TY - JOUR
T1 - Gemmobacter aquarius sp. Nov., runella rosea sp. nov. and flavobacterium fluviale sp. nov., isolated from the namhangang river system
AU - Baek, Min Gyung
AU - Shin, Su Kyoung
AU - Yi, Hana
N1 - Funding Information:
This study was supported by the research grant ‘Survey of Fresh-water Organisms and Specimen Collection (Prokaryotes)’ from the Nakdonggang National Institute of Biological Resources of the Ministry of Environment in Korea and by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIT) (No. 2019R1F1A1059925).
Funding Information:
This study was supported by the research grant ?Survey of Freshwater Organisms and Specimen Collection (Prokaryotes)? from the Nakdonggang National Institute of Biological Resources of the Ministry of Environment in Korea and by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIT) (No. 2019R1F1A1059925).
Publisher Copyright:
© 2020 The Authors.
PY - 2020
Y1 - 2020
N2 - Three bacterial strains, namely HYN0069T, HYN0085T and HYN0086T, were isolated from freshwater samples taken from the Namhangan River system in Korea. 16S rRNA gene sequence similarities and phylogenetic tree topologies indicated that the three strains belonged to the genera Gemmobacter, Runella and Flavobacterium by showing the highest sequence similarities with Gemmobacter straminiformis (98.4%), Runella aurantiaca (98.3%) and Flavobacterium chungangense (98.1%). No bacterial species with validly published names showed 98.7% or higher sequence similarity with the novel isolates. The average nucleotide identities between the genome sequences of the three new isolates and the three closest neighbours were 80.2–92.0%, all below the threshold for bacterial species delineation (95–96%). Many biochemical and physiological features also discriminated the isolates from previously known species of the genera Gemmobacter, Runella and Flavobacterium. Based on the phylogenetic and phenotypic data presented in this study, we suggest three novel species with the following names: Gemmobacter aquarius sp. nov. (type strain HYN0069T=KACC 19488T=NBRC 113115T), Runella rosea sp. nov. (type strain HYN0085T=KACC 19490T=NBRC 113116T) and Flavobacterium fluviale sp. nov. (type strain HYN0086T=KACC 19489T=NBRC 113117T).
AB - Three bacterial strains, namely HYN0069T, HYN0085T and HYN0086T, were isolated from freshwater samples taken from the Namhangan River system in Korea. 16S rRNA gene sequence similarities and phylogenetic tree topologies indicated that the three strains belonged to the genera Gemmobacter, Runella and Flavobacterium by showing the highest sequence similarities with Gemmobacter straminiformis (98.4%), Runella aurantiaca (98.3%) and Flavobacterium chungangense (98.1%). No bacterial species with validly published names showed 98.7% or higher sequence similarity with the novel isolates. The average nucleotide identities between the genome sequences of the three new isolates and the three closest neighbours were 80.2–92.0%, all below the threshold for bacterial species delineation (95–96%). Many biochemical and physiological features also discriminated the isolates from previously known species of the genera Gemmobacter, Runella and Flavobacterium. Based on the phylogenetic and phenotypic data presented in this study, we suggest three novel species with the following names: Gemmobacter aquarius sp. nov. (type strain HYN0069T=KACC 19488T=NBRC 113115T), Runella rosea sp. nov. (type strain HYN0085T=KACC 19490T=NBRC 113116T) and Flavobacterium fluviale sp. nov. (type strain HYN0086T=KACC 19489T=NBRC 113117T).
KW - Flavobacterium
KW - Flavobacterium fluviale
KW - Gemmobacter
KW - Gemmobacter aquarius
KW - Runella
KW - Runella rosea
UR - http://www.scopus.com/inward/record.url?scp=85096938449&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.004455
DO - 10.1099/ijsem.0.004455
M3 - Article
C2 - 32936754
AN - SCOPUS:85096938449
SN - 1466-5026
VL - 70
SP - 5640
EP - 5647
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 11
M1 - 004455
ER -