GDSL-lipase1 (CaGL1) contributes to wound stress resistance by modulation of CaPR-4 expression in hot pepper

Ki Jeong Kim, Jee Hyuck Lim, Min Jung Kim, Taesung Kim, Hyen Mi Chung, Kyung Hee Paek

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

A full length cDNA clone encoding Capsicum annuum GDSL-lipase 1 (CaGL1) was isolated by microarray analysis. The expression of CaGL1 was triggered by methyl jasmonic acid (MeJA), an important signal in abiotic/biotic stress response. However, the expression of this gene was not increased by the application of salicylic acid (SA) or ethylene treatment. And, local/systemic wounding stimuli resulted in rapid accumulation of CaGL1 mRNA. However, CaGL1 was not specifically induced during the hypersensitive response upon Tobacco mosaic virus (TMV) inoculation. By using a virus-induced gene silencing (VIGS)-based reverse genetic approach, it was observed that the suppression of CaGL1 attenuates the expression of Capsicum annuum pathogenesis-related protein 4 (CaPR-4) during wound stress. However, the CaPR-4 transcript level induced by TMV was not regulated by CaGL1 expression. These results indicate that CaGL1 may be involved in signaling pathway of MeJA and/or the wound responses through CaPR-4 expression modulation.

Original languageEnglish
Pages (from-to)693-698
Number of pages6
JournalBiochemical and biophysical research communications
Volume374
Issue number4
DOIs
Publication statusPublished - 2008 Oct 3

Keywords

  • CaGL1
  • CaPR-4
  • Hot pepper (Capsicum annuum L.)
  • MeJA
  • TMV
  • Virus-induced gene silencing
  • Wound

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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