Abstract
A full length cDNA clone encoding Capsicum annuum GDSL-lipase 1 (CaGL1) was isolated by microarray analysis. The expression of CaGL1 was triggered by methyl jasmonic acid (MeJA), an important signal in abiotic/biotic stress response. However, the expression of this gene was not increased by the application of salicylic acid (SA) or ethylene treatment. And, local/systemic wounding stimuli resulted in rapid accumulation of CaGL1 mRNA. However, CaGL1 was not specifically induced during the hypersensitive response upon Tobacco mosaic virus (TMV) inoculation. By using a virus-induced gene silencing (VIGS)-based reverse genetic approach, it was observed that the suppression of CaGL1 attenuates the expression of Capsicum annuum pathogenesis-related protein 4 (CaPR-4) during wound stress. However, the CaPR-4 transcript level induced by TMV was not regulated by CaGL1 expression. These results indicate that CaGL1 may be involved in signaling pathway of MeJA and/or the wound responses through CaPR-4 expression modulation.
Original language | English |
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Pages (from-to) | 693-698 |
Number of pages | 6 |
Journal | Biochemical and biophysical research communications |
Volume | 374 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2008 Oct 3 |
Keywords
- CaGL1
- CaPR-4
- Hot pepper (Capsicum annuum L.)
- MeJA
- TMV
- Virus-induced gene silencing
- Wound
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology