Functional study of Capsicum annuum fatty acid desaturase 1 cDNA clone induced by Tobacco mosaic virus via microarray and virus-induced gene silencing

Ki Jeong Kim; Jee Hyuck Lim; Sanghyeob Lee; Young Jin Kim; Soo Bok Choi; Min Kyung Lee; Doil Choi; Kyung Hee Paek

doi:10.1016/j.bbrc.2007.07.095

Functional study of Capsicum annuum fatty acid desaturase 1 cDNA clone induced by Tobacco mosaic virus via microarray and virus-induced gene silencing

Ki Jeong Kim, Jee Hyuck Lim, Sanghyeob Lee, Young Jin Kim, Soo Bok Choi, Min Kyung Lee, Doil Choi, Kyung Hee Paek

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

A series of microarray analyses employing the expressed sequence tags (ESTs) of hot pepper was conducted in an effort to elucidate the molecular mechanisms inherent to hypersensitive response (HR) by viral or bacterial pathogens. There were 2535 ESTs exhibiting differential expression (over 2-fold changes) among about 5000 ESTs during viral or bacterial response. Further, via virus-induced gene silencing (VIGS) and TMV-infection studies, we were able to isolate several ESTs, which may be relevant to defense response against TMV. Of these ESTs, Capsicum annuum fatty acid desaturase 1 (CaFAD1) showed the distinct phenotype against TMV infection and thus was subjected to further study. CaFAD1-silenced plants showed weaker resistance against TMV-P₀ infection compared to TRV2 control plants. Also the suppression of FAD1 expression caused blocking of cell death induced by Bcl2-associated X (Bax) protein in tobacco plants. Therefore, this report presents that both microarray and VIGS approaches are feasible in hot pepper plants and the TMV-induced CaFAD1 plays a role in HR response.

Original language	English
Pages (from-to)	554-561
Number of pages	8
Journal	Biochemical and biophysical research communications
Volume	362
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2007.07.095
Publication status	Published - 2007 Oct 26

Bibliographical note

Funding Information:
This study was supported by the Korean BK21 program and a grant (R11-2003-008-02001-0) from the Korea Science and Engineering Foundation to the Plant Signaling Network Research Center (funding for K.-H. Paek).

Keywords

Capsicum annuum
Fatty acid desaturase
TMV
Virus-induced gene silencing

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2007.07.095

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Functional study of Capsicum annuum fatty acid desaturase 1 cDNA clone induced by Tobacco mosaic virus via microarray and virus-induced gene silencing. / Kim, Ki Jeong; Lim, Jee Hyuck; Lee, Sanghyeob et al.
In: Biochemical and biophysical research communications, Vol. 362, No. 3, 26.10.2007, p. 554-561.

Research output: Contribution to journal › Article › peer-review

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abstract = "A series of microarray analyses employing the expressed sequence tags (ESTs) of hot pepper was conducted in an effort to elucidate the molecular mechanisms inherent to hypersensitive response (HR) by viral or bacterial pathogens. There were 2535 ESTs exhibiting differential expression (over 2-fold changes) among about 5000 ESTs during viral or bacterial response. Further, via virus-induced gene silencing (VIGS) and TMV-infection studies, we were able to isolate several ESTs, which may be relevant to defense response against TMV. Of these ESTs, Capsicum annuum fatty acid desaturase 1 (CaFAD1) showed the distinct phenotype against TMV infection and thus was subjected to further study. CaFAD1-silenced plants showed weaker resistance against TMV-P0 infection compared to TRV2 control plants. Also the suppression of FAD1 expression caused blocking of cell death induced by Bcl2-associated X (Bax) protein in tobacco plants. Therefore, this report presents that both microarray and VIGS approaches are feasible in hot pepper plants and the TMV-induced CaFAD1 plays a role in HR response.",

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Functional study of Capsicum annuum fatty acid desaturase 1 cDNA clone induced by Tobacco mosaic virus via microarray and virus-induced gene silencing

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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