Fugetaxis of Cell-in-Catalytic-Coat Nanobiohybrids in Glucose Gradients

Hyeong Bin Rheem, Hyunwoo Choi, Seoin Yang, Sol Han, Su Yeon Rhee, Hyeongseop Jeong, Kyung Bok Lee, Yeji Lee, In San Kim, Hojae Lee, Insung S. Choi

Research output: Contribution to journalArticlepeer-review

Abstract

Manipulation and control of cell chemotaxis remain an underexplored territory despite vast potential in various fields, such as cytotherapeutics, sensors, and even cell robots. Herein is achieved the chemical control over chemotactic movement and direction of Jurkat T cells, as a representative model, by the construction of cell-in-catalytic-coat structures in single-cell nanoencapsulation. Armed with the catalytic power of glucose oxidase (GOx) in the artificial coat, the nanobiohybrid cytostructures, denoted as Jurkat[Lipo_GOx], exhibit controllable, redirected chemotactic movement in response to d-glucose gradients, in the opposite direction to the positive-chemotaxis direction of naïve, uncoated Jurkat cells in the same gradients. The chemically endowed, reaction-based fugetaxis of Jurkat[Lipo_GOx] operates orthogonally and complementarily to the endogenous, binding/recognition-based chemotaxis that remains intact after the formation of a GOx coat. For instance, the chemotactic velocity of Jurkat[Lipo_GOx] can be adjusted by varying the combination of d-glucose and natural chemokines (CXCL12 and CCL19) in the gradient. This work offers an innovative chemical tool for bioaugmenting living cells at the single-cell level through the use of catalytic cell-in-coat structures.

Original languageEnglish
Article number2301431
JournalSmall
Volume19
Issue number41
DOIs
Publication statusPublished - 2023 Oct 11

Bibliographical note

Publisher Copyright:
© 2023 Wiley-VCH GmbH.

Keywords

  • catalysis
  • chemokines
  • chemotaxis
  • liposomes
  • single-cell nanoencapsulation

ASJC Scopus subject areas

  • Biotechnology
  • General Chemistry
  • Biomaterials
  • General Materials Science
  • Engineering (miscellaneous)

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