Functional study of hot pepper 26S proteasome subunit RPN7 induced by Tobacco mosaic virus from nuclear proteome analysis

Boo Ja Lee; Sun Jae Kwon; Sung Kyu Kim; Ki Jeong Kim; Chang Jin Park; Young Jin Kim; Ohkmae K. Park; Kyung Hee Paek

doi:10.1016/j.bbrc.2006.10.071

Functional study of hot pepper 26S proteasome subunit RPN7 induced by Tobacco mosaic virus from nuclear proteome analysis

Boo Ja Lee, Sun Jae Kwon, Sung Kyu Kim, Ki Jeong Kim, Chang Jin Park, Young Jin Kim, Ohkmae K. Park, Kyung Hee Paek

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

Two-dimensional gel electrophoresis (2-DE) was applied for the screening of Tobacco mosaic virus (TMV)-induced hot pepper (Capsicum annuum cv. Bugang) nuclear proteins. From differentially expressed protein spots, we acquired the matched peptide mass fingerprint (PMF) data, analyzed by MALDI-TOF MS, from the non-redundant hot pepper EST protein FASTA database using the VEMS 2.0 software. Among six identified nuclear proteins, the hot pepper 26S proteasome subunit RPN7 (CaRPN7) was subjected to further study. The level of CaRPN7 mRNA was specifically increased during incompatible TMV-P₀ interaction, but not during compatible TMV-P_1.2 interaction. When CaRPN7::GFP fusion protein was targeted in onion cells, the nuclei had been broken into pieces. In the hot pepper leaves, cell death was exacerbated and genomic DNA laddering was induced by Agrobacterium-mediated transient overexpression of CaPRN7. Thus, this report presents that the TMV-induced CaRPN7 may be involved in programmed cell death (PCD) in the hot pepper plant.

Original language	English
Pages (from-to)	405-411
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	351
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2006.10.071
Publication status	Published - 2006 Dec 15

Bibliographical note

Funding Information:
This study was supported by a Grant (R11-2003-008-02001-0) from the Korea Science and Engineering Foundation to the Plant Signaling Network Research Center. MALDI-TOF analysis was performed at the Korea Basic Science Institute, Seoul branch. We thank Dr. Bae, Jungdon and Mr. Kim, Dooil at KRIBB for communication of this study.

Keywords

CaRPN7
EST database
Hot pepper
Hypersensitive response
Nuclear protein
Programmed cell death
Proteasome
Proteomics
Subcellular localization
TMV

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2006.10.071

Cite this

@article{ed789284b1af4f57942792420ed38469,

title = "Functional study of hot pepper 26S proteasome subunit RPN7 induced by Tobacco mosaic virus from nuclear proteome analysis",

abstract = "Two-dimensional gel electrophoresis (2-DE) was applied for the screening of Tobacco mosaic virus (TMV)-induced hot pepper (Capsicum annuum cv. Bugang) nuclear proteins. From differentially expressed protein spots, we acquired the matched peptide mass fingerprint (PMF) data, analyzed by MALDI-TOF MS, from the non-redundant hot pepper EST protein FASTA database using the VEMS 2.0 software. Among six identified nuclear proteins, the hot pepper 26S proteasome subunit RPN7 (CaRPN7) was subjected to further study. The level of CaRPN7 mRNA was specifically increased during incompatible TMV-P0 interaction, but not during compatible TMV-P1.2 interaction. When CaRPN7::GFP fusion protein was targeted in onion cells, the nuclei had been broken into pieces. In the hot pepper leaves, cell death was exacerbated and genomic DNA laddering was induced by Agrobacterium-mediated transient overexpression of CaPRN7. Thus, this report presents that the TMV-induced CaRPN7 may be involved in programmed cell death (PCD) in the hot pepper plant.",

keywords = "CaRPN7, EST database, Hot pepper, Hypersensitive response, Nuclear protein, Programmed cell death, Proteasome, Proteomics, Subcellular localization, TMV",

author = "Lee, {Boo Ja} and Kwon, {Sun Jae} and Kim, {Sung Kyu} and Kim, {Ki Jeong} and Park, {Chang Jin} and Kim, {Young Jin} and Park, {Ohkmae K.} and Paek, {Kyung Hee}",

note = "Funding Information: This study was supported by a Grant (R11-2003-008-02001-0) from the Korea Science and Engineering Foundation to the Plant Signaling Network Research Center. MALDI-TOF analysis was performed at the Korea Basic Science Institute, Seoul branch. We thank Dr. Bae, Jungdon and Mr. Kim, Dooil at KRIBB for communication of this study. ",

year = "2006",

month = dec,

day = "15",

doi = "10.1016/j.bbrc.2006.10.071",

language = "English",

volume = "351",

pages = "405--411",

journal = "Biochemical and biophysical research communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "2",

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TY - JOUR

T1 - Functional study of hot pepper 26S proteasome subunit RPN7 induced by Tobacco mosaic virus from nuclear proteome analysis

AU - Lee, Boo Ja

AU - Kwon, Sun Jae

AU - Kim, Sung Kyu

AU - Kim, Ki Jeong

AU - Park, Chang Jin

AU - Kim, Young Jin

AU - Park, Ohkmae K.

AU - Paek, Kyung Hee

N1 - Funding Information: This study was supported by a Grant (R11-2003-008-02001-0) from the Korea Science and Engineering Foundation to the Plant Signaling Network Research Center. MALDI-TOF analysis was performed at the Korea Basic Science Institute, Seoul branch. We thank Dr. Bae, Jungdon and Mr. Kim, Dooil at KRIBB for communication of this study.

PY - 2006/12/15

Y1 - 2006/12/15

N2 - Two-dimensional gel electrophoresis (2-DE) was applied for the screening of Tobacco mosaic virus (TMV)-induced hot pepper (Capsicum annuum cv. Bugang) nuclear proteins. From differentially expressed protein spots, we acquired the matched peptide mass fingerprint (PMF) data, analyzed by MALDI-TOF MS, from the non-redundant hot pepper EST protein FASTA database using the VEMS 2.0 software. Among six identified nuclear proteins, the hot pepper 26S proteasome subunit RPN7 (CaRPN7) was subjected to further study. The level of CaRPN7 mRNA was specifically increased during incompatible TMV-P0 interaction, but not during compatible TMV-P1.2 interaction. When CaRPN7::GFP fusion protein was targeted in onion cells, the nuclei had been broken into pieces. In the hot pepper leaves, cell death was exacerbated and genomic DNA laddering was induced by Agrobacterium-mediated transient overexpression of CaPRN7. Thus, this report presents that the TMV-induced CaRPN7 may be involved in programmed cell death (PCD) in the hot pepper plant.

AB - Two-dimensional gel electrophoresis (2-DE) was applied for the screening of Tobacco mosaic virus (TMV)-induced hot pepper (Capsicum annuum cv. Bugang) nuclear proteins. From differentially expressed protein spots, we acquired the matched peptide mass fingerprint (PMF) data, analyzed by MALDI-TOF MS, from the non-redundant hot pepper EST protein FASTA database using the VEMS 2.0 software. Among six identified nuclear proteins, the hot pepper 26S proteasome subunit RPN7 (CaRPN7) was subjected to further study. The level of CaRPN7 mRNA was specifically increased during incompatible TMV-P0 interaction, but not during compatible TMV-P1.2 interaction. When CaRPN7::GFP fusion protein was targeted in onion cells, the nuclei had been broken into pieces. In the hot pepper leaves, cell death was exacerbated and genomic DNA laddering was induced by Agrobacterium-mediated transient overexpression of CaPRN7. Thus, this report presents that the TMV-induced CaRPN7 may be involved in programmed cell death (PCD) in the hot pepper plant.

KW - CaRPN7

KW - EST database

KW - Hot pepper

KW - Hypersensitive response

KW - Nuclear protein

KW - Programmed cell death

KW - Proteasome

KW - Proteomics

KW - Subcellular localization

KW - TMV

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SN - 0006-291X

VL - 351

SP - 405

EP - 411

JO - Biochemical and biophysical research communications

JF - Biochemical and biophysical research communications

IS - 2

ER -

Functional study of hot pepper 26S proteasome subunit RPN7 induced by Tobacco mosaic virus from nuclear proteome analysis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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