Genetic analysis of Borrelia burgdorferi sensu lato in Korea using genomic hybridization and 16S rRNA gene sequence determination

Sun Ho Kee; Jung Hoon Yoon; Hee Bok Oh; Yong Ha Park; Yoon Won Kim; Min Kee Cho; Kyung Seok Park; Woo Hyun Chang

doi:10.1111/j.1348-0421.1996.tb01115.x

Genetic analysis of Borrelia burgdorferi sensu lato in Korea using genomic hybridization and 16S rRNA gene sequence determination

Sun Ho Kee
, Jung Hoon Yoon
, Hee Bok Oh
, Yong Ha Park
, Yoon Won Kim
, Min Kee Cho
, Kyung Seok Park
, Woo Hyun Chang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Nine Borrelia burgdorferi sensu lato isolated in Korea were subjected to genomic hybridization using 16S rRNA gene probe and specific restriction patterns (HindIII and EcoRV) led these nine Borrelia into five subtypes. The evolutionary relationships of the five isolates corresponding to five RFLP groups were measured through the sequence determination of 16S rRNA gene and phylogenetic analysis. The isolates 935T (group I), 934U and 17Y (Group IIa, IIb) were well clustered with B. garinii and B. afzelii. 5MT and 9MT strains (Group IIIa and Group IIIb) formed a common branch shared with B. afzelii cluster although the evolutionary distance was rather long. So, most of B. burgdorferi sensu lato in Korea was B. afzelii or B. afzelii-related group and some minor group such as B. garinii also existed.

Original language	English
Pages (from-to)	599-605
Number of pages	7
Journal	Microbiology and Immunology
Volume	40
Issue number	9
DOIs	https://doi.org/10.1111/j.1348-0421.1996.tb01115.x
Publication status	Published - 1996

Keywords

16S rRNA
Borrelia burgdorferi sensu lato
RFLP

ASJC Scopus subject areas

Microbiology
Immunology
Virology

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10.1111/j.1348-0421.1996.tb01115.x

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title = "Genetic analysis of Borrelia burgdorferi sensu lato in Korea using genomic hybridization and 16S rRNA gene sequence determination",

abstract = "Nine Borrelia burgdorferi sensu lato isolated in Korea were subjected to genomic hybridization using 16S rRNA gene probe and specific restriction patterns (HindIII and EcoRV) led these nine Borrelia into five subtypes. The evolutionary relationships of the five isolates corresponding to five RFLP groups were measured through the sequence determination of 16S rRNA gene and phylogenetic analysis. The isolates 935T (group I), 934U and 17Y (Group IIa, IIb) were well clustered with B. garinii and B. afzelii. 5MT and 9MT strains (Group IIIa and Group IIIb) formed a common branch shared with B. afzelii cluster although the evolutionary distance was rather long. So, most of B. burgdorferi sensu lato in Korea was B. afzelii or B. afzelii-related group and some minor group such as B. garinii also existed.",

keywords = "16S rRNA, Borrelia burgdorferi sensu lato, RFLP",

author = "Kee, \{Sun Ho\} and Yoon, \{Jung Hoon\} and Oh, \{Hee Bok\} and Park, \{Yong Ha\} and Kim, \{Yoon Won\} and Cho, \{Min Kee\} and Park, \{Kyung Seok\} and Chang, \{Woo Hyun\}",

year = "1996",

doi = "10.1111/j.1348-0421.1996.tb01115.x",

language = "English",

volume = "40",

pages = "599--605",

journal = "Microbiology and Immunology",

issn = "0385-5600",

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TY - JOUR

T1 - Genetic analysis of Borrelia burgdorferi sensu lato in Korea using genomic hybridization and 16S rRNA gene sequence determination

AU - Kee, Sun Ho

AU - Yoon, Jung Hoon

AU - Oh, Hee Bok

AU - Park, Yong Ha

AU - Kim, Yoon Won

AU - Cho, Min Kee

AU - Park, Kyung Seok

AU - Chang, Woo Hyun

PY - 1996

Y1 - 1996

N2 - Nine Borrelia burgdorferi sensu lato isolated in Korea were subjected to genomic hybridization using 16S rRNA gene probe and specific restriction patterns (HindIII and EcoRV) led these nine Borrelia into five subtypes. The evolutionary relationships of the five isolates corresponding to five RFLP groups were measured through the sequence determination of 16S rRNA gene and phylogenetic analysis. The isolates 935T (group I), 934U and 17Y (Group IIa, IIb) were well clustered with B. garinii and B. afzelii. 5MT and 9MT strains (Group IIIa and Group IIIb) formed a common branch shared with B. afzelii cluster although the evolutionary distance was rather long. So, most of B. burgdorferi sensu lato in Korea was B. afzelii or B. afzelii-related group and some minor group such as B. garinii also existed.

AB - Nine Borrelia burgdorferi sensu lato isolated in Korea were subjected to genomic hybridization using 16S rRNA gene probe and specific restriction patterns (HindIII and EcoRV) led these nine Borrelia into five subtypes. The evolutionary relationships of the five isolates corresponding to five RFLP groups were measured through the sequence determination of 16S rRNA gene and phylogenetic analysis. The isolates 935T (group I), 934U and 17Y (Group IIa, IIb) were well clustered with B. garinii and B. afzelii. 5MT and 9MT strains (Group IIIa and Group IIIb) formed a common branch shared with B. afzelii cluster although the evolutionary distance was rather long. So, most of B. burgdorferi sensu lato in Korea was B. afzelii or B. afzelii-related group and some minor group such as B. garinii also existed.

KW - 16S rRNA

KW - Borrelia burgdorferi sensu lato

KW - RFLP

UR - https://www.scopus.com/pages/publications/0029835330

U2 - 10.1111/j.1348-0421.1996.tb01115.x

DO - 10.1111/j.1348-0421.1996.tb01115.x

M3 - Article

C2 - 8908602

AN - SCOPUS:0029835330

SN - 0385-5600

VL - 40

SP - 599

EP - 605

JO - Microbiology and Immunology

JF - Microbiology and Immunology

IS - 9

ER -

Genetic analysis of Borrelia burgdorferi sensu lato in Korea using genomic hybridization and 16S rRNA gene sequence determination

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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