Generation and analysis of a complete mutant set for the Arabidopsis FT/TFL1 family shows specific effects on thermo-sensitive flowering regulation

Wanhui Kim, Tae Im Park, Seong Jeon Yoo, A. Rim Jun, Ji Hoon Ahn

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

The FLOWERING LOCUS T (FT)/TERMINAL FLOWER 1 (TFL1) family proteins play an important role in the regulation of flowering time. In the Arabidopsis thaliana genome, there are six genes in the FT/TFL1 family. To determine how these FT/TFL1 family genes contribute to the regulation of flowering time, this study generated a comprehensive set of mutants (sixty-three multiple mutants in all combinations) of the FT/TFL1 family genes and analysed their flowering times at 23 and 16°C under long-day conditions. The analysis confirmed that FT and TFL1 are major determinants of flowering time under long-day conditions. At 23 °C, ft-10 tsf-1 mft-2 showed the latest flowering, whereas tfl1-20 atc-2 bft-2 showed the earliest flowering. Flowering occurred in the sextuple mutants. Introduction of tsf-1 led to reduced sensitivity to ambient temperature change. Introduction of tfl1-20 caused a stronger effect in accelerating flowering time at 16 °C than at 23 °C. Overexpression of miR156 did not block flowering of sextuple mutants, suggesting that there is a pathway to induce flowering independent of the FT/TFL1 pathway and miR156 pathway. This study proposes that this mutant population will be useful in further investigation of the functions of the FT/TFL1 family genes in plant development.

Original languageEnglish
Pages (from-to)1715-1729
Number of pages15
JournalJournal of experimental botany
Volume64
Issue number6
DOIs
Publication statusPublished - 2013 Apr

Bibliographical note

Funding Information:
This work was supported by the Creative Research Initiatives of the National Research Foundation for the Ministry of Education, Science and Technology (R16-2008-106-01000-0 to JHA), the Korea University (WK), and the BK 21 Program (to WK, TIP, SJY, and ARJ). The authors thank SY Yoo for her initial support and MH Lee for his technical assistance.

Keywords

  • ATC
  • Arabidopsis thaliana
  • BFT
  • FT
  • MFT
  • TFL1
  • TSF.
  • flowering time
  • miR156

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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