GDSL-lipase1 (CaGL1) contributes to wound stress resistance by modulation of CaPR-4 expression in hot pepper

Ki Jeong Kim; Jee Hyuck Lim; Min Jung Kim; Taesung Kim; Hyen Mi Chung; Kyung Hee Paek

doi:10.1016/j.bbrc.2008.07.120

GDSL-lipase1 (CaGL1) contributes to wound stress resistance by modulation of CaPR-4 expression in hot pepper

Ki Jeong Kim, Jee Hyuck Lim, Min Jung Kim, Taesung Kim, Hyen Mi Chung, Kyung Hee Paek

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

A full length cDNA clone encoding Capsicum annuum GDSL-lipase 1 (CaGL1) was isolated by microarray analysis. The expression of CaGL1 was triggered by methyl jasmonic acid (MeJA), an important signal in abiotic/biotic stress response. However, the expression of this gene was not increased by the application of salicylic acid (SA) or ethylene treatment. And, local/systemic wounding stimuli resulted in rapid accumulation of CaGL1 mRNA. However, CaGL1 was not specifically induced during the hypersensitive response upon Tobacco mosaic virus (TMV) inoculation. By using a virus-induced gene silencing (VIGS)-based reverse genetic approach, it was observed that the suppression of CaGL1 attenuates the expression of Capsicum annuum pathogenesis-related protein 4 (CaPR-4) during wound stress. However, the CaPR-4 transcript level induced by TMV was not regulated by CaGL1 expression. These results indicate that CaGL1 may be involved in signaling pathway of MeJA and/or the wound responses through CaPR-4 expression modulation.

Original language	English
Pages (from-to)	693-698
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	374
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2008.07.120
Publication status	Published - 2008 Oct 3

Keywords

CaGL1
CaPR-4
Hot pepper (Capsicum annuum L.)
MeJA
TMV
Virus-induced gene silencing
Wound

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2008.07.120

Cite this

@article{a5611513cad944e291162e516d040e75,

title = "GDSL-lipase1 (CaGL1) contributes to wound stress resistance by modulation of CaPR-4 expression in hot pepper",

abstract = "A full length cDNA clone encoding Capsicum annuum GDSL-lipase 1 (CaGL1) was isolated by microarray analysis. The expression of CaGL1 was triggered by methyl jasmonic acid (MeJA), an important signal in abiotic/biotic stress response. However, the expression of this gene was not increased by the application of salicylic acid (SA) or ethylene treatment. And, local/systemic wounding stimuli resulted in rapid accumulation of CaGL1 mRNA. However, CaGL1 was not specifically induced during the hypersensitive response upon Tobacco mosaic virus (TMV) inoculation. By using a virus-induced gene silencing (VIGS)-based reverse genetic approach, it was observed that the suppression of CaGL1 attenuates the expression of Capsicum annuum pathogenesis-related protein 4 (CaPR-4) during wound stress. However, the CaPR-4 transcript level induced by TMV was not regulated by CaGL1 expression. These results indicate that CaGL1 may be involved in signaling pathway of MeJA and/or the wound responses through CaPR-4 expression modulation.",

keywords = "CaGL1, CaPR-4, Hot pepper (Capsicum annuum L.), MeJA, TMV, Virus-induced gene silencing, Wound",

author = "Kim, {Ki Jeong} and Lim, {Jee Hyuck} and Kim, {Min Jung} and Taesung Kim and Chung, {Hyen Mi} and Paek, {Kyung Hee}",

note = "Funding Information: This study was supported by a Grant (R11-2003-008-02001-0) from the Korea Science and Engineering Foundation to the Plant Signaling Network Research Center. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2008",

month = oct,

day = "3",

doi = "10.1016/j.bbrc.2008.07.120",

language = "English",

volume = "374",

pages = "693--698",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "4",

}

TY - JOUR

T1 - GDSL-lipase1 (CaGL1) contributes to wound stress resistance by modulation of CaPR-4 expression in hot pepper

AU - Kim, Ki Jeong

AU - Lim, Jee Hyuck

AU - Kim, Min Jung

AU - Kim, Taesung

AU - Chung, Hyen Mi

AU - Paek, Kyung Hee

N1 - Funding Information: This study was supported by a Grant (R11-2003-008-02001-0) from the Korea Science and Engineering Foundation to the Plant Signaling Network Research Center. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2008/10/3

Y1 - 2008/10/3

N2 - A full length cDNA clone encoding Capsicum annuum GDSL-lipase 1 (CaGL1) was isolated by microarray analysis. The expression of CaGL1 was triggered by methyl jasmonic acid (MeJA), an important signal in abiotic/biotic stress response. However, the expression of this gene was not increased by the application of salicylic acid (SA) or ethylene treatment. And, local/systemic wounding stimuli resulted in rapid accumulation of CaGL1 mRNA. However, CaGL1 was not specifically induced during the hypersensitive response upon Tobacco mosaic virus (TMV) inoculation. By using a virus-induced gene silencing (VIGS)-based reverse genetic approach, it was observed that the suppression of CaGL1 attenuates the expression of Capsicum annuum pathogenesis-related protein 4 (CaPR-4) during wound stress. However, the CaPR-4 transcript level induced by TMV was not regulated by CaGL1 expression. These results indicate that CaGL1 may be involved in signaling pathway of MeJA and/or the wound responses through CaPR-4 expression modulation.

AB - A full length cDNA clone encoding Capsicum annuum GDSL-lipase 1 (CaGL1) was isolated by microarray analysis. The expression of CaGL1 was triggered by methyl jasmonic acid (MeJA), an important signal in abiotic/biotic stress response. However, the expression of this gene was not increased by the application of salicylic acid (SA) or ethylene treatment. And, local/systemic wounding stimuli resulted in rapid accumulation of CaGL1 mRNA. However, CaGL1 was not specifically induced during the hypersensitive response upon Tobacco mosaic virus (TMV) inoculation. By using a virus-induced gene silencing (VIGS)-based reverse genetic approach, it was observed that the suppression of CaGL1 attenuates the expression of Capsicum annuum pathogenesis-related protein 4 (CaPR-4) during wound stress. However, the CaPR-4 transcript level induced by TMV was not regulated by CaGL1 expression. These results indicate that CaGL1 may be involved in signaling pathway of MeJA and/or the wound responses through CaPR-4 expression modulation.

KW - CaGL1

KW - CaPR-4

KW - Hot pepper (Capsicum annuum L.)

KW - MeJA

KW - TMV

KW - Virus-induced gene silencing

KW - Wound

UR - http://www.scopus.com/inward/record.url?scp=49649089052&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49649089052&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2008.07.120

DO - 10.1016/j.bbrc.2008.07.120

M3 - Article

C2 - 18680725

AN - SCOPUS:49649089052

SN - 0006-291X

VL - 374

SP - 693

EP - 698

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

GDSL-lipase1 (CaGL1) contributes to wound stress resistance by modulation of CaPR-4 expression in hot pepper

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this