TY - JOUR
T1 - Gene expression analysis and classification of mode of toxicity of polycyclic aromatic hydrocarbons (PAHs) in Escherichia coli
AU - Kim, Yeon Seok
AU - Min, Jiho
AU - Hong, Han Na
AU - Park, Ji Hyun
AU - Park, Kyeong Seo
AU - Gu, Man Bock
N1 - Funding Information:
This work was supported by the Korea University Grant.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2007/1
Y1 - 2007/1
N2 - Escherichia coli is known to respond to certain toxic chemicals through an increased expression of various stress genes. In this study, therefore, the expression of recA, katG, fabA and grpE genes was used as a representative for DNA, oxidative, membrane and protein damage, respectively, after E. coli was exposed to different polycyclic aromatic hydrocarbons (PAHs), i.e., phenanthrene, naphthalene and benzo[a]pyrene. To accomplish this, the expression levels of these four genes were quantified using a real-time RT-PCR analysis when E. coli cultures were under stressful conditions, such as those caused by an exposure to mitomycin C, hydrogen peroxide and phenol. It was found that the primary toxic effect of each chemical is clearly seen when the expression levels of the different genes are compared. Tests with the PAHs showed naphthalene and benzo[a]pyrene to be genotoxic, while phenanthrene had no clear effect on the expression of any of these genes. Based on these results, the effects due to these toxic chemicals and the extent of each stress can be evaluated with ease using the expression levels of different stress responsive genes.
AB - Escherichia coli is known to respond to certain toxic chemicals through an increased expression of various stress genes. In this study, therefore, the expression of recA, katG, fabA and grpE genes was used as a representative for DNA, oxidative, membrane and protein damage, respectively, after E. coli was exposed to different polycyclic aromatic hydrocarbons (PAHs), i.e., phenanthrene, naphthalene and benzo[a]pyrene. To accomplish this, the expression levels of these four genes were quantified using a real-time RT-PCR analysis when E. coli cultures were under stressful conditions, such as those caused by an exposure to mitomycin C, hydrogen peroxide and phenol. It was found that the primary toxic effect of each chemical is clearly seen when the expression levels of the different genes are compared. Tests with the PAHs showed naphthalene and benzo[a]pyrene to be genotoxic, while phenanthrene had no clear effect on the expression of any of these genes. Based on these results, the effects due to these toxic chemicals and the extent of each stress can be evaluated with ease using the expression levels of different stress responsive genes.
KW - Gene expression analysis
KW - Polycyclic aromatic hydrocarbons (PAHs)
KW - Real-time RT-PCR
KW - Stress specific responsive gene
UR - http://www.scopus.com/inward/record.url?scp=33845198735&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845198735&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2006.07.040
DO - 10.1016/j.chemosphere.2006.07.040
M3 - Article
C2 - 16949636
AN - SCOPUS:33845198735
SN - 0045-6535
VL - 66
SP - 1243
EP - 1248
JO - Chemosphere
JF - Chemosphere
IS - 7
ER -