Generation and evaluation of the efficacy of rhesus monkey soluble cytotoxic T lymphocyte-associated antigen-4 in the allogeneic mixed lymphocyte reaction

Jung Sik Kim; Inho Choi; Han Hyoung Lee; Suk Jun Lee; Manheum Na; Sang Hoon Kim; Jihye Han; Joonbeom Bae; Sang Pil Choi; Sang Joon Kim; Chung Gyu Park; Taehoon Chun

doi:10.1007/s10529-012-1026-0

Generation and evaluation of the efficacy of rhesus monkey soluble cytotoxic T lymphocyte-associated antigen-4 in the allogeneic mixed lymphocyte reaction

Jung Sik Kim, Inho Choi, Han Hyoung Lee, Suk Jun Lee, Manheum Na, Sang Hoon Kim, Jihye Han, Joonbeom Bae, Sang Pil Choi, Sang Joon Kim, Chung Gyu Park, Taehoon Chun

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4; CD152) is a transmembrane protein that is structurally similar to CD28. As CTLA-4 has a much higher binding affinity to B7 than CD28, several approaches using soluble CTLA-4 have been tried to down-regulate T cell activity by blocking the interaction between CD28 and B7. We constructed soluble rhesus monkey CTLA-4 immunoglobulin (CTLA-4Ig) containing a critical binding site to B7 combined with a constant Ig heavy chain region in a mammalian system. Flow cytometry analyses indicated that soluble rhesus monkey CTLA-4Ig bound to rhesus monkey CD86 (B7. 2). Moreover, soluble rhesus monkey CTLA-4Ig more effectively blocked the rhesus monkey-rhesus monkey allogeneic mixed lymphocyte reaction compared with that of humans. These results indicate that soluble rhesus monkey CTLA-4Ig may be useful in preclinical trials in a rhesus monkey model.

Original language	English
Pages (from-to)	2191-2197
Number of pages	7
Journal	Biotechnology letters
Volume	34
Issue number	12
DOIs	https://doi.org/10.1007/s10529-012-1026-0
Publication status	Published - 2012 Nov

Keywords

Allogeneic response
Co-stimulatory molecule
Cytotoxic T lymphocyte-associated antigen-4
Mixed lymphocyte reaction
Rhesus monkey
T cells

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-012-1026-0

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Kim, J. S., Choi, I., Lee, H. H., Lee, S. J., Na, M., Kim, S. H., Han, J., Bae, J., Choi, S. P., Kim, S. J., Park, C. G., & Chun, T. (2012). Generation and evaluation of the efficacy of rhesus monkey soluble cytotoxic T lymphocyte-associated antigen-4 in the allogeneic mixed lymphocyte reaction. Biotechnology letters, 34(12), 2191-2197. https://doi.org/10.1007/s10529-012-1026-0

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title = "Generation and evaluation of the efficacy of rhesus monkey soluble cytotoxic T lymphocyte-associated antigen-4 in the allogeneic mixed lymphocyte reaction",

abstract = "Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4; CD152) is a transmembrane protein that is structurally similar to CD28. As CTLA-4 has a much higher binding affinity to B7 than CD28, several approaches using soluble CTLA-4 have been tried to down-regulate T cell activity by blocking the interaction between CD28 and B7. We constructed soluble rhesus monkey CTLA-4 immunoglobulin (CTLA-4Ig) containing a critical binding site to B7 combined with a constant Ig heavy chain region in a mammalian system. Flow cytometry analyses indicated that soluble rhesus monkey CTLA-4Ig bound to rhesus monkey CD86 (B7. 2). Moreover, soluble rhesus monkey CTLA-4Ig more effectively blocked the rhesus monkey-rhesus monkey allogeneic mixed lymphocyte reaction compared with that of humans. These results indicate that soluble rhesus monkey CTLA-4Ig may be useful in preclinical trials in a rhesus monkey model.",

keywords = "Allogeneic response, Co-stimulatory molecule, Cytotoxic T lymphocyte-associated antigen-4, Mixed lymphocyte reaction, Rhesus monkey, T cells",

author = "Kim, {Jung Sik} and Inho Choi and Lee, {Han Hyoung} and Lee, {Suk Jun} and Manheum Na and Kim, {Sang Hoon} and Jihye Han and Joonbeom Bae and Choi, {Sang Pil} and Kim, {Sang Joon} and Park, {Chung Gyu} and Taehoon Chun",

note = "Funding Information: We thank the Comit{\'e} de Charente-Maritime de la Ligue Nationale contre le Cancer and the Centre National de Recherche Marocain (programme PROTARS) for financial support. ",

year = "2012",

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doi = "10.1007/s10529-012-1026-0",

language = "English",

volume = "34",

pages = "2191--2197",

journal = "Biotechnology letters",

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T1 - Generation and evaluation of the efficacy of rhesus monkey soluble cytotoxic T lymphocyte-associated antigen-4 in the allogeneic mixed lymphocyte reaction

AU - Kim, Jung Sik

AU - Choi, Inho

AU - Lee, Han Hyoung

AU - Lee, Suk Jun

AU - Na, Manheum

AU - Kim, Sang Hoon

AU - Han, Jihye

AU - Bae, Joonbeom

AU - Choi, Sang Pil

AU - Kim, Sang Joon

AU - Park, Chung Gyu

AU - Chun, Taehoon

N1 - Funding Information: We thank the Comité de Charente-Maritime de la Ligue Nationale contre le Cancer and the Centre National de Recherche Marocain (programme PROTARS) for financial support.

PY - 2012/11

Y1 - 2012/11

N2 - Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4; CD152) is a transmembrane protein that is structurally similar to CD28. As CTLA-4 has a much higher binding affinity to B7 than CD28, several approaches using soluble CTLA-4 have been tried to down-regulate T cell activity by blocking the interaction between CD28 and B7. We constructed soluble rhesus monkey CTLA-4 immunoglobulin (CTLA-4Ig) containing a critical binding site to B7 combined with a constant Ig heavy chain region in a mammalian system. Flow cytometry analyses indicated that soluble rhesus monkey CTLA-4Ig bound to rhesus monkey CD86 (B7. 2). Moreover, soluble rhesus monkey CTLA-4Ig more effectively blocked the rhesus monkey-rhesus monkey allogeneic mixed lymphocyte reaction compared with that of humans. These results indicate that soluble rhesus monkey CTLA-4Ig may be useful in preclinical trials in a rhesus monkey model.

AB - Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4; CD152) is a transmembrane protein that is structurally similar to CD28. As CTLA-4 has a much higher binding affinity to B7 than CD28, several approaches using soluble CTLA-4 have been tried to down-regulate T cell activity by blocking the interaction between CD28 and B7. We constructed soluble rhesus monkey CTLA-4 immunoglobulin (CTLA-4Ig) containing a critical binding site to B7 combined with a constant Ig heavy chain region in a mammalian system. Flow cytometry analyses indicated that soluble rhesus monkey CTLA-4Ig bound to rhesus monkey CD86 (B7. 2). Moreover, soluble rhesus monkey CTLA-4Ig more effectively blocked the rhesus monkey-rhesus monkey allogeneic mixed lymphocyte reaction compared with that of humans. These results indicate that soluble rhesus monkey CTLA-4Ig may be useful in preclinical trials in a rhesus monkey model.

KW - Allogeneic response

KW - Co-stimulatory molecule

KW - Cytotoxic T lymphocyte-associated antigen-4

KW - Mixed lymphocyte reaction

KW - Rhesus monkey

KW - T cells

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SN - 0141-5492

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JF - Biotechnology letters

IS - 12

ER -

Generation and evaluation of the efficacy of rhesus monkey soluble cytotoxic T lymphocyte-associated antigen-4 in the allogeneic mixed lymphocyte reaction

Abstract

Keywords

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