Flavobacterium limnosediminis sp. nov., isolated from sediment of a freshwater lake

A Gram-stain-negative, rod-shaped, pale yellow, aerobic bacterial strain, JC2902^T, was isolated from a sediment sample of Ungok Lake in Gochang, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain JC2902^T belongs to the genus Flavobacterium and forms a distinct phyletic line within a clade containing four recognized species of the genus Flavobacterium. The genomic relatedness between strain JC2902^T and closely related strains was calculated using average nucleotide identity values of whole genome sequences, which indicated that the new isolate represents a novel genomic species. Through comparison of chemotaxonomic and other phenotypic characteristics between strain JC2902^T and the type strains of the four phylogenetically related species, a number of characteristics differentiated strain JC2902^T from the previously described type strains. Differential characteristics of strain JC2902^T include fatty acid profiles, cellular motility, inability to grow on Luria-Bertani and tripticase soy agar media, and absence of N-acetyl-β-glucosaminidase and flexirubin-type pigments. Based on data from this polyphasic taxonomic study, strain JC2902^T is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium limnosediminis sp. nov. is proposed. The type strain is JC2902^T (= KACC 16937^T = JCM 18661^T).