TY - JOUR
T1 - Folate-modified-chitosan-coated liposomes for tumor-targeted drug delivery
AU - Yang, Kui Kun
AU - Kong, Ming
AU - Wei, Ya Nan
AU - Liu, Ya
AU - Cheng, Xiao Jie
AU - Li, Jing
AU - Park, Hyun Jin
AU - Chen, Xi Guang
N1 - Funding Information:
Acknowledgements This research was granted by Science and Technology Breakthrough Project (2011GSF12124), National Natural Science Foundation of China (81071274) and International Science and Technology Cooperation Project (2011DFA31270).
PY - 2013/2
Y1 - 2013/2
N2 - In this study, a folate-modified-chitosan-coated liposome (FCCL) was prepared for tumor-targeted drug delivery. The folate-chitosan conjugates were characterized using 1H NMR and infrared spectrum analysis. The properties of folate-chitosan-coated liposomes (FCCLs) were studied and compared with those of traditional liposomes and chitosan-coated liposomes (CCLs). FCCLs were spherical in shape with a classic core-shell structure. Compared with conventional liposomes, FCCLs had larger size (average diameter: 182.0 nm), higher zeta potential (10.1 mV), prolonged drug release behaviors (55.76 % after 24 h), and better physical stability when stored at 25 C, all similar to the properties of CCLs. With fluorescein as a model drug, fluorescein-loaded liposomes, CCLs, and FCCLs were prepared and their tumor targeting ability was evaluated according to the in vitro cellular uptake of fluorescein loaded nanoparticles by MCF-7 and HUVEC cells. Results demonstrated that FCCLs had a significant higher uptake by folate receptor positive cells (MCF-7) as compared to traditional liposomes and CCLs, which indicated that the FCCLs were promising nano-carriers for tumor-targeted drug delivery.
AB - In this study, a folate-modified-chitosan-coated liposome (FCCL) was prepared for tumor-targeted drug delivery. The folate-chitosan conjugates were characterized using 1H NMR and infrared spectrum analysis. The properties of folate-chitosan-coated liposomes (FCCLs) were studied and compared with those of traditional liposomes and chitosan-coated liposomes (CCLs). FCCLs were spherical in shape with a classic core-shell structure. Compared with conventional liposomes, FCCLs had larger size (average diameter: 182.0 nm), higher zeta potential (10.1 mV), prolonged drug release behaviors (55.76 % after 24 h), and better physical stability when stored at 25 C, all similar to the properties of CCLs. With fluorescein as a model drug, fluorescein-loaded liposomes, CCLs, and FCCLs were prepared and their tumor targeting ability was evaluated according to the in vitro cellular uptake of fluorescein loaded nanoparticles by MCF-7 and HUVEC cells. Results demonstrated that FCCLs had a significant higher uptake by folate receptor positive cells (MCF-7) as compared to traditional liposomes and CCLs, which indicated that the FCCLs were promising nano-carriers for tumor-targeted drug delivery.
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U2 - 10.1007/s10853-012-6930-0
DO - 10.1007/s10853-012-6930-0
M3 - Article
AN - SCOPUS:84871808780
SN - 0022-2461
VL - 48
SP - 1717
EP - 1728
JO - Journal of Materials Science
JF - Journal of Materials Science
IS - 4
ER -