TY - JOUR
T1 - Fluorescence enhancement of N 2O 2-type dipyrrin ligand in two step responding to zinc(II) ion
AU - Song, Hyesun
AU - Rajendiran, Senkuttuvan
AU - Koo, Eunhae
AU - Min, Byoung Koun
AU - Jeong, Soon Kwan
AU - Daniel Thangadurai, T.
AU - Yoon, Sungho
N1 - Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (MEST) ( KRF-2008-C00146 ), by the Industrial Technology Development Program funded by the Ministry of Knowledge Economy (MKE, Korea), and also by a grant from the Fundamental R&D program for Core Technology of Materials funded by the Ministry of Knowledge Economy, Republic of Korea.
PY - 2012/11
Y1 - 2012/11
N2 - Fluorescence imaging is well-suited for the live imaging of biological Zn(II), which has no facile spectroscopic or magnetic signature. The successful application of this methodology requires the development of robust Zn(II) imaging agents that display high sensitivity, selectivity and temporal fidelity. In this study, a N 2O 2-type dipyrrin based bimolecular zinc(II) complex was produced and shown to have sharper, blue-shifted and more enhanced fluorescence emission. An approximate three-fold fluorescence enhancement was achieved within the micromolar concentration range, which is an important parameter for Zn(II) detection in vivo. The increase in emission intensity was due to the dominant role of aryl-ring rotation in governing the excited state dynamics and fluorescence properties of the dipyrrin dye. Fluorescence titration showed that the ligand complex exhibited very strong zinc(II) binding affinity when compared to that in the binuclear chloro complex. The fluorescence emission changes in the dipyrrin dye to zinc(II) ion could be observed not only using instruments but also by the naked-eye (violet→sky blue).
AB - Fluorescence imaging is well-suited for the live imaging of biological Zn(II), which has no facile spectroscopic or magnetic signature. The successful application of this methodology requires the development of robust Zn(II) imaging agents that display high sensitivity, selectivity and temporal fidelity. In this study, a N 2O 2-type dipyrrin based bimolecular zinc(II) complex was produced and shown to have sharper, blue-shifted and more enhanced fluorescence emission. An approximate three-fold fluorescence enhancement was achieved within the micromolar concentration range, which is an important parameter for Zn(II) detection in vivo. The increase in emission intensity was due to the dominant role of aryl-ring rotation in governing the excited state dynamics and fluorescence properties of the dipyrrin dye. Fluorescence titration showed that the ligand complex exhibited very strong zinc(II) binding affinity when compared to that in the binuclear chloro complex. The fluorescence emission changes in the dipyrrin dye to zinc(II) ion could be observed not only using instruments but also by the naked-eye (violet→sky blue).
KW - Detection limit
KW - Dipyrrin
KW - Fluorescence enhancement
KW - Ligand complex
KW - Zinc(II)
UR - http://www.scopus.com/inward/record.url?scp=84864026414&partnerID=8YFLogxK
U2 - 10.1016/j.jlumin.2012.06.024
DO - 10.1016/j.jlumin.2012.06.024
M3 - Article
AN - SCOPUS:84864026414
SN - 0022-2313
VL - 132
SP - 3089
EP - 3092
JO - Journal of Luminescence
JF - Journal of Luminescence
IS - 11
ER -