Fungal pretreatment of lignocellulose by Phanerochaete chrysosporium to produce ethanol from rice straw

Jin Seop Bak, Ja Kyong Ko, In Geol Choi, Yong Cheol Park, Jin Ho Seo, Kyoung Heon Kim

Research output: Contribution to journalArticlepeer-review

171 Citations (Scopus)


Phanerochaete chrysosporium is a wood-rot fungus that is capable of degrading lignin via its lignolytic system. In this study, an environmentally friendly fungal pretreatment process that produces less inhibitory substances than conventional methods was developed using P. chrysosporium and then evaluated by various analytical methods. To maximize the production of manganese peroxidase, which is the primary lignin-degrading enzyme, culture medium was optimized using response surface methodologies including the Plackett-Burman design and the Box-Behnken design. Fermentation of 100 g of rice straw feedstock containing 35.7 g of glucan (mainly in the form of cellulose) by cultivation with P. chrysosporium for 15 days in the media optimized by response surface methodology was resulted in a yield of 29.0 g of glucan that had an enzymatic digestibility of 64.9% of the theoretical maximum glucose yield. In addition, scanning electronic microscopy, confocal laser scanning microscopy, and X-ray diffractometry revealed significant microstructural changes, fungal growth, and a reduction of the crystallinity index in the pretreated rice straw, respectively. When the fungal-pretreated rice straw was used as a substrate for ethanol production in simultaneous saccharification and fermentation (SSF) for 24 h, the ethanol concentration, production yield and the productivity were 9.49 g/L, 58.2% of the theoretical maximum, and 0.40 g/L/h, respectively. Based on these experimental data, if 100 g of rice straw are subjected to fungal pretreatment and SSF, 9.9 g of ethanol can be produced after 96 h, which is 62.7% of the theoretical maximum ethanol yield.

Original languageEnglish
Pages (from-to)471-482
Number of pages12
JournalBiotechnology and Bioengineering
Issue number3
Publication statusPublished - 2009 Oct 15


  • Ethanol
  • Fungal pretreatment
  • Glyoxal oxidase
  • Lignocellulose
  • Manganese peroxidase
  • Phanerochaete chrysosporium

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


Dive into the research topics of 'Fungal pretreatment of lignocellulose by Phanerochaete chrysosporium to produce ethanol from rice straw'. Together they form a unique fingerprint.

Cite this