Formation of a dynamic membrane altered the microbial community and metabolic flux in fermentative hydrogen production

Jong Hun Park; Jeong Hoon Park; Young Bo Sim; Sang Hyoun Kim; Hee Deung Park

doi:10.1016/j.biortech.2019.02.124

Formation of a dynamic membrane altered the microbial community and metabolic flux in fermentative hydrogen production

Jong Hun Park
, Jeong Hoon Park
, Young Bo Sim
, Sang Hyoun Kim
, Hee Deung Park^*

^*Corresponding author for this work

School of Civil, Environmental and Architectural Engineering

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

This study aimed to investigate the relationship among dynamic membrane (DM) formation, metabolic flux, and microbial community population in dark fermentative hydrogen production. A continuously stirred tank reactor was equipped with an external submerged polyester screen mesh and inoculated with heat-treated anaerobic sludge without immobilization. DM was successfully developed on the polyester mesh and provided high-rate hydrogen production at 60.5 L H₂/L.d and 2.39 mol H₂/mol glucose_added. DM formation was along with tightly bound extracellular polymeric substances. Flux balance analysis revealed that formation of DM altered the metabolic pathways for acetic acid production from homoacetogenesis to hydrogenesis. Bacterial community analysis suggested that Sporolactobacillaceae would contributed to this metabolic pathway shift. Nevertheless, lactic acid was not accumulated and assumed to be consumed by hydrogen producers including Clostridia.

Original language	English
Pages (from-to)	63-68
Number of pages	6
Journal	Bioresource technology
Volume	282
DOIs	https://doi.org/10.1016/j.biortech.2019.02.124
Publication status	Published - 2019 Jun

Bibliographical note

Publisher Copyright:
© 2019 Elsevier Ltd

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Biofilm
Dynamic membrane
Flux balance analysis
High-rate bio-H
Sporolactobacillaceae

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

Access to Document

10.1016/j.biortech.2019.02.124

Cite this

@article{0bb136211cf645788f43b2cfae2dd66f,

title = "Formation of a dynamic membrane altered the microbial community and metabolic flux in fermentative hydrogen production",

abstract = "This study aimed to investigate the relationship among dynamic membrane (DM) formation, metabolic flux, and microbial community population in dark fermentative hydrogen production. A continuously stirred tank reactor was equipped with an external submerged polyester screen mesh and inoculated with heat-treated anaerobic sludge without immobilization. DM was successfully developed on the polyester mesh and provided high-rate hydrogen production at 60.5 L H2/L.d and 2.39 mol H2/mol glucoseadded. DM formation was along with tightly bound extracellular polymeric substances. Flux balance analysis revealed that formation of DM altered the metabolic pathways for acetic acid production from homoacetogenesis to hydrogenesis. Bacterial community analysis suggested that Sporolactobacillaceae would contributed to this metabolic pathway shift. Nevertheless, lactic acid was not accumulated and assumed to be consumed by hydrogen producers including Clostridia.",

keywords = "Biofilm, Dynamic membrane, Flux balance analysis, High-rate bio-H, Sporolactobacillaceae",

author = "Park, \{Jong Hun\} and Park, \{Jeong Hoon\} and Sim, \{Young Bo\} and Kim, \{Sang Hyoun\} and Park, \{Hee Deung\}",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2019",

month = jun,

doi = "10.1016/j.biortech.2019.02.124",

language = "English",

volume = "282",

pages = "63--68",

journal = "Bioresource technology",

issn = "0960-8524",

publisher = "Elsevier Ltd",

}

TY - JOUR

T1 - Formation of a dynamic membrane altered the microbial community and metabolic flux in fermentative hydrogen production

AU - Park, Jong Hun

AU - Park, Jeong Hoon

AU - Sim, Young Bo

AU - Kim, Sang Hyoun

AU - Park, Hee Deung

PY - 2019/6

Y1 - 2019/6

N2 - This study aimed to investigate the relationship among dynamic membrane (DM) formation, metabolic flux, and microbial community population in dark fermentative hydrogen production. A continuously stirred tank reactor was equipped with an external submerged polyester screen mesh and inoculated with heat-treated anaerobic sludge without immobilization. DM was successfully developed on the polyester mesh and provided high-rate hydrogen production at 60.5 L H2/L.d and 2.39 mol H2/mol glucoseadded. DM formation was along with tightly bound extracellular polymeric substances. Flux balance analysis revealed that formation of DM altered the metabolic pathways for acetic acid production from homoacetogenesis to hydrogenesis. Bacterial community analysis suggested that Sporolactobacillaceae would contributed to this metabolic pathway shift. Nevertheless, lactic acid was not accumulated and assumed to be consumed by hydrogen producers including Clostridia.

AB - This study aimed to investigate the relationship among dynamic membrane (DM) formation, metabolic flux, and microbial community population in dark fermentative hydrogen production. A continuously stirred tank reactor was equipped with an external submerged polyester screen mesh and inoculated with heat-treated anaerobic sludge without immobilization. DM was successfully developed on the polyester mesh and provided high-rate hydrogen production at 60.5 L H2/L.d and 2.39 mol H2/mol glucoseadded. DM formation was along with tightly bound extracellular polymeric substances. Flux balance analysis revealed that formation of DM altered the metabolic pathways for acetic acid production from homoacetogenesis to hydrogenesis. Bacterial community analysis suggested that Sporolactobacillaceae would contributed to this metabolic pathway shift. Nevertheless, lactic acid was not accumulated and assumed to be consumed by hydrogen producers including Clostridia.

KW - Biofilm

KW - Dynamic membrane

KW - Flux balance analysis

KW - High-rate bio-H

KW - Sporolactobacillaceae

UR - https://www.scopus.com/pages/publications/85062400870

U2 - 10.1016/j.biortech.2019.02.124

DO - 10.1016/j.biortech.2019.02.124

M3 - Article

C2 - 30851575

AN - SCOPUS:85062400870

SN - 0960-8524

VL - 282

SP - 63

EP - 68

JO - Bioresource technology

JF - Bioresource technology

ER -

Formation of a dynamic membrane altered the microbial community and metabolic flux in fermentative hydrogen production

Abstract

Bibliographical note

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this