Genetic analysis of Borrelia burgdorferi sensu lato in Korea using genomic hybridization and 16S rRNA gene sequence determination

Sun Ho Kee, Jung Hoon Yoon, Hee Bok Oh, Yong Ha Park, Yoon Won Kim, Min Kee Cho, Kyung Seok Park, Woo Hyun Chang

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Nine Borrelia burgdorferi sensu lato isolated in Korea were subjected to genomic hybridization using 16S rRNA gene probe and specific restriction patterns (HindIII and EcoRV) led these nine Borrelia into five subtypes. The evolutionary relationships of the five isolates corresponding to five RFLP groups were measured through the sequence determination of 16S rRNA gene and phylogenetic analysis. The isolates 935T (group I), 934U and 17Y (Group IIa, IIb) were well clustered with B. garinii and B. afzelii. 5MT and 9MT strains (Group IIIa and Group IIIb) formed a common branch shared with B. afzelii cluster although the evolutionary distance was rather long. So, most of B. burgdorferi sensu lato in Korea was B. afzelii or B. afzelii-related group and some minor group such as B. garinii also existed.

Original languageEnglish
Pages (from-to)599-605
Number of pages7
JournalMicrobiology and Immunology
Volume40
Issue number9
DOIs
Publication statusPublished - 1996
Externally publishedYes

Keywords

  • 16S rRNA
  • Borrelia burgdorferi sensu lato
  • RFLP

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Fingerprint

Dive into the research topics of 'Genetic analysis of Borrelia burgdorferi sensu lato in Korea using genomic hybridization and 16S rRNA gene sequence determination'. Together they form a unique fingerprint.

Cite this