@article{27701098d0e8402bb2deb17ef93d9fad,
title = "Formation of cellular close-ended tunneling nanotubes through mechanical deformation",
abstract = "Membrane nanotubes or tunneling nanotubes (TNTs) that connect cells have been recognized as a previously unidentified pathway for intercellular transport between distant cells. However, it is unknown how this delicate structure, which extends over tens of micrometers and remains robust for hours, is formed. Here, we found that a TNT develops from a double filopodial bridge (DFB) created by the physical contact of two filopodia through helical deformation of the DFB. The transition of a DFB to a close-ended TNT is most likely triggered by disruption of the adhesion of two filopodia by mechanical energy accumulated in a twisted DFB when one of the DFB ends is firmly attached through intercellular cadherin-cadherin interactions. These studies pinpoint the mechanistic questions about TNTs and elucidate a formation mechanism.",
author = "Minhyeok Chang and Lee, {O. Chul} and Gayun Bu and Jaeho Oh and Yunn, {Na Oh} and Ryu, {Sung Ho} and Kwon, {Hyung Bae} and Kolomeisky, {Anatoly B.} and Shim, {Sang Hee} and Junsang Doh and Jeon, {Jae Hyung} and Lee, {Jong Bong}",
note = "Funding Information: Funding: This research was supported by the National Research Foundation of Korea funded by the Ministry of Science and ICT (2021R1I1A1A01059649 to O.-c.L., 2020R1A2C4002490 to J.-H.J., and 2017K1A1A2013241 to J.-B.L.) and LG Yonam Foundation of Korea (to J.-B.L.). This work was also supported by the National Institutes of Health grant DP1MH119428 (to H.-B.K.). We are grateful for the support of POSTECH Basic Research Science Center. Author contributions: M.C. and J.-B.L. conceived the project. M.C., G.B., and J.O. performed all the experiments. O.-c.L. and J.-H.J. performed the computer-aided simulation and developed the theoretical model. N.-O.Y. and S.H.R. helped cell cultures and provided materials. H.-B.K. and J.D. contributed to critical discussion and provided materials. A.B.K. and S.-H.S. contributed to data analysis and critical discussion. M.C., O.-c.L., G.B., J.-H.J., and J.-B.L. wrote the paper. All authors contributed to data analysis and edited the manuscript. Competing interests: The authors declare that they have no competing interests. Data and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2022",
month = apr,
doi = "10.1126/sciadv.abj3995",
language = "English",
volume = "8",
journal = "Science Advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "13",
}