Gemmobacter lutimaris sp. nov., a marine bacterium isolated from a tidal flat

A novel cream-pigmented marine bacterium, designated strain YJ-T1-11^T, was isolated from a tidal flat at Yeongjong-do, Republic of Korea. Cells were rod-shaped, non-motile, aerobic, Gram-reaction-negative, oxidase-positive and catalasepositive. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain YJ-T1-11^T clustered with Gemmobacter fontiphilus JS43^T (98.3%) within the genus Gemmobacter and its closest neighbours were G.emmobacter aquatilis DSM 3857^T (98.5%), Gemmobacter aquaticus A1-9^T (98.4%), Gemmobacter lanyuensis Orc-4^T (98.4%), Gemmobacter fontiphilus JS43^T (98.3%), Gemmobacter caeni DCA-1^T (98.2%), Gemmobacter nanjingensis Y12^T (97.5%) and Gemmobacter tilapiae Ruye-53^T (97.2%). Average nucleotide identity values between the genome sequences of strain YJ-T1-11^T and the related type strains ranged from 77.08 to 90.48%. The predominant fatty acid of strain YJ-T1-11^T was summed feature 8 (comprising C_{18: 1}ω7c and/or C_{18: 1}ω6c). The major isoprenoid quinone was Q-10 and the DNA G+C content was 65.6 mol%. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol and three unidentified lipids. The DNA–DNA relatedness values between strain YJ-T1-11^T and the type strains of the 12 phylogenetically related species of the genus Gemmobacter were 23.6–53.7%. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain YJ-T1-11^T is considered to represent a novel species of the genus Gemmobacter, for which the name Gemmobacter lutimaris sp. nov. is proposed. The type strain is YJ-T1-11^T (=KCTC 62715^T =JCM 32828^T).