Gene expression analysis and classification of mode of toxicity of polycyclic aromatic hydrocarbons (PAHs) in Escherichia coli

Yeon Seok Kim, Jiho Min, Han Na Hong, Ji Hyun Park, Kyeong Seo Park, Man Bock Gu

    Research output: Contribution to journalArticlepeer-review

    27 Citations (Scopus)

    Abstract

    Escherichia coli is known to respond to certain toxic chemicals through an increased expression of various stress genes. In this study, therefore, the expression of recA, katG, fabA and grpE genes was used as a representative for DNA, oxidative, membrane and protein damage, respectively, after E. coli was exposed to different polycyclic aromatic hydrocarbons (PAHs), i.e., phenanthrene, naphthalene and benzo[a]pyrene. To accomplish this, the expression levels of these four genes were quantified using a real-time RT-PCR analysis when E. coli cultures were under stressful conditions, such as those caused by an exposure to mitomycin C, hydrogen peroxide and phenol. It was found that the primary toxic effect of each chemical is clearly seen when the expression levels of the different genes are compared. Tests with the PAHs showed naphthalene and benzo[a]pyrene to be genotoxic, while phenanthrene had no clear effect on the expression of any of these genes. Based on these results, the effects due to these toxic chemicals and the extent of each stress can be evaluated with ease using the expression levels of different stress responsive genes.

    Original languageEnglish
    Pages (from-to)1243-1248
    Number of pages6
    JournalChemosphere
    Volume66
    Issue number7
    DOIs
    Publication statusPublished - 2007 Jan

    Bibliographical note

    Funding Information:
    This work was supported by the Korea University Grant.

    Copyright:
    Copyright 2020 Elsevier B.V., All rights reserved.

    Keywords

    • Gene expression analysis
    • Polycyclic aromatic hydrocarbons (PAHs)
    • Real-time RT-PCR
    • Stress specific responsive gene

    ASJC Scopus subject areas

    • Environmental Engineering
    • Environmental Chemistry
    • General Chemistry
    • Pollution
    • Health, Toxicology and Mutagenesis

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