Abstract
Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.
Original language | English |
---|---|
Pages (from-to) | 72-75 |
Number of pages | 4 |
Journal | Stem Cell Research |
Volume | 26 |
DOIs | |
Publication status | Published - 2018 Jan |
Bibliographical note
Funding Information:This research was supported by the Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP), Republic of Korea (No. NRF-2015R1C1A2A01051471).
Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP), Republic of Korea (No. NRF-2015R1C1A2A01051471 ).
Publisher Copyright:
© 2017 The Authors
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology