GABAA receptor-associated protein (GABARAP) is a 14-kDa cytoplasmic protein initially identified as a molecular chaperone for GABA A receptor in cortical neurons. However, evidence indicates that the function of GABARAP is much broader than a specific role in neuronal cells. As an initial step to define the biological role of GABARAP, we searched for binding partners of GABARAP using co-immunoprecipitation coupled with LC-MS/MS analysis. As a result, DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47), recently identified as RNA helicase, is identified as a binding partner of GABARAP. An interaction between GABARAP and DDX 47 was further confirmed by yeast two-hybrid systems. Further, co-transfection of GABARAP and DDX47 cDNA into a tumor cell line induces apoptosis. This result may be the first evidence showing that GABARAP and DDX 47 are involved in the apoptotic process.
Bibliographical noteFunding Information:
This work was supported by the research fund of Hanyang University (HY-2004-S).
- DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47)
- GABA receptor-associated protein (GABARAP)
- RNA helicase
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology