Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain

Myungjae Song; Jung Min Lim; Seonwoo Min; Jeong Seok Oh; Dong Young Kim; Jae Sung Woo; Hiroshi Nishimasu; Sung Rae Cho; Sungroh Yoon; Hyongbum Henry Kim

doi:10.1038/s41467-021-25928-2

Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain

Myungjae Song, Jung Min Lim, Seonwoo Min, Jeong Seok Oh, Dong Young Kim, Jae Sung Woo, Hiroshi Nishimasu, Sung Rae Cho, Sungroh Yoon, Hyongbum Henry Kim

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

Although prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency < 1%), hyPE2 enables prime editing with efficiencies ranging from 1.1% to 2.9% at up to 34% of target sequences, potentially facilitating prime editing applications.

Original language	English
Article number	5617
Journal	Nature communications
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41467-021-25928-2
Publication status	Published - 2021 Dec 1

Bibliographical note

Publisher Copyright:
© 2021, The Author(s).

ASJC Scopus subject areas

General Chemistry
General Biochemistry,Genetics and Molecular Biology
General
General Physics and Astronomy

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Cite this

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abstract = "Although prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency < 1%), hyPE2 enables prime editing with efficiencies ranging from 1.1% to 2.9% at up to 34% of target sequences, potentially facilitating prime editing applications.",

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AU - Min, Seonwoo

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AU - Woo, Jae Sung

AU - Nishimasu, Hiroshi

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AU - Kim, Hyongbum Henry

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Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain

Abstract

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