Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system

Hyemin Kim, Ji Woo Kim, So Jin Kim, Young Jun Choi, Dae Sung Kim, Han Jin Park

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.

    Original languageEnglish
    Pages (from-to)72-75
    Number of pages4
    JournalStem Cell Research
    Volume26
    DOIs
    Publication statusPublished - 2018 Jan

    Bibliographical note

    Funding Information:
    This research was supported by the Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP), Republic of Korea (No. NRF-2015R1C1A2A01051471).

    Funding Information:
    This research was supported by the Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP), Republic of Korea (No. NRF-2015R1C1A2A01051471 ).

    Publisher Copyright:
    © 2017 The Authors

    ASJC Scopus subject areas

    • Developmental Biology
    • Cell Biology

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