Generation and segregation of hantaviral RNA genomic diploid; implications of reassortant generation mechanism

Sun Whan Park, Dong Hoon Chung, Byung Yoon Ahn, Pyung Woo Lee

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Hantaviruses possess three RNA segments of negative sense. Co-infection of closely related hantaviruses may result in generation of a progeny virus with genomic polyploidy, containing a partial or complete set of genome originated from more than one parental virus. To characterize the formation of viral genomic polyploidy, cultured Vero-E6 cells were co-infected with two closely related hantaviruses, Hantaan and Maaji, and the progeny viruses examined. The genotype of plaque-purified viruses was analyzed by a virus-specific RT-PCR. Seventy percent (67/96) of the progeny virus was categorized as Hantaan and 3.3% (2/96) was classified as Maaji, whereas 20% (21/96) was considered polyploidy as they contained both types of the S RNA segment. Most of the polyploidy progeny viruses were unstable and gave rise to either one of the parental viruses or a reassortant after several rounds of plaque purification. No recombination between the heterologous pair of S RNA was observed for those polyploid viruses during three consecutive plaque-to-plaque passages. These data suggest that the viral polyploidy fomation constitutes a primary mechanism underlying the generation of a newly emerged hantavirus.

Original languageEnglish
Pages (from-to)1017-1025
Number of pages9
JournalJournal of microbiology and biotechnology
Issue number7
Publication statusPublished - 2006 Jul


  • Diploid
  • Genetic reassortment
  • Hantaan virus
  • Hantavirus
  • Maaji virus

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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