GDSL-lipase1 (CaGL1) contributes to wound stress resistance by modulation of CaPR-4 expression in hot pepper

Ki Jeong Kim, Jee Hyuck Lim, Min Jung Kim, Taesung Kim, Hyen Mi Chung, Kyung Hee Paek

    Research output: Contribution to journalArticlepeer-review

    43 Citations (Scopus)

    Abstract

    A full length cDNA clone encoding Capsicum annuum GDSL-lipase 1 (CaGL1) was isolated by microarray analysis. The expression of CaGL1 was triggered by methyl jasmonic acid (MeJA), an important signal in abiotic/biotic stress response. However, the expression of this gene was not increased by the application of salicylic acid (SA) or ethylene treatment. And, local/systemic wounding stimuli resulted in rapid accumulation of CaGL1 mRNA. However, CaGL1 was not specifically induced during the hypersensitive response upon Tobacco mosaic virus (TMV) inoculation. By using a virus-induced gene silencing (VIGS)-based reverse genetic approach, it was observed that the suppression of CaGL1 attenuates the expression of Capsicum annuum pathogenesis-related protein 4 (CaPR-4) during wound stress. However, the CaPR-4 transcript level induced by TMV was not regulated by CaGL1 expression. These results indicate that CaGL1 may be involved in signaling pathway of MeJA and/or the wound responses through CaPR-4 expression modulation.

    Original languageEnglish
    Pages (from-to)693-698
    Number of pages6
    JournalBiochemical and biophysical research communications
    Volume374
    Issue number4
    DOIs
    Publication statusPublished - 2008 Oct 3

    Bibliographical note

    Funding Information:
    This study was supported by a Grant (R11-2003-008-02001-0) from the Korea Science and Engineering Foundation to the Plant Signaling Network Research Center.

    Copyright:
    Copyright 2008 Elsevier B.V., All rights reserved.

    Keywords

    • CaGL1
    • CaPR-4
    • Hot pepper (Capsicum annuum L.)
    • MeJA
    • TMV
    • Virus-induced gene silencing
    • Wound

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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