Fluorescence switch for silver ion detection utilizing dimerization of DNA-Ag nanoclusters

Jihyun Lee; Juhee Park; Hong Hee Lee; Hansoo Park; Hugh I. Kim; Won Jong Kim

doi:10.1016/j.bios.2015.01.058

Fluorescence switch for silver ion detection utilizing dimerization of DNA-Ag nanoclusters

Jihyun Lee, Juhee Park, Hong Hee Lee, Hansoo Park, Hugh I. Kim, Won Jong Kim

Research output: Contribution to journal › Article › peer-review

78 Citations (Scopus)

Abstract

A fluorescence switch that consists of DNA-templated silver nanoclusters (DNA-AgNCs) triggered by silver ion (Ag⁺) is developed to detect Ag⁺. The mechanism of the fluorescence switching of DNA-AgNCs is investigated by fluorescence spectroscopy, circular dichroism spectroscopy, DNA hybridization assay and mass spectrometry. Ag⁺ induces a dimeric structure of Cyt₁₂-AgNCs by forming a bridge between two Cyt₁₂-AgNCs, where Cyt₁₂ is cytosine 12-mer; this dimer formation causes the fluorescence change of Cyt₁₂-AgNCs from red to green. Using this Ag⁺-triggered fluorescence switch, we successfully detected Ag⁺ at concentrations as low as 10nM. Furthermore, we quantitatively detected the Ag⁺ in the Silmazin^®, which is dermatological burn ointment having silver sulfadiazine. Ag⁺ detection using this fluorescence switch has high selectivity and sensitivity, and short response time, and can be used successfully even in the presence of other metal ions.

Original language	English
Pages (from-to)	642-647
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	68
DOIs	https://doi.org/10.1016/j.bios.2015.01.058
Publication status	Published - 2015 Jun 5
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Research Center Program of IBS (Institute for Basic Science) in Korea ( CA1203-02 ). Jihyun Lee was supported by Global Ph.D Fellowship grant from National Research Foundation (NRF) funded by the Ministry of Education (NRF-2012-H1A2A1005715).

Publisher Copyright:
© 2015 Elsevier B.V.

Keywords

DNA-template silver nanoclusters
Fluorescence switch
Fluorescent probes
Silver ion detection

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2015.01.058

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title = "Fluorescence switch for silver ion detection utilizing dimerization of DNA-Ag nanoclusters",

abstract = "A fluorescence switch that consists of DNA-templated silver nanoclusters (DNA-AgNCs) triggered by silver ion (Ag+) is developed to detect Ag+. The mechanism of the fluorescence switching of DNA-AgNCs is investigated by fluorescence spectroscopy, circular dichroism spectroscopy, DNA hybridization assay and mass spectrometry. Ag+ induces a dimeric structure of Cyt12-AgNCs by forming a bridge between two Cyt12-AgNCs, where Cyt12 is cytosine 12-mer; this dimer formation causes the fluorescence change of Cyt12-AgNCs from red to green. Using this Ag+-triggered fluorescence switch, we successfully detected Ag+ at concentrations as low as 10nM. Furthermore, we quantitatively detected the Ag+ in the Silmazin{\textregistered}, which is dermatological burn ointment having silver sulfadiazine. Ag+ detection using this fluorescence switch has high selectivity and sensitivity, and short response time, and can be used successfully even in the presence of other metal ions.",

keywords = "DNA-template silver nanoclusters, Fluorescence switch, Fluorescent probes, Silver ion detection",

author = "Jihyun Lee and Juhee Park and {Hee Lee}, Hong and Hansoo Park and Kim, {Hugh I.} and Kim, {Won Jong}",

note = "Funding Information: This work was supported by the Research Center Program of IBS (Institute for Basic Science) in Korea ( CA1203-02 ). Jihyun Lee was supported by Global Ph.D Fellowship grant from National Research Foundation (NRF) funded by the Ministry of Education (NRF-2012-H1A2A1005715). Publisher Copyright: {\textcopyright} 2015 Elsevier B.V.",

year = "2015",

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doi = "10.1016/j.bios.2015.01.058",

language = "English",

volume = "68",

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AU - Lee, Jihyun

AU - Park, Juhee

AU - Hee Lee, Hong

AU - Park, Hansoo

AU - Kim, Hugh I.

AU - Kim, Won Jong

N1 - Funding Information: This work was supported by the Research Center Program of IBS (Institute for Basic Science) in Korea ( CA1203-02 ). Jihyun Lee was supported by Global Ph.D Fellowship grant from National Research Foundation (NRF) funded by the Ministry of Education (NRF-2012-H1A2A1005715). Publisher Copyright: © 2015 Elsevier B.V.

PY - 2015/6/5

Y1 - 2015/6/5

N2 - A fluorescence switch that consists of DNA-templated silver nanoclusters (DNA-AgNCs) triggered by silver ion (Ag+) is developed to detect Ag+. The mechanism of the fluorescence switching of DNA-AgNCs is investigated by fluorescence spectroscopy, circular dichroism spectroscopy, DNA hybridization assay and mass spectrometry. Ag+ induces a dimeric structure of Cyt12-AgNCs by forming a bridge between two Cyt12-AgNCs, where Cyt12 is cytosine 12-mer; this dimer formation causes the fluorescence change of Cyt12-AgNCs from red to green. Using this Ag+-triggered fluorescence switch, we successfully detected Ag+ at concentrations as low as 10nM. Furthermore, we quantitatively detected the Ag+ in the Silmazin®, which is dermatological burn ointment having silver sulfadiazine. Ag+ detection using this fluorescence switch has high selectivity and sensitivity, and short response time, and can be used successfully even in the presence of other metal ions.

AB - A fluorescence switch that consists of DNA-templated silver nanoclusters (DNA-AgNCs) triggered by silver ion (Ag+) is developed to detect Ag+. The mechanism of the fluorescence switching of DNA-AgNCs is investigated by fluorescence spectroscopy, circular dichroism spectroscopy, DNA hybridization assay and mass spectrometry. Ag+ induces a dimeric structure of Cyt12-AgNCs by forming a bridge between two Cyt12-AgNCs, where Cyt12 is cytosine 12-mer; this dimer formation causes the fluorescence change of Cyt12-AgNCs from red to green. Using this Ag+-triggered fluorescence switch, we successfully detected Ag+ at concentrations as low as 10nM. Furthermore, we quantitatively detected the Ag+ in the Silmazin®, which is dermatological burn ointment having silver sulfadiazine. Ag+ detection using this fluorescence switch has high selectivity and sensitivity, and short response time, and can be used successfully even in the presence of other metal ions.

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Fluorescence switch for silver ion detection utilizing dimerization of DNA-Ag nanoclusters

Abstract

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