuel, Biomass, RabG3b, Transgenic poplar, Xylem development",

author = "Kim, {Na Young} and Jeon, {Hwi Seong} and Lee, {Myoung Hoon} and Ahra Cha and Lee, {Dong Sook} and Lee, {Hye Jung} and Kim, {Hye Gi} and Hyoshin Lee and Choi, {Young Im} and Park, {Ohkmae K.}",

note = "Funding Information: This work was supported by a Korea University grant and the Next-Generation BioGreen 21 Programs (Plant Molecular Breeding Center, PJ011210) through the Rural Development Administration, and a National Research Foundation of Korea (NRF) grant funded by the Korean government (2017R1A2B4009991). Funding Information: This work was supported by a Korea University grant and the Next-Generation BioGreen 21 Programs ( Plant Molecular Breeding Center , PJ011210 ) through the Rural Development Administration , and a National Research Foundation of Korea (NRF) grant funded by the Korean government ( 2017R1A2B4009991 ). Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2018",

month = feb,

doi = "10.1016/j.biombioe.2017.12.015",

language = "English",

volume = "109",

pages = "16--22",

journal = "Biomass and Bioenergy",

issn = "0961-9534",

publisher = "Elsevier Limited",

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TY - JOUR

T1 - Field evaluation of transgenic poplars expressing the constitutively active small G protein for improved biomass traits

AU - Kim, Na Young

AU - Jeon, Hwi Seong

AU - Lee, Myoung Hoon

AU - Cha, Ahra

AU - Lee, Dong Sook

AU - Lee, Hye Jung

AU - Kim, Hye Gi

AU - Lee, Hyoshin

AU - Choi, Young Im

AU - Park, Ohkmae K.

N1 - Funding Information: This work was supported by a Korea University grant and the Next-Generation BioGreen 21 Programs (Plant Molecular Breeding Center, PJ011210) through the Rural Development Administration, and a National Research Foundation of Korea (NRF) grant funded by the Korean government (2017R1A2B4009991). Funding Information: This work was supported by a Korea University grant and the Next-Generation BioGreen 21 Programs ( Plant Molecular Breeding Center , PJ011210 ) through the Rural Development Administration , and a National Research Foundation of Korea (NRF) grant funded by the Korean government ( 2017R1A2B4009991 ). Publisher Copyright: © 2017 Elsevier Ltd

PY - 2018/2

Y1 - 2018/2

N2 - An Arabidopsis small G protein, RabG3b, has been previously characterized as a positive regulator of xylem development in Arabidopsis. Transgenic poplars overexpressing a constitutively active form of RabG3b (RabG3bCA) were developed, and their performance as potential biomass crops was evaluated in the field in Suwon, Korea (37°26′N, 126°98′E) from May to October 2016. RabG3bCA transgenic poplars showed increased stem growth and produced 3–4 times greater dry biomass than hybrid control poplars. Fiber length and diameter were also increased by 15–26% and 28–38%, respectively. Transgenic poplars were analyzed for enzymatic digestibility and biofuel production after chemical pretreatments. Glucose and ethanol yields were higher in pretreated transgenic poplars than hybrid control, and the pretreatment effectiveness of sodium hydroxide was better than that of the other chemicals. We propose that RabG3bCA can add value to the development of biomass crops.

AB - An Arabidopsis small G protein, RabG3b, has been previously characterized as a positive regulator of xylem development in Arabidopsis. Transgenic poplars overexpressing a constitutively active form of RabG3b (RabG3bCA) were developed, and their performance as potential biomass crops was evaluated in the field in Suwon, Korea (37°26′N, 126°98′E) from May to October 2016. RabG3bCA transgenic poplars showed increased stem growth and produced 3–4 times greater dry biomass than hybrid control poplars. Fiber length and diameter were also increased by 15–26% and 28–38%, respectively. Transgenic poplars were analyzed for enzymatic digestibility and biofuel production after chemical pretreatments. Glucose and ethanol yields were higher in pretreated transgenic poplars than hybrid control, and the pretreatment effectiveness of sodium hydroxide was better than that of the other chemicals. We propose that RabG3bCA can add value to the development of biomass crops.

KW - Arabidopsis

KW - Biofuel

KW - Biomass

KW - RabG3b

KW - Transgenic poplar

KW - Xylem development

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DO - 10.1016/j.biombioe.2017.12.015

M3 - Article

AN - SCOPUS:85039429377

SN - 0961-9534

VL - 109

SP - 16

EP - 22

JO - Biomass and Bioenergy

JF - Biomass and Bioenergy

ER -

Field evaluation of transgenic poplars expressing the constitutively active small G protein for improved biomass traits

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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