Functional conservation and diversification between rice OsMADS22/OsMADS55 and Arabidopsis SVP proteins

Jeong Hwan Lee; Soo Hyun Park; Ji Hoon Ahn

doi:10.1016/j.plantsci.2011.09.003

Functional conservation and diversification between rice OsMADS22/OsMADS55 and Arabidopsis SVP proteins

Jeong Hwan Lee, Soo Hyun Park, Ji Hoon Ahn

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48 Citations (Scopus)

Abstract

MADS-box transcription factors play pivotal roles in several aspects of plant growth and development. The Arabidopsis SHORT VEGETATIVE PHASE (SVP) protein mediates the integration of signals involved in the control of flowering time and flower development by interacting with MADS-box proteins. In the rice genome, three SVP-like genes (OsMADS22, OsMADS47, and OsMADS55) are present. To investigate the functional conservation of these SVP-like genes in rice and Arabidopsis, the phenotypes of transgenic Arabidopsis plants overexpressing OsMADS22 and OsMADS55 were analyzed. Overexpression of OsMADS22 and OsMADS55 led to abnormal floral morphologies including leaf-like sepals, whereas only OsMADS55 expression caused delayed flowering via downregulation of FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1). Yeast two-hybrid assays revealed that OsMADS22 and OsMADS55 interacted with Arabidopsis AGL24 and AP1, but only OsMADS55 interacted with FLC. Overexpression of OsMADS55, but not OsMADS22, complemented the early flowering phenotype and ambient temperature-insensitive flowering phenotype seen in svp mutants, suggesting that OsMADS55 regulates flowering time associated with ambient temperature responses in Arabidopsis. Taken together, our data are consistent with functional conservation and diversification between Arabidopsis and rice SVP-like genes involved in controlling flowering time and flower development.

Original language	English
Pages (from-to)	97-104
Number of pages	8
Journal	Plant Science
Volume	185-186
DOIs	https://doi.org/10.1016/j.plantsci.2011.09.003
Publication status	Published - 2012 Apr

Bibliographical note

Funding Information:
We thank Dr. Jeong Kook Kim for RNA materials and helpful discussion. Also, we thank H. Sommer for providing the 35S::SVP seeds used in this study. This work was supported by the Creative Research Initiatives of the National Research Foundation for the Ministry of Education, Science and Technology ( R16-2008-106–01000-0 to J.H.A.), by the Korea University Grant (to J.H.A. and J.H.L.), and by the Brain Korea 21 program (to J.H.L. and S.H.P.).

Keywords

Flower development
Flowering time
OsMADS22
OsMADS55
Rice
SVP

ASJC Scopus subject areas

Genetics
Agronomy and Crop Science
Plant Science

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10.1016/j.plantsci.2011.09.003

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title = "Functional conservation and diversification between rice OsMADS22/OsMADS55 and Arabidopsis SVP proteins",

abstract = "MADS-box transcription factors play pivotal roles in several aspects of plant growth and development. The Arabidopsis SHORT VEGETATIVE PHASE (SVP) protein mediates the integration of signals involved in the control of flowering time and flower development by interacting with MADS-box proteins. In the rice genome, three SVP-like genes (OsMADS22, OsMADS47, and OsMADS55) are present. To investigate the functional conservation of these SVP-like genes in rice and Arabidopsis, the phenotypes of transgenic Arabidopsis plants overexpressing OsMADS22 and OsMADS55 were analyzed. Overexpression of OsMADS22 and OsMADS55 led to abnormal floral morphologies including leaf-like sepals, whereas only OsMADS55 expression caused delayed flowering via downregulation of FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1). Yeast two-hybrid assays revealed that OsMADS22 and OsMADS55 interacted with Arabidopsis AGL24 and AP1, but only OsMADS55 interacted with FLC. Overexpression of OsMADS55, but not OsMADS22, complemented the early flowering phenotype and ambient temperature-insensitive flowering phenotype seen in svp mutants, suggesting that OsMADS55 regulates flowering time associated with ambient temperature responses in Arabidopsis. Taken together, our data are consistent with functional conservation and diversification between Arabidopsis and rice SVP-like genes involved in controlling flowering time and flower development.",

keywords = "Flower development, Flowering time, OsMADS22, OsMADS55, Rice, SVP",

author = "Lee, {Jeong Hwan} and Park, {Soo Hyun} and Ahn, {Ji Hoon}",

note = "Funding Information: We thank Dr. Jeong Kook Kim for RNA materials and helpful discussion. Also, we thank H. Sommer for providing the 35S::SVP seeds used in this study. This work was supported by the Creative Research Initiatives of the National Research Foundation for the Ministry of Education, Science and Technology ( R16-2008-106–01000-0 to J.H.A.), by the Korea University Grant (to J.H.A. and J.H.L.), and by the Brain Korea 21 program (to J.H.L. and S.H.P.). ",

year = "2012",

month = apr,

doi = "10.1016/j.plantsci.2011.09.003",

language = "English",

volume = "185-186",

pages = "97--104",

journal = "Plant Science",

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AU - Lee, Jeong Hwan

AU - Park, Soo Hyun

AU - Ahn, Ji Hoon

N1 - Funding Information: We thank Dr. Jeong Kook Kim for RNA materials and helpful discussion. Also, we thank H. Sommer for providing the 35S::SVP seeds used in this study. This work was supported by the Creative Research Initiatives of the National Research Foundation for the Ministry of Education, Science and Technology ( R16-2008-106–01000-0 to J.H.A.), by the Korea University Grant (to J.H.A. and J.H.L.), and by the Brain Korea 21 program (to J.H.L. and S.H.P.).

PY - 2012/4

Y1 - 2012/4

N2 - MADS-box transcription factors play pivotal roles in several aspects of plant growth and development. The Arabidopsis SHORT VEGETATIVE PHASE (SVP) protein mediates the integration of signals involved in the control of flowering time and flower development by interacting with MADS-box proteins. In the rice genome, three SVP-like genes (OsMADS22, OsMADS47, and OsMADS55) are present. To investigate the functional conservation of these SVP-like genes in rice and Arabidopsis, the phenotypes of transgenic Arabidopsis plants overexpressing OsMADS22 and OsMADS55 were analyzed. Overexpression of OsMADS22 and OsMADS55 led to abnormal floral morphologies including leaf-like sepals, whereas only OsMADS55 expression caused delayed flowering via downregulation of FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1). Yeast two-hybrid assays revealed that OsMADS22 and OsMADS55 interacted with Arabidopsis AGL24 and AP1, but only OsMADS55 interacted with FLC. Overexpression of OsMADS55, but not OsMADS22, complemented the early flowering phenotype and ambient temperature-insensitive flowering phenotype seen in svp mutants, suggesting that OsMADS55 regulates flowering time associated with ambient temperature responses in Arabidopsis. Taken together, our data are consistent with functional conservation and diversification between Arabidopsis and rice SVP-like genes involved in controlling flowering time and flower development.

AB - MADS-box transcription factors play pivotal roles in several aspects of plant growth and development. The Arabidopsis SHORT VEGETATIVE PHASE (SVP) protein mediates the integration of signals involved in the control of flowering time and flower development by interacting with MADS-box proteins. In the rice genome, three SVP-like genes (OsMADS22, OsMADS47, and OsMADS55) are present. To investigate the functional conservation of these SVP-like genes in rice and Arabidopsis, the phenotypes of transgenic Arabidopsis plants overexpressing OsMADS22 and OsMADS55 were analyzed. Overexpression of OsMADS22 and OsMADS55 led to abnormal floral morphologies including leaf-like sepals, whereas only OsMADS55 expression caused delayed flowering via downregulation of FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1). Yeast two-hybrid assays revealed that OsMADS22 and OsMADS55 interacted with Arabidopsis AGL24 and AP1, but only OsMADS55 interacted with FLC. Overexpression of OsMADS55, but not OsMADS22, complemented the early flowering phenotype and ambient temperature-insensitive flowering phenotype seen in svp mutants, suggesting that OsMADS55 regulates flowering time associated with ambient temperature responses in Arabidopsis. Taken together, our data are consistent with functional conservation and diversification between Arabidopsis and rice SVP-like genes involved in controlling flowering time and flower development.

KW - Flower development

KW - Flowering time

KW - OsMADS22

KW - OsMADS55

KW - Rice

KW - SVP

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DO - 10.1016/j.plantsci.2011.09.003

M3 - Article

C2 - 22325870

AN - SCOPUS:84856668007

SN - 0168-9452

VL - 185-186

SP - 97

EP - 104

JO - Plant Science

JF - Plant Science

ER -

Functional conservation and diversification between rice OsMADS22/OsMADS55 and Arabidopsis SVP proteins

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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