GC-TOF/MS-Based Metabolomics for Comparison of Volar and Non-Volar Skin Types

Ting Bu, Ming Zhang, Sun Hee Lee, Yu Eun Cheong, Yukyung Park, Kyoung Heon Kim, Dongwon Kim, Sooah Kim

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Skin has heterogenous identities on different body sites despite similar cellular compositions. There are two types of skin, volar (palmoplantar) and non-volar (dorsal), which are characterized by epidermal thickness, pigmentation, and presence of hair follicles. However, the mechanisms underlying the development of these different skin types remain unclear. To investigate these, we profiled the cellular metabolites of volar and non-volar skin in mice using gas chromatography-time-of-flight/mass spectrometry (GC-TOF/MS), and further assessed the metabolic differences between them. In total, 96 metabolites from both volar and non-volar skin of mice were identified using the BinBase database system. Metabolomics analysis revealed important differences associated with amino acid metabolism (phenylalanine, tyrosine, and tryptophan biosynthesis; aspartate and glutamate metabolism), sugar metabolism (pentose phosphate pathway), and nucleotide metabolism (pyrimidine metabolism) in volar skin. Fifty metabolites were identified as potential biomarkers differentiating the physiological characteristics of these skin types. Of these, nine were highly increased whereas 41 were significantly decreased in volar skin compared with those in non-volar skin. Overall, these results provide valuable information for understanding the metabolic differences between volar and non-volar skin.

Original languageEnglish
Article number717
Issue number8
Publication statusPublished - 2022 Aug

Bibliographical note

Funding Information:
This study was financially supported by the Young Researcher Program (number 2020R1G1A100826811) from the National Research Foundation of Korea (NRF) to S.K. Additionally, this work was supported by the National Research Foundation of Korea grant funded by the Korean government (Ministry of Science and ICT) (number 2020R1C1C1005206) to D.K.

Publisher Copyright:
© 2022 by the authors.


  • metabolomic analysis
  • non-volar skin
  • volar skin

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology


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