Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain

Myungjae Song; Jung Min Lim; Seonwoo Min; Jeong Seok Oh; Dong Young Kim; Jae Sung Woo; Hiroshi Nishimasu; Sung Rae Cho; Sungroh Yoon; Hyongbum Henry Kim

doi:10.1038/s41467-021-25928-2

Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain

Myungjae Song, Jung Min Lim, Seonwoo Min, Jeong Seok Oh, Dong Young Kim, Jae Sung Woo, Hiroshi Nishimasu, Sung Rae Cho, Sungroh Yoon, Hyongbum Henry Kim

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

Although prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency < 1%), hyPE2 enables prime editing with efficiencies ranging from 1.1% to 2.9% at up to 34% of target sequences, potentially facilitating prime editing applications.

Original language	English
Article number	5617
Journal	Nature communications
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41467-021-25928-2
Publication status	Published - 2021 Dec 1

Bibliographical note

Publisher Copyright:
© 2021, The Author(s).

ASJC Scopus subject areas

General Chemistry
General Biochemistry,Genetics and Molecular Biology
General
General Physics and Astronomy

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Cite this

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abstract = "Although prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency < 1%), hyPE2 enables prime editing with efficiencies ranging from 1.1% to 2.9% at up to 34% of target sequences, potentially facilitating prime editing applications.",

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AU - Woo, Jae Sung

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Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain

Abstract

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