Flavobacterium crassostreae sp. nov., isolated from Pacific oyster

Sungmi Choi; Su Kyoung Shin; Eunji Kim; Hana Yi

doi:10.1099/ijsem.0.001728

Flavobacterium crassostreae sp. nov., isolated from Pacific oyster

Sungmi Choi, Su Kyoung Shin, Eunji Kim, Hana Yi

School of Biosystem and Biomedical Science

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A yellow, rod-shaped, Gram-reaction-negative bacterial strain, designated LPB0076^T, was isolated from a Pacific oyster. Results of 16S rRNA gene sequence analyses indicated that the strain represented a member of the genus Flavobacterium. It had the highest sequence similarity to the type strains of Flavobacterium frigidarium (97.6 %) and Flavobacterium omnivorum (97.0 %), and its similarities with all other species of the genus Flavobacterium were below 97.0 %. Its genome size (3.02 Mb), DNA G+C content (36.0 mol%), predominant cellular fatty acids (anteiso-C_{15 : 0}, iso-C_{15 : 0} and C_{16 : 1}ω7c and/or C_{17 : 1}ω6c), and major polar lipid (phosphatidylethanolamine) were similar to those described previously for members of the genus Flavobacterium. In contrast, a number of phenotypic characteristics, including the inability to grow microaerophilically, absence of flexirubin-type pigments and gliding motility and differences in enzymatic reactions, clearly distinguished LPB0076T from other species of the genus Flavobacterium. The polyphasic data presented in this study indicate that this isolate should be classified as representing a novel species of the genus Flavobacterium. The name Flavobacterium crassostreae sp. nov. is therefore proposed for the isolate, with the type strain being LPB0076^T (=KACC 18706^T=JCM 31219^T).

Original language	English
Article number	001728
Pages (from-to)	988-992
Number of pages	5
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	67
Issue number	4
DOIs	https://doi.org/10.1099/ijsem.0.001728
Publication status	Published - 2017 Apr

Bibliographical note

Funding Information:
This study was funded by the Survey of Korean Indigenous Species Program through the National Institute of Biological Resources funded by the Korean Ministry of Environment, the Strategic Initiative for Microbiomes in Agriculture and Food funded by the Ministry of Agriculture, Food and Rural Affairs (916006-2) and the Basic Science Research Programs through the National Research Foundation of Korea (NRF) funded by the Korean Ministry of Education (2015R1D1A1A01057527).

Publisher Copyright:
© 2017 IUMS.

Keywords

Bacteriodetes
Flavobacterium
Flavobacterium crassostreae

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics

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10.1099/ijsem.0.001728

Cite this

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title = "Flavobacterium crassostreae sp. nov., isolated from Pacific oyster",

abstract = "A yellow, rod-shaped, Gram-reaction-negative bacterial strain, designated LPB0076T, was isolated from a Pacific oyster. Results of 16S rRNA gene sequence analyses indicated that the strain represented a member of the genus Flavobacterium. It had the highest sequence similarity to the type strains of Flavobacterium frigidarium (97.6 %) and Flavobacterium omnivorum (97.0 %), and its similarities with all other species of the genus Flavobacterium were below 97.0 %. Its genome size (3.02 Mb), DNA G+C content (36.0 mol%), predominant cellular fatty acids (anteiso-C15 : 0, iso-C15 : 0 and C16 : 1ω7c and/or C17 : 1ω6c), and major polar lipid (phosphatidylethanolamine) were similar to those described previously for members of the genus Flavobacterium. In contrast, a number of phenotypic characteristics, including the inability to grow microaerophilically, absence of flexirubin-type pigments and gliding motility and differences in enzymatic reactions, clearly distinguished LPB0076T from other species of the genus Flavobacterium. The polyphasic data presented in this study indicate that this isolate should be classified as representing a novel species of the genus Flavobacterium. The name Flavobacterium crassostreae sp. nov. is therefore proposed for the isolate, with the type strain being LPB0076T (=KACC 18706T=JCM 31219T).",

keywords = "Bacteriodetes, Flavobacterium, Flavobacterium crassostreae",

author = "Sungmi Choi and Shin, {Su Kyoung} and Eunji Kim and Hana Yi",

note = "Funding Information: This study was funded by the Survey of Korean Indigenous Species Program through the National Institute of Biological Resources funded by the Korean Ministry of Environment, the Strategic Initiative for Microbiomes in Agriculture and Food funded by the Ministry of Agriculture, Food and Rural Affairs (916006-2) and the Basic Science Research Programs through the National Research Foundation of Korea (NRF) funded by the Korean Ministry of Education (2015R1D1A1A01057527). Publisher Copyright: {\textcopyright} 2017 IUMS.",

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doi = "10.1099/ijsem.0.001728",

language = "English",

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pages = "988--992",

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AU - Choi, Sungmi

AU - Shin, Su Kyoung

AU - Kim, Eunji

AU - Yi, Hana

N1 - Funding Information: This study was funded by the Survey of Korean Indigenous Species Program through the National Institute of Biological Resources funded by the Korean Ministry of Environment, the Strategic Initiative for Microbiomes in Agriculture and Food funded by the Ministry of Agriculture, Food and Rural Affairs (916006-2) and the Basic Science Research Programs through the National Research Foundation of Korea (NRF) funded by the Korean Ministry of Education (2015R1D1A1A01057527). Publisher Copyright: © 2017 IUMS.

PY - 2017/4

Y1 - 2017/4

N2 - A yellow, rod-shaped, Gram-reaction-negative bacterial strain, designated LPB0076T, was isolated from a Pacific oyster. Results of 16S rRNA gene sequence analyses indicated that the strain represented a member of the genus Flavobacterium. It had the highest sequence similarity to the type strains of Flavobacterium frigidarium (97.6 %) and Flavobacterium omnivorum (97.0 %), and its similarities with all other species of the genus Flavobacterium were below 97.0 %. Its genome size (3.02 Mb), DNA G+C content (36.0 mol%), predominant cellular fatty acids (anteiso-C15 : 0, iso-C15 : 0 and C16 : 1ω7c and/or C17 : 1ω6c), and major polar lipid (phosphatidylethanolamine) were similar to those described previously for members of the genus Flavobacterium. In contrast, a number of phenotypic characteristics, including the inability to grow microaerophilically, absence of flexirubin-type pigments and gliding motility and differences in enzymatic reactions, clearly distinguished LPB0076T from other species of the genus Flavobacterium. The polyphasic data presented in this study indicate that this isolate should be classified as representing a novel species of the genus Flavobacterium. The name Flavobacterium crassostreae sp. nov. is therefore proposed for the isolate, with the type strain being LPB0076T (=KACC 18706T=JCM 31219T).

AB - A yellow, rod-shaped, Gram-reaction-negative bacterial strain, designated LPB0076T, was isolated from a Pacific oyster. Results of 16S rRNA gene sequence analyses indicated that the strain represented a member of the genus Flavobacterium. It had the highest sequence similarity to the type strains of Flavobacterium frigidarium (97.6 %) and Flavobacterium omnivorum (97.0 %), and its similarities with all other species of the genus Flavobacterium were below 97.0 %. Its genome size (3.02 Mb), DNA G+C content (36.0 mol%), predominant cellular fatty acids (anteiso-C15 : 0, iso-C15 : 0 and C16 : 1ω7c and/or C17 : 1ω6c), and major polar lipid (phosphatidylethanolamine) were similar to those described previously for members of the genus Flavobacterium. In contrast, a number of phenotypic characteristics, including the inability to grow microaerophilically, absence of flexirubin-type pigments and gliding motility and differences in enzymatic reactions, clearly distinguished LPB0076T from other species of the genus Flavobacterium. The polyphasic data presented in this study indicate that this isolate should be classified as representing a novel species of the genus Flavobacterium. The name Flavobacterium crassostreae sp. nov. is therefore proposed for the isolate, with the type strain being LPB0076T (=KACC 18706T=JCM 31219T).

KW - Bacteriodetes

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EP - 992

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

IS - 4

M1 - 001728

ER -

Flavobacterium crassostreae sp. nov., isolated from Pacific oyster

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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