Flexible-type ultrathin holographic endoscope for microscopic imaging of unstained biological tissues

Wonjun Choi; Munkyu Kang; Jin Hee Hong; Ori Katz; Byunghak Lee; Guang Hoon Kim; Youngwoon Choi; Wonshik Choi

doi:10.1038/s41467-022-32114-5

Flexible-type ultrathin holographic endoscope for microscopic imaging of unstained biological tissues

Wonjun Choi, Munkyu Kang, Jin Hee Hong, Ori Katz, Byunghak Lee, Guang Hoon Kim, Youngwoon Choi, Wonshik Choi

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Ultrathin lensless fibre endoscopes offer minimally invasive investigation, but they mostly operate as a rigid type due to the need for prior calibration of a fibre probe. Furthermore, most implementations work in fluorescence mode rather than label-free imaging mode, making them unsuitable for general medical diagnosis. Herein, we report a fully flexible ultrathin fibre endoscope taking 3D holographic images of unstained tissues with 0.85-μm spatial resolution. Using a bare fibre bundle as thin as 200-μm diameter, we design a lensless Fourier holographic imaging configuration to selectively detect weak reflections from biological tissues, a critical step for label-free endoscopic reflectance imaging. A unique algorithm is developed for calibration-free holographic image reconstruction, allowing us to image through a narrow and curved passage regardless of fibre bending. We demonstrate endoscopic reflectance imaging of unstained rat intestine tissues that are completely invisible to conventional endoscopes. The proposed endoscope will expedite a more accurate and earlier diagnosis than before with minimal complications.

Original language	English
Article number	4469
Journal	Nature communications
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41467-022-32114-5
Publication status	Published - 2022 Dec

Bibliographical note

Funding Information:
This research was supported by IBS-R023-D1, the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. 2021R1A2C2012069), European Research Council (ERC) Horizon 2020 research and innovation program (grants no. 677909, 101002406), and Israel Science Foundation (1361/18).

Publisher Copyright:
© 2022, The Author(s).

ASJC Scopus subject areas

Physics and Astronomy(all)
Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)

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10.1038/s41467-022-32114-5

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title = "Flexible-type ultrathin holographic endoscope for microscopic imaging of unstained biological tissues",

abstract = "Ultrathin lensless fibre endoscopes offer minimally invasive investigation, but they mostly operate as a rigid type due to the need for prior calibration of a fibre probe. Furthermore, most implementations work in fluorescence mode rather than label-free imaging mode, making them unsuitable for general medical diagnosis. Herein, we report a fully flexible ultrathin fibre endoscope taking 3D holographic images of unstained tissues with 0.85-μm spatial resolution. Using a bare fibre bundle as thin as 200-μm diameter, we design a lensless Fourier holographic imaging configuration to selectively detect weak reflections from biological tissues, a critical step for label-free endoscopic reflectance imaging. A unique algorithm is developed for calibration-free holographic image reconstruction, allowing us to image through a narrow and curved passage regardless of fibre bending. We demonstrate endoscopic reflectance imaging of unstained rat intestine tissues that are completely invisible to conventional endoscopes. The proposed endoscope will expedite a more accurate and earlier diagnosis than before with minimal complications.",

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AU - Kim, Guang Hoon

AU - Choi, Youngwoon

AU - Choi, Wonshik

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Flexible-type ultrathin holographic endoscope for microscopic imaging of unstained biological tissues

Abstract

Bibliographical note

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